Identification of three Macrobrachium species from the Kii Peninsula, Japan using PCR-RFLP

Abstract

We developed a method for discriminating among three Macrobrachium species (M. nipponense, M. formosense and M. japonicum) that inhabit rivers of the Kii Peninsula, Japan. Using the restriction enzyme, Bsp1286I, we performed polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis on the cytochrome oxidase subunit I (CO I) region of the mitochondrial DNA. Our analysis clearly discriminated among the three Macrobrachium species (62 individuals), and was consistent with classifi cation based on morphological characters. There was no intraspecifi c variation in the specifi c restriction profi les. In addition, we were able to classify 95 morphologically unidentifi ed individuals, including juvenile or female individuals, based on their specifi c restriction profi les. Our results suggest that PCR-RFLP may be used to discriminate among these three Macrobrachium species. The method is particularly useful for classifying juvenile or female individuals that cannot be identifi ed morphologically.

Occurrence of two exotic decapods,Macrobrachium nipponense(de Haan, 1849) andProcambarus virginalisLyko, 2017, in Ukrainian waters

“…To perform our phylogenetic analysis, previously published GenBank nucleotide sequences (Salman et al, 2006;Feng et al, 2008;Wowor et al, 2009;Martin et al, 2010aMartin et al, , 2010bFilipová et al, 2011;Liu and Cui, 2011;Aoki et al, 2013;Bohman et al, 2013;Quan et al, 2014;Vojkovská et al, 2014;Vogt et al, 2015;Usio et al, 2017Cui et al, 2018Zheng et al, 2019) were combined with our molecular data. Localities and GenBank accession numbers of sequences used in the present study are listed in Supplementary Table S1.…”

A simple method for species identification of the ghost crabs using PCR-RFLP

“…Many studies have demonstrated the usefulness of species identification based on molecular techniques (Ivanova et al 2007, Smith et al 2008, Che et al 2012). Among the molecular techniques for species identification, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is frequently used (Tosuji & Sato 2012, Aoki et al 2013, Yamamoto et al 2018, Nakai et al 2023 since it is easily done without a DNA sequencer if PCR and electrophoresis devices are available. In this study, therefore, we developed a PCR-RFLP method for identifying the ghost crab species that appear in the temperate regions of Japan Islands.…”