Identification of three novel antioxidative peptides from Auxenochlorella pyrenoidosa protein hydrolysates based on a peptidomics strategy

Zhu, Zihao; Chen, Yuyang; Jia, Nan; Zhang, Wenhan; Hou, Hu; Xue, Changhu; Wang, Yanchao

doi:10.1016/j.foodchem.2021.131849

Cited by 27 publications

(9 citation statements)

References 40 publications

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“…As a hydrogen donor, the peptide pairs with the single electron of DPPH that exists in the form of free radicals in solution to complete the free radical scavenging [ 47 ]. The six identified peptides are rich in amino acid residues such as Pro, Tyr and Ala, which are reported to be effective hydrogen donors and play an important role in the antioxidant capacity of peptides [ 48 , 49 ]. Based on the simulation results, the identified peptides and DPPH molecules had strong hydrophobic interactions and hydrogen bonds, so the peptides had strong DPPH free radical scavenging activity.…”

Section: Resultsmentioning

confidence: 99%

Identification of Antioxidant Peptides Derived from Tilapia (Oreochromis niloticus) Skin and Their Mechanism of Action by Molecular Docking

Zhang

Liu

et al. 2022

Foods

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Antioxidants, which can activate the body’s antioxidant defence system and reduce oxidative stress damage, are important for maintaining free radical homeostasis between oxidative damage and antioxidant defence. Six antioxidant peptides (P1–P6) were isolated and identified from the enzymatic hydrolysate of tilapia skin by ultrafiltration, reversed-phase high-performance liquid chromatography (RP-HPLC) and liquid chromatography–tandem mass spectrometry (LC–MS/MS). Moreover, the scavenging mechanism of the identified peptides against DPPH (2,2-Diphenyl-1-picrylhydrazyl) and ABTS (2-azido-bis (3-ethylbenzothiazoline-6-sulfonic acid) was studied by molecular docking. It was found that Pro, Ala and Tyr were the characteristic amino acids for scavenging free radicals, and hydrogen bonding and hydrophobic interactions were the main interactions between the free radicals and antioxidant peptides. Among them, the peptide KAPDPGPGPM exhibited the highest DPPH free radical scavenging activity (IC50 = 2.56 ± 0.15 mg/mL), in which the hydrogen bond between the free radical DDPH and Thr-6 was identified as the main interaction, and the hydrophobic interactions between the free radical DDPH and Ala, Gly and Pro were also identified. The peptide GGYDEY presented the highest scavenging activity against ABTS (IC50 = 9.14 ± 0.08 mg/mL). The key structures for the interaction of this peptide with the free radical ABTS were identified as Gly-1 and Glu-5 (hydrogen bond sites), and the amino acids Tyr and Asp provided hydrophobic interactions. Furthermore, it was determined that the screened peptides are suitable for applications as antioxidants in the food industry, exhibit good water solubility and stability, are likely nonallergenic and are nontoxic. In summary, the results of this study provide a theoretical structural basis for examining the mechanism of action of antioxidant peptides and the application of enzymatic hydrolysates from tilapia skin.

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Section: Resultsmentioning

confidence: 99%

Identification of Antioxidant Peptides Derived from Tilapia (Oreochromis niloticus) Skin and Their Mechanism of Action by Molecular Docking

Zhang

Liu

et al. 2022

Foods

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“…The hydrolysate was prepared using a previously described method by Zhu et al with slight modifications . The 5 g of dry GBSC protein isolate was mixed with 500 mL of water (w/v), and the pH was adjusted to 7.50 using 1 M NaOH.…”

Section: Methodsmentioning

confidence: 99%

Screening and Evaluation of Novel α-Glucosidase Inhibitory Peptides from Ginkgo biloba Seed Cake Based on Molecular Docking Combined with Molecular Dynamics Simulation

Deng

Zhang

et al. 2023

J. Agric. Food Chem.

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Food-derived α-glucosidase inhibitory peptides have gained significant interest in treating type 2 diabetes mellitus (T2DM) owing to their favorable safety profiles. Molecular docking combined with molecular dynamics simulation was performed to screen α-glucosidase inhibitory peptides from Ginkgo biloba seed cake (GBSC), and two novel peptides (Met-Pro-Gly-Pro-Pro (MPGPP) and Phe-Ala-Pro-Ser-Trp (FAPSW)) were acquired. The results of molecular docking and molecular dynamics simulation suggested that FAPSW and MPGPP could generate stable complexes with 3wy1, and the electrostatic and van der Waals forces played contributory roles in FAPSW and MPGPP binding to 3wy1. The α-glucosidase inhibition assay corroborated that FAPSW and MPGPP had good α-glucosidase inhibition capacity, with IC50 values of 445.34 ± 49.48 and 1025.68 ± 140.78 μM, respectively. In vitro simulated digestion results demonstrated that FAPSW and MPGPP strongly resisted digestion. These findings lay a theoretical foundation for FAPSW and MPGPP in treating T2DM.

