Identification of three linear B cell epitopes against non-structural protein 3ABC of FMDV using monoclonal antibodies

Shao, Jing; Chen, Danian; Chang, Yung-Fu; Chang, Huiyun; Zhang, Yongguang

doi:10.1007/s00253-019-10081-0

Cited by 6 publications

(23 citation statements)

References 34 publications

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“…Over the last few decades, the three-dimensional structures of various FMDV serotypes and subtypes have been solved by X-ray crystallography, enabling a deeper understanding of FMDV antigenicity [ 62 ]. Despite their sequence variability, all FMDVs share an icosahedral capsid made up of 60 protomers, each consisting of four structural, VP1–VP4 (VP1–3 external, VP4 internal), plus eleven mature non-structural proteins (NSPs) L a , L ab , 2A, 2B, 2C, 3A, 3B 1–3 , 3C and 3D, involved in viral replication and interaction with host cells [ 63 , 64 ]. External proteins VP1–3 consist of structurally similar cores containing eight-stranded β-sandwiches of two four-stranded β-sheets connected by surface-exposed loops.…”

Section: Fmdv Antigenic Structurementioning

confidence: 99%

Peptide-Based Vaccines: Foot-and-Mouth Disease Virus, a Paradigm in Animal Health

et al. 2021

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Vaccines are considered one of the greatest global health achievements, improving the welfare of society by saving lives and substantially reducing the burden of infectious diseases. However, few vaccines are fully effective, for reasons ranging from intrinsic limitations to more contingent shortcomings related, e.g., to cold chain transport, handling and storage. In this context, subunit vaccines where the essential antigenic traits (but not the entire pathogen) are presented in rationally designed fashion have emerged as an attractive alternative to conventional ones. In particular, this includes the option of fully synthetic peptide vaccines able to mimic well-defined B- and T-cell epitopes from the infectious agent and to induce protection against it. Although, in general, linear peptides have been associated to low immunogenicity and partial protection, there are several strategies to address such issues. In this review, we report the progress towards the development of peptide-based vaccines against foot-and-mouth disease (FMD) a highly transmissible, economically devastating animal disease. Starting from preliminary experiments using single linear B-cell epitopes, recent research has led to more complex and successful second-generation vaccines featuring peptide dendrimers containing multiple copies of B- and T-cell epitopes against FMD virus or classical swine fever virus (CSFV). The usefulness of this strategy to prevent other animal and human diseases is discussed.

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Section: Fmdv Antigenic Structurementioning

confidence: 99%

Peptide-Based Vaccines: Foot-and-Mouth Disease Virus, a Paradigm in Animal Health

et al. 2021

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“…The mAbs against the 3ABC protein, designated 2G5 and 9E2, were obtained at our laboratory, and their minimally identified epitopes are " 92 EYIEKA 97 ", which is located in 3A, and " 23 EGPYAGPLE 31 ", which is located in 3B (W. Liu et al, 2019). MAbs 2G5 and 9E2 were largely produced by injecting hybridomas into the peritoneal cavities of BALB/c mice and purified by affinity protein G column chromatography.…”

Section: Antigen and Antibodiesmentioning

confidence: 99%

“…Foot-and-mouth disease (FMD) is a highly infectious and economically devastating viral disease of clovenhoofed animals. The causative agent, FMD virus (FMDV), has a positive-sense and single-stranded RNA that codes for four structural proteins (SPs: VP4, VP2, VP3 and VP1) and 10 non-structural proteins (NSPs: L, 2A, 2B, 2C, 3A, 3B, 3C, 3D, 3AB and 3ABC) (Jaworski et al, 2011;W. Liu et al, 2019;Z.…”

