Identification of the translocatable element IS1 in a molecular chimera constructed with plasmid pBR322 DNA into which a bacteriophage MS2 DNA copy was inserted by the poly(dA)·Poly(dT) linker method

Devos, René; Contreras, Roland; Emmelo, John van; Fiers, Walter

doi:10.1016/0022-2836(79)90296-1

Cited by 26 publications

(3 citation statements)

References 11 publications

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“…The latter was tentatively identified as the translocatable element IS1 on the basis of both size and the presence of one Pst I and three Hinf I restriction sites at appropriate positions relative to one another (Ohtsubo and Ohtsubo, 1978;Johnsrud, 1979). Two similar observations have been made previously in our laboratory using the same protocol for cloning (insertion with poly(dA).poly(dT) tails into the Pst I site of pBR322): an IS1 sequence was found in MS2 clones (Devos et al, 1979a(Devos et al, , 1979b and also in satellite tobacco necrosis virus clones (J. van Emmelo et al, manuscript in preparation).…”

Section: Resultssupporting

confidence: 70%

Complete structure of the hemagglutinin gene from the human influenza A/Victoria/3/75 (H3N2) strain as determined from cloned DNA

Jou

Verhoeyen

Devos

et al. 1980

Cell

220

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SummaryThe complete sequence of a hemagglutinin (HA) gene of a recent human influenza A strain, A/Victoria/3/75, is 1768 nucleotides long and contains the information for 567 amino acids. It codes for a signal peptide of 16 amino acids, the HA1 chain of the mature hemagglutinin of 329 amino acids, a connecting region between HA1 and HA2 consisting of a single arginine residue and the HA2 portion of 221 amino acids. The sequence is compared with the hemagglutinin of two members of other subtypes, the human H2 strain A/Jap/305/57 and the avian Havl strain A/FPV/Rostock/34, and with one of the same H3 subtype, A/Memphis/3/72. To align the HA1 chain of different major subtypes several deletions/insertions of single amino acids must be invoked, but two more extensive differences are found at both ends, one leading to an extension of the amino terminal sequence of HA1 and the other (four residues) occurring in the region processed away between HA1 and HA2. Comparison of the HA1 of two H3 strains suggests that drift probably depends on single base mutations, some of which change antigenic determinants. The HA2 region, which apparently is not involved in the immune response, is highly conserved even between different subtypes, and single base substitutions account for all the observed diversity. A hydrophobic segment of 24 residues is present in the same position close to the carboxyl terminus of HA2 in both Victoria and FPV, and presumably functions in implantation into the lipid bilayer. The many conserved features not only in HA2 but also in HA1 suggest a rather rigid architecture for the whole hemagglutinin molecule.

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Section: Resultssupporting

confidence: 70%

Complete structure of the hemagglutinin gene from the human influenza A/Victoria/3/75 (H3N2) strain as determined from cloned DNA

Jou

Verhoeyen

Devos

et al. 1980

Cell

220

View full text Add to dashboard Cite

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“…The requirement for transcription in the generation of deletion plasmids is understandable if the activity that leads to deletion formation is catalyzed by transposase. Several workers report that IS5 preferentially transposes into regions of high A+T content, that is, into regions that are easily denaturable (9,11,20,25). Transcription of the plasmids used in this study may provide transient single-strandedness, which facilitates transposasecatalyzed events.…”

Section: Discussionmentioning

confidence: 90%

Recombination in recA cells between direct repeats of insertion element IS1

Braedt¹

1985

J Bacteriol

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The IS1 sequences that flank the Tn9 chloramphenicol acetyltransferase gene as direct repeats recombine after transformation into an Escherichia coli recA strain. The recombination requires the lambda pL promoter on the plasmid. A plasmid that contains mutant IS1 elements does not recombine. These results indicate that this recombination requires an IS1-specific gene product. The recombinational activity of IS1 may resolve transient cointegrates formed during the transposition of IS1. I discuss a possible role for the lambda pL promoter.

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“…Moreover, the creation of MS2 cNDA in vitro was accompanied by artificial attachment of poly (dA) poly (dT) linker to MS2 RNA [24,25], the absence of which makes it unsuitable for the operation of reverse transcriptase. Unlike retroviruses, intermediate DNA-containing structures associated with reproduction of viral RNA have not been described for RNA-containing bacteriophages.…”

Section: Discussionmentioning

confidence: 99%

Interaction of RNA-containing bacteriophages with host cell: MS2-induced mutants of E. coli and the occurrence of DNA-containing derivatives of the bacteriophage MS2

Pererva

Miryuta

2008

Cytol. Genet.

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Sensitive cells of Escherichia coli AB 259 Hfr 3000 infected with RNA-containing phage MS2 produce phage particles and simultaneously continue to divide, thereby segregating sensitive cells capable of sustaining new cycles of infection. Multiplication of phage in sensitive cells gives rise to phage-resistant forms in the progeny of these cells. It is shown that this phenomenon is due not to selection of pre-existing phage-resistant mutants, but is instead the result of interaction between the phage and the cell. Unlike ordinary spontaneous or chemically induced E. coli mutants, MS2-induced phage-resistant cells are genetically unstable forms. In the course of reproduction they segregate new MS2-resistant forms with more highly expressed variations in the region encoded by the sex factors. Cells of the two final forms of MS2-induced mutants also produce a new type of phage. This new type constitutes DNA-containing forms which, however, are neutralized by anti-MS2serum. The segregation of these forms serves to confirm that the genetic substance of the RNA-containing bacteriophage is capable of being expressed as a component of the DNA-containing structure.

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Identification of the translocatable element IS1 in a molecular chimera constructed with plasmid pBR322 DNA into which a bacteriophage MS2 DNA copy was inserted by the poly(dA)·Poly(dT) linker method

Cited by 26 publications

References 11 publications

Complete structure of the hemagglutinin gene from the human influenza A/Victoria/3/75 (H3N2) strain as determined from cloned DNA

Complete structure of the hemagglutinin gene from the human influenza A/Victoria/3/75 (H3N2) strain as determined from cloned DNA

Recombination in recA cells between direct repeats of insertion element IS1

Interaction of RNA-containing bacteriophages with host cell: MS2-induced mutants of E. coli and the occurrence of DNA-containing derivatives of the bacteriophage MS2

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