Identification of thebphC gene formeta-cleavage of aromatic pollutants from a metagenomic library derived from lake waters

Moon, Mi-Sook; Lee, Dong Hun; Kim, Chi-Kyung

doi:10.1007/bf02933064

Cited by 1 publication

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“…The halophile genomic DNAs were prepared from the brine in the Dagong Ancient Brine Well using methods originally described by Moon with modifications (Moon et al ., 2004). Briefly, 100‐mL samples were centrifuged at 14 000 g and 4 °C for 10 min, and the slurry was resuspended with 5 mL phosphate‐buffered saline (pH 7.5) centrifuged at 5 g for 2 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Screening a novel Na⁺/H⁺antiporter gene from a metagenomic library of halophiles colonizing in the Dagong Ancient Brine Well in China

Xiang

Zhang

Lin

et al. 2010

FEMS Microbiology Letters

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Metagenomic DNA libraries constructed from the Dagong Ancient Brine Well were screened for genes with Na(+)/H(+) antiporter activity on the antiporter-deficient Escherichia coli KNabc strain. One clone with a stable Na(+)-resistant phenotype was obtained and its Na(+)/H(+) antiporter gene was sequenced and designated as m-nha. The deduced amino acid sequence of M-Nha protein consists of 523 residues with a calculated molecular weight of 58 147 Da and a pI of 5.50, which is homologous with NhaH from Halobacillus dabanensis D-8(T) (92%) and Halobacillus aidingensis AD-6(T) (86%), and with Nhe2 from Bacillus sp. NRRL B-14911 (64%). It had a hydropathy profile with 10 putative transmembrane domains and a long carboxyl terminal hydrophilic tail of 140 amino acid residues, similar to Nhap from Synechocystis sp. and Aphanothece halophytica, as well as NhaG from Bacillus subtilis. The m-nha gene in the antiporter-negative mutant E. coli KNabc conferred resistance to Na(+) and the ability to grow under alkaline conditions. The difference in amino acid sequence and the putative secondary structure suggested that the m-nha isolated from the Dagong Ancient Brine Well in this study was a novel Na(+)/H(+) antiporter gene.

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Section: Methodsmentioning

confidence: 99%

Screening a novel Na⁺/H⁺antiporter gene from a metagenomic library of halophiles colonizing in the Dagong Ancient Brine Well in China

Xiang

Zhang

Lin

et al. 2010

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

Identification of thebphC gene formeta-cleavage of aromatic pollutants from a metagenomic library derived from lake waters

Cited by 1 publication

References 26 publications

Screening a novel Na⁺/H⁺antiporter gene from a metagenomic library of halophiles colonizing in the Dagong Ancient Brine Well in China

Screening a novel Na⁺/H⁺antiporter gene from a metagenomic library of halophiles colonizing in the Dagong Ancient Brine Well in China

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Identification of thebphC gene formeta-cleavage of aromatic pollutants from a metagenomic library derived from lake waters

Cited by 1 publication

References 26 publications

Screening a novel Na+/H+antiporter gene from a metagenomic library of halophiles colonizing in the Dagong Ancient Brine Well in China

Screening a novel Na+/H+antiporter gene from a metagenomic library of halophiles colonizing in the Dagong Ancient Brine Well in China

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Screening a novel Na⁺/H⁺antiporter gene from a metagenomic library of halophiles colonizing in the Dagong Ancient Brine Well in China

Screening a novel Na⁺/H⁺antiporter gene from a metagenomic library of halophiles colonizing in the Dagong Ancient Brine Well in China