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“…The sequence analysis of the sample was performed with reference to the previous method . In brief, the fraction P3 was dissolved in 0.1% aqueous trifluoroacetic acid and loaded onto an activated (50% acetonitrile) and equilibrated (0.1% aqueous trifluoroacetic acid) ZipTip C18 column, desalted with H 2 O (0.1% trifluoroacetic acid), eluted with 50% acetonitrile (0.1% trifluoroacetic acid), and then freeze-dried.…”

Section: Methodsmentioning

confidence: 99%

“…The sequence analysis of the sample was performed with reference to the previous method. 13 In brief, the fraction P3 was dissolved in 0.1% aqueous trifluoroacetic acid and loaded onto an activated (50% acetonitrile) and equilibrated (0.1% aqueous trifluoroacetic acid) ZipTip C18 column, desalted with H 2 O (0.1% trifluoroacetic acid), eluted with 50% acetonitrile (0.1% trifluoroacetic acid), and then freeze-dried. LC−MS/MS analysis was performed by using an Ultimate 3000 UPLC system (Thermo, Waltham, MA, USA) equipped with an ACQUITY UPLC Peptide CSH C18 column (1 mm × 150 mm, 1.7 μm) coupled to a Q Exactive mass spectrometer.…”

Section: Purification Of Scihmentioning

confidence: 99%

Novel Peptides Derived from Sea Cucumber Intestine Promotes Osteogenesis by Upregulating Integrin-Mediated Transdifferentiation of Growth Plate Chondrocytes to Osteoblasts

Hao

Tian

Feng

et al. 2022

J. Agric. Food Chem.

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The sea cucumber intestine is a major by-product of sea cucumber processing and contains high levels of protein. In this study, we isolated and identified 28 novel osteogenic peptides from sea cucumber intestinal hydrolysis by the activity-tracking method for the first time. In vitro experimental results showed that compared with high molecular weight, the peptides from sea cucumber intestine (SCIP) with molecular weight <3 kDa more significantly promoted the proliferation and mineralized nodules of MC3T3-E1 cell and exhibited potential integrin binding capacity. In vivo experimental results showed that the SCIP supplement significantly increased the longitudinal bone length and elevated the height of the growth plate (especially the hypertrophic zone, 37.2%, p < 0.01) in adolescent mice. Further, immunofluorescence labeling results indicated that the SCIP supplement increased chondrocyte transdifferentiate to osteoblast in the growth plate close to the diaphysis. Mechanistically, transcriptome analysis revealed that the SCIP supplement induced the dedifferentiation of chondrocyte to osteoprogenitor cell via integrin-mediated histone acetylation and then redifferentiated to osteoblast via integrin-mediated Wnt/β-catenin signaling. These results reported for the first time that sea cucumber intestine had the potential to develop into a dietary supplement for promoting osteogenic, and provide new evidence for the mechanism of dietary promotes chondrocyte to osteoblast transdifferentiation.

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Identification of three novel antioxidative peptides from Auxenochlorella pyrenoidosa protein hydrolysates based on a peptidomics strategy

Cited by 27 publications

References 40 publications

Identification of Antioxidant Peptides Derived from Tilapia (Oreochromis niloticus) Skin and Their Mechanism of Action by Molecular Docking

Identification of Antioxidant Peptides Derived from Tilapia (Oreochromis niloticus) Skin and Their Mechanism of Action by Molecular Docking

Screening and Evaluation of Novel α-Glucosidase Inhibitory Peptides from Ginkgo biloba Seed Cake Based on Molecular Docking Combined with Molecular Dynamics Simulation

Novel Peptides Derived from Sea Cucumber Intestine Promotes Osteogenesis by Upregulating Integrin-Mediated Transdifferentiation of Growth Plate Chondrocytes to Osteoblasts

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