Identification of the very early transcribed baculovirus gene PE-38

Krappa, R; Knebel-Mörsdorf, Dagmar

doi:10.1128/jvi.65.2.805-812.1991

Cited by 97 publications

(73 citation statements)

References 53 publications

(47 reference statements)

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“…An upstream activating region. An examination of the AcMNPV genome demonstrated that the CNE constitutes the central part of a symmetrically arranged region located between the coding sequences of the divergently transcribed immediate early genes ie2 and pe38 [62] ( Figure 1B) ( Figure S4). Both ie2 and pe38 are transcriptional transactivators, participating in many processes, among which DNA replication (ie2 and pe38) and cell cycle arrest (ie2) [22].…”

Section: The Acmnpv Genome Is the Most Appropriate Model To Investigamentioning

confidence: 99%

Identification of a Conserved Non-Protein-Coding Genomic Element that Plays an Essential Role in Alphabaculovirus Pathogenesis

Kikhno

2014

PLoS ONE

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Highly homologous sequences 154–157 bp in length grouped under the name of “conserved non-protein-coding element” (CNE) were revealed in all of the sequenced genomes of baculoviruses belonging to the genus Alphabaculovirus. A CNE alignment led to the detection of a set of highly conserved nucleotide clusters that occupy strictly conserved positions in the CNE sequence. The significant length of the CNE and conservation of both its length and cluster architecture were identified as a combination of characteristics that make this CNE different from known viral non-coding functional sequences. The essential role of the CNE in the Alphabaculovirus life cycle was demonstrated through the use of a CNE-knockout Autographa californica multiple nucleopolyhedrovirus (AcMNPV) bacmid. It was shown that the essential function of the CNE was not mediated by the presumed expression activities of the protein- and non-protein-coding genes that overlap the AcMNPV CNE. On the basis of the presented data, the AcMNPV CNE was categorized as a complex-structured, polyfunctional genomic element involved in an essential DNA transaction that is associated with an undefined function of the baculovirus genome.

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Section: The Acmnpv Genome Is the Most Appropriate Model To Investigamentioning

confidence: 99%

Identification of a Conserved Non-Protein-Coding Genomic Element that Plays an Essential Role in Alphabaculovirus Pathogenesis

Kikhno

2014

PLoS ONE

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“…Furthermore, IE1 is able to inhibit expression of the ie2 and ie0 promoters (7,21). The putative regulatory functions of other early viral genes like pe38 (23), me53 (19), and cg30 (39) are based on sequence analysis. The amino acid sequence of the protein encoded by the pe38 gene contains putative DNA binding and dimerization domains (23).…”

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confidence: 99%

Expression of PE38 and IE2, viral members of the C3HC4 finger family, during baculovirus infection: PE38 and IE2 localize to distinct nuclear regions

Krappa

Roncarati

Knebel-Mörsdorf

1995

J Virol

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The pe38 gene of Autographa californica nuclear polyhedrosis virus represents one of the major early transcripts after viral infection. The function of the pe38 protein, which contains a C 3 HC 4 zinc finger motif, is still not understood. We have raised polyclonal antiserum against the pe38 protein, PE38, produced in bacteria to investigate pe38 expression in the course of infection. A ϳ38-kDa polypeptide is first detectable at 2 h postinfection and decreases rapidly after 24 h. During the late phases of infection, a smaller protein of ϳ20 kDa which cross-reacts with the PE38-specific antiserum is visible at a constant level until 120 h postinfection. Since the pe38 gene shares a divergent promoter unit with the ie2 gene (formerly IEN), we have compared the expressions of the two genes. Polyclonal antibodies were raised against the bacterially expressed ie2 protein.The temporal expression pattern of the ϳ49-kDa ie2 protein is comparable to that of the ϳ38-kDa pe38 protein. Furthermore, both proteins are present in the nuclear fraction of A. californica nuclear polyhedrosis virus-infected Spodoptera frugiperda cells, but the ϳ38-kDa pe38 protein is also detectable in the cytoplasm while the smaller protein of ϳ20 kDa is exclusively present in the cytoplasmic fraction. Immunofluorescence analysis reveals that PE38 and IE2 localize to distinct regions within the nucleus mainly detected after transfection of pe38-and ie2-expressing constructs.

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“…The cells were scanned under a confocal microscope. tion start site (Blissard & Rohrmann, 1989;Chisholm & Henner, 1988;Guarino & Summers, 1986;Guarino & Summers, 1987;Krappa & Kenbel, 1991). The promoters of most late and very late genes have typically TAAG motifs and their transcriptions are mediated by alphaamanitin-resistant RNA polymerase (Blissard & Rohrmann, 1990;Morris & Miller, 1994).…”

Section: Discussionmentioning

confidence: 99%

Expression and immunocytochemical analysis of Autographa californica nucleopolyhedrovirus (AcMNPV) orf74 gene

Guo

2006

Insect Science

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Autographa californica nucleopolyhedrovirus orf74 (Ac74) is located between 62 311 and 63 108bp in the AcMNPV genome, which encodes 265 amino acid residues with a predicted 31 kDa molecular weight. The homologues of Ac74 were searched using BLASTP in protein databases, GenBank/EMBL and SWISS‐PROT. The result revealed that deduced Ac74 protein was homologous to the predicted products from 10 lepidoptera NPV ORFs. The multiple sequence alignments of Ac74 and its 10 homologues manifested only one amino acid residue was completely conserved. The transcript analysis revealed that the transcript of Ac74 was detected from 24–72 hours post‐infection (hpi). The product of Ac74 was detected at 24 hpi and lasted until 72 hpi by Western blot using anti‐Ac74 antiserum, consistent with reverse transcriptase polymerase chain reaction results. These results suggested Ac74 was expressed during the later stages of infection. The product of Ac74 was 31 kDa in size, consistent with predicted molecular weight. The subcellular localization of Ac74 proteins manifested Ac74 protein in the cytoplasm, and was hardly present in the nucleus at 24 hpi. The fluorescence was also observed in polyhedra, except cytoplasm at 72 hpi. Together, Ac74 is a functional protein with 3 1kDa molecular weight and is located in the cytoplasm and the polyhedra.

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Identification of the very early transcribed baculovirus gene PE-38

Cited by 97 publications

References 53 publications

Identification of a Conserved Non-Protein-Coding Genomic Element that Plays an Essential Role in Alphabaculovirus Pathogenesis

Identification of a Conserved Non-Protein-Coding Genomic Element that Plays an Essential Role in Alphabaculovirus Pathogenesis

Expression of PE38 and IE2, viral members of the C3HC4 finger family, during baculovirus infection: PE38 and IE2 localize to distinct nuclear regions

Expression and immunocytochemical analysis of Autographa californica nucleopolyhedrovirus (AcMNPV) orf74 gene

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