Section: Introductionmentioning

confidence: 99%

“…However, vaccination with inactivated vaccines raises other issues, such as the differentiation of FMDV-infected from vaccinated animals (DIVA) and the production of carrier animals to shed the virus (Sharma et al, 2012;Uttenthal et al, 2010). Therefore, the detection of antibodies against NSPs is the preferred diagnostic method for DIVA and helps to identify subclinical infections, evaluate the prevalence of infection and control the disease (Lu et al, 2010) because most NSPs are removed from the inactivated vaccine by a series of purification methods during production (Fu et al, 2014;W. Liu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…K. Mohapatra, Pandey, Sanyal, & Pattnaik, 2011), 2C3AB (Lu et al, 2010;Wu et al, 2011), or 3ABC (Brocchi et al, 2006;Bruderer et al, 2004;De Diego, Brocchi, Mackay, & De Simone, 1997;Hosamani et al, 2015;Lu et al, 2007;Robiolo et al, 2006). In particular, 3ABC is the most appropriate indicator because of its high immunogenicity and its relatively low abundance in infected cell lysates (W. Liu et al, 2019;Z. Liu et al, 2017;Lu et al, 2010;Robiolo et al, 2006;Van Dreumel et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Development of a competitive chemiluminescence immunoassay using a monoclonal antibody recognizing 3B of foot-and-mouth disease virus for differentiating infected from vaccinated animals

Zhang¹,

Yang²,

Li³

et al. 2021

Preprint

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Foot-and-mouth disease (FMD) is a devastating animal disease. Differentiation of infected from vaccinated animals (DIVA) is very important for confirming suspected cases, evaluating the prevalence of infection, certifying animals for trade and controlling the disease. In this study, a competitive chemiluminescence immunoassay (3B-cCLIA) for DIVA was developed for the rapid detection of antibodies against non-structural proteins (NSPs) in different species of livestock animals using monoclonal antibody 9E2 as a competitive antibody that recognizes NSP 3B, which only needs to be washed once and takes 15 min. The cut-off (50%), diagnosis sensitivity (97.20%, 95.71%, and 96.15%) and diagnosis specificity (99.51%, 99.43%, and 98.36) of the assay were estimated by testing a panel of known background sera from swine, cattle and sheep. The accuracy rate of 3B-cCLIA was also validated and compared with that of two commercial diagnostic kits. The early diagnostic performance showed that antibodies to NSPs occurred later (approximately 1–2 days) than antibodies to structural proteins. Furthermore, NSP antibodies present in animals vaccinated multiple times (false-positive), especially in cattle and sheep, were confirmed, and the false-positive rate increased with the number of vaccinations. These results indicated that 3B-cCLIA is suitable to rapidly detect antibodies against FMDV NSP 3B in a wide range of species for DIVA.

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Development of an Indirect Chemiluminescence Immunoassay Using a Multiepitope Recombinant Protein To Specifically Detect Antibodies against Foot-and-Mouth Disease Virus Serotype O in Swine

et al. 2021

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Foot-and-mouth disease virus (FMDV) has led to serious losses in animal husbandry worldwide. Seromonitoring of FMDV post-vaccination is important for the control and eradication of foot-and-mouth disease (FMD) in vaccinated regions and countries. However, many commercial kits present high false-positive rates. In this study, a multi-epitope-based indirect chemiluminescence immunoassay (ME-CLIA) was developed for specifically detecting antibodies against FMDV serotype O in swine sera. The developed method presented high diagnostic sensitivity, excellent diagnostic specificity, and could detect a broad spectrum of antibodies against FMDV serotype O. The diagnostic performance, accuracy rate, and analytical sensitivity of ME-CLIA were compared with those of three commercial kits. The immune protection value of multiple-epitope recombinant vaccine detected using ME-CLIA was preliminarily determined by observation of clinical symptoms post-immunization challenge, the results of which indicated that the ME-CLIA can be employed as a matching detection method for evaluating multiple-epitope recombinant vaccine. The percent positive values of ME-CLIA determined using swine vaccinated with inactivated vaccine were significantly positively correlated with the titers of liquid-phase blocking ELISA (r = 0.8361, p < 0.0001). These results indicated that ME-CLIA is suitable for detection of antibodies against FMDV serotype O in swine and for potency evaluation of multiple-epitope and inactivated vaccines.

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Identification of three linear B cell epitopes against non-structural protein 3ABC of FMDV using monoclonal antibodies

Cited by 6 publications

References 34 publications

Peptide-Based Vaccines: Foot-and-Mouth Disease Virus, a Paradigm in Animal Health

Peptide-Based Vaccines: Foot-and-Mouth Disease Virus, a Paradigm in Animal Health

Development of a competitive chemiluminescence immunoassay using a monoclonal antibody recognizing 3B of foot-and-mouth disease virus for differentiating infected from vaccinated animals

Development of an Indirect Chemiluminescence Immunoassay Using a Multiepitope Recombinant Protein To Specifically Detect Antibodies against Foot-and-Mouth Disease Virus Serotype O in Swine

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