Identification of Tobacco Proteins Associated with the Stem-Loop 1 RNAs of Potato virus X

Two homologous host proteins interact with potato virus X RNAs and CPs and affect viral replication and movement

“…As noted, we previously detected 24 N. benthamiana proteins that interact with SL1 RNAs of PVX 21 . Of the 24 proteins, we selected two that are homologous to known potyviral CP interacting protein 2a (CPIP2a) from N. tabacum 23 .…”

“…Several host proteins have been identified that interact with PVX viral proteins including NbPCIP1, TIP, NbMPB2Cb, NbDnaJ, eEF1A, and eEF1Bβ 16 17 18 19 20 . Moreover, researchers have used Northwestern blot followed by MALDI TOP MS to identify tobacco proteins that bind to PVX 5′ SL RNAs 21 22 .…”

“…In this study, we characterized two homologous host proteins that were previously determined to interact with SL RNAs of PVX 21 . Electrophoretic mobility shift assay (EMSA) as well as BiFC were used to confirm the interactions of host proteins and PVX viral components.…”

Multiple functions of capsid proteins in (+) stranded RNA viruses during plant–virus interactions

“…The 5 cis-acting stem-loop 1 (SL1) element on PVX RNA is involved in replication, encapsidation, translation, and cell-to-cell movement. The PVX CP binds to both plus and minus strands of SL1 RNA domain alongside with 24 N. benthamiana host proteins (Cho et al, 2012). Two proteins, NbMPB2Cb and NbMBF1, can bind to both SL1(+) and (−) RNAs whereas NbCPIP2a interacts only with SL1(+) RNA.…”

Identification of the Capsid Protein-binding Region of the SL1(+) RNA Located at the 5' Region of the Potato virus X Genome

“…Recent research indicates that SL1 RNA may have an additional role(s) in the PVX infection cycle by specifically interacting with host proteins (Cho et al, 2012b(Cho et al, , 2012c. Several host proteins play roles in PVX replication and movement by interacting with SL1 RNAs and PVX CP (Cho et al, 2012a). These data indicate that multiple features of SL1 are critical for PVX infection cycles.…”