Identification of transferrin receptor in cervical and endometrial tissues

Mizuuchi, Hidemitsu; Kudo, Ryuichi; Tamura, H.; Tsukahara, Kunihiko; Tsumura, Noritoshi; Kumai, Kentoku; Sato, Kuniko

doi:10.1016/0020-7292(89)90626-7

Cited by 1 publication

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2A). The increased level of transferrin receptor in vitro correlates with in vivo data showing increased transferrin receptors in high-grade cervical lesions and cancers as measured by immunohistochemistry (14)(15)(16). Thus, the increase in transferrin receptors is not an artifact of in vitro culturing of the cells.…”

Section: Resultssupporting

confidence: 74%

“…In a subsequent study, the cervical cancer cell line HeLa was also shown to be sensitive to DHA although a mechanism for cell death was not elucidated (8). We speculated that both cervical cancer and immortalized cell lines, which are sensitized to apoptotic cell death by the expression of the E6 and E7 oncogenes (9-13) and overexpress the transferrin receptor (14)(15)(16), might be killed by these antimalarial compounds.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dihydroartemisinin Is Cytotoxic to Papillomavirus-Expressing Epithelial Cells In vitro and In vivo

et al. 2005

View full text Add to dashboard Cite

Nearly all cervical cancers are etiologically attributable to human papillomavirus (HPV) infection and pharmaceutical treatments targeting HPV-infected cells would be of great medical benefit. Because many neoplastic cells (including cervical cancer cells) overexpress the transferrin receptor to increase their iron uptake, we hypothesized that irondependent, antimalarial drugs such as artemisinin might prove useful in treating HPV-infected or transformed cells. We tested three different artemisinin compounds and found that dihydroartemisinin (DHA) and artesunate displayed strong cytotoxic effects on HPV-immortalized and transformed cervical cells in vitro with little effect on normal cervical epithelial cells. DHA-induced cell death involved activation of the mitochondrial caspase pathway with resultant apoptosis. Apoptosis was p53 independent and was not the consequence of drug-induced reductions in viral oncogene expression. Due to its selective cytotoxicity, hydrophobicity, and known ability to penetrate epithelial surfaces, we postulated that DHA might be useful for the topical treatment of mucosal papillomavirus lesions. To test this hypothesis, we applied DHA to the oral mucosa of dogs that had been challenged with the canine oral papillomavirus. Although applied only intermittently, DHA strongly inhibited viral-induced tumor formation. Interestingly, the DHA-treated, tumor-negative dogs developed antibodies against the viral L1 capsid protein, suggesting that DHA had inhibited tumor growth but not early rounds of papillomavirus replication. These findings indicate that DHA and other artemisinin derivatives may be useful for the topical treatment of epithelial papillomavirus lesions, including those that have progressed to the neoplastic state. (Cancer Res 2005; 65(23): 10854-61)

show abstract

Section: Resultssupporting

confidence: 74%