Identification of tiagabine degradation products using liquid chromatography with electrospray ionization multistage mass spectrometry and ultra‐performance liquid chromatography/high‐resolution mass spectrometry

Hubert‐Roux, Marie; Sarakha, Mohamed; Sughir, Abdussalam; Lahiani-Skiba, Malika; Olivier-Chanu, Fabienne; Levacher, Vincent; Lange, Catherine

doi:10.1002/rcm.5329

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…Rustum et al 11 proposed a chiral HPLC method to quantify the S-(+)-enantiomer of TBN in its bulk drug and to separate its S (+), R (−)-enantiomers as well as two chiral precursors. Hubert-Roux et al 12 identified degradation products of TBN using LC-MS-MS and UPLC-HRMS. Rajasingam et al 13 studied the stress degradation and proposed an RP-HPLC method to determine TBN in presence of its degradation products.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Quantification of Tiagabine and Its Related Substance by a Stabilityindicating Rp-HPLC Method

Kishore¹,

Ramana²,

Nadh³

et al. 2021

RJC

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The proposed study is the development of the RP-HPLC method for simultaneous quantification of tiagabine (TBN) and its related substance -A (TBN RS-A). The method was validated for its applicability both in bulk drug and tablet dosage form. For an isocratic elution, a mobile phase comprising of methanol: 0.1 mM acetate buffer mixture (65:45 v/v, pH 5.6) was used at 1 ml/min flow rate and ProntoSIL ODS C18 column (250mm × 4.5 mm; 5µm) column. At 240 nm as max, sharp peaks of TBN RS-A and TBN were observed at 3.7 and 5.2 min respectively. As per the ICH guidelines, the method was validated. Validation of the method was done as per the guidelines of ICH. Linear regression for the calibration curve was carried out for concentration ranges of 75-450 and 1-6 µg/mL respectively for TBN and TBN RS-A. The respective detection limits (LOQ and LOD) of the TBN and RS-A were found to be (2.014 and 0.032 µg/ml) and (6.103 and 0.098 µg/ml). The method was successfully extended for stability-indicating studies under different stress conditions.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Quantification of Tiagabine and Its Related Substance by a Stabilityindicating Rp-HPLC Method

Kishore¹,

Ramana²,

Nadh³

et al. 2021

RJC

View full text Add to dashboard Cite

show abstract

Interpretation of tandem mass spectra of antiepileptic drugs using accurate-m/z data and m/z-shifts with stable-isotope labeled analogues

Niessen¹

2020

International Journal of Mass Spectrometry

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Degradation pathways study of the natriuretic and β-adrenoceptor antagonist tienoxolol using liquid chromatography–electrospray ionization multistage mass spectrometry

Gana

Dugay

Henriet

et al. 2014

Journal of Pharmaceutical and Biomedical Analysis

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Identification of tiagabine degradation products using liquid chromatography with electrospray ionization multistage mass spectrometry and ultra‐performance liquid chromatography/high‐resolution mass spectrometry

Abstract: Nine degradation products of TGB were identified. Some diagnostic MS/MS product ions, characteristic of the piperidine or thiophene moiety, were highlighted.

Cited by 4 publications

References 15 publications

Simultaneous Quantification of Tiagabine and Its Related Substance by a Stabilityindicating Rp-HPLC Method

Simultaneous Quantification of Tiagabine and Its Related Substance by a Stabilityindicating Rp-HPLC Method

Interpretation of tandem mass spectra of antiepileptic drugs using accurate-m/z data and m/z-shifts with stable-isotope labeled analogues

Degradation pathways study of the natriuretic and β-adrenoceptor antagonist tienoxolol using liquid chromatography–electrospray ionization multistage mass spectrometry

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