Identification of toxicity effects of Cu₂O materials onC. elegansas a function of environmental ionic composition

Abstract

C. elegans worms encountering Cu2O particles can either avoid and survive or they can ingest them and experience toxic effects. Phosphate induces particle oxidation and Cu ion precipitation, improving survival even after ingestion.

Facile synthesis of Cu N-lauroyl sarcosinate nanozymes with laccase-mimicking activity and identification of toxicity effects for C. elegans

“…Since sewage contains different metal ions, 15 we investigated whether the combination of other metal ions with Ls exhibited a laccase-like activity. Although precipitation with Ls ligand was observed with all tested metal ions ( Fig.…”

Dysfunction in atox-1 and ceruloplasmin alters labile Cu levels and consequently Cu homeostasis in C. elegans

“…During lethality testing we noticed that worms exposed to 2 mM Cu started to display shortened and thinner phenotype, which indicated the onset of a developmental delay. Concentrations above 2 mM were not considered, since worms were previously shown to avoid higher amounts of Cu, as described in Guo et al (2015) and Munro et al (2020).…”

Directed movement changes coexistence outcomes in heterogeneous environments

“…We also ran simulations, as described below, with different initial numbers (6 or 12 initial worms) and initial locations (all placed at the centre or equally distributed across the plate), confirming that the initial number of animals or their initial position on the plate had no effect on the predictions (Figures S2 and S3 in Appendix S1). Every 4 days, four fields were randomly selected on each plate using a Leica M165FC fluorescence microscope with a FLIR Grasshopper 3 USB3 camera, as described in Collins et al (2016) and Munro et al (2020). The numbers of fluorescent and non-fluorescent strains in each field were counted by using a custom script in MATLAB (2018).…”