Identification of the Sex Pheromone of the Oriental Tobacco Budworm, Heliothis assulta(GUENEE)(Lepidoptera:Noctuidae)

Sugie, Hajime; Tatsuki, Sadahiro; Nakagaki, Shiro; Rao, C. Bhashyakarla; Yamamoto, Akira

doi:10.1303/aez.26.151

Cited by 37 publications

(17 citation statements)

References 2 publications

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“…They both use ( Z )-11-hexadecenal (Z11–16: Ald) and ( Z )-9-hexadecenal (Z9–16: Ald) as principal sex pheromones but in different ratios [35]–[37]. Females of H. armigera produce 97% Z11–16: Ald and 3% Z9–16: Ald whereas H. assulta females produce 7% Z11–16: Ald and 93% Z9–16: Ald, ensuring premating-isolation of the two species.…”

Section: Introductionmentioning

confidence: 99%

Peripheral Coding of Sex Pheromone Blends with Reverse Ratios in Two Helicoverpa Species

Hou

Huang

et al. 2013

PLoS ONE

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The relative proportions of components in a pheromone blend play a major role in sexual recognition in moths. Two sympatric species, Helicoverpa armigera and Helicoverpa assulta, use (Z)-11-hexadecenal (Z11–16: Ald) and (Z)-9-hexadecenal (Z9–16: Ald) as essential sex pheromone components but in very different ratios, 97∶3 and 7∶93 respectively. Using wind tunnel tests, single sensillum recording and in vivo calcium imaging, we comparatively studied behavioral responses and physiological activities at the level of antennal sensilla and antennal lobe (AL) in males of the two species to blends of the two pheromone components in different ratios (100∶0, 97∶3, 50∶50, 7∶93, 0∶100). Z11–16: Ald and Z9–16: Ald were recognized by two populations of olfactory sensory neurons (OSNs) in different trichoid sensilla on antennae of both species. The ratios of OSNs responding to Z11–16:Ald and Z9–16:Ald OSNs were 100∶28.9 and 21.9∶100 in H. armigera and H. assulta, respectively. The Z11–16:Ald OSNs in H. armigera exhibited higher sensitivity and efficacy than those in H. assulta, while the Z9–16:Ald OSNs in H. armigera had the same sensitivity but lower efficacy than those in H. assulta. At the dosage of 10 µg, Z11–16: Ald and Z9–16: Ald evoked calcium activity in 8.5% and 3.0% of the AL surface in H. armigera, while 5.4% and 8.6% of AL in H. assulta, respectively. The calcium activities in the AL reflected the peripheral input signals of the binary pheromone mixtures and correlated with the behavioral output. These results demonstrate that the binary pheromone blends were precisely coded by the firing frequency of individual OSNs tuned to Z11–16: Ald or Z9–16: Ald, as well as their population sizes. Such information was then accurately reported to ALs of H. armigera and H. assulta, eventually producing different behaviors.

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Section: Introductionmentioning

confidence: 99%

Peripheral Coding of Sex Pheromone Blends with Reverse Ratios in Two Helicoverpa Species

Hou

Huang

et al. 2013

PLoS ONE

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“…Whereas most of the species rely on cis-11-hexadecenal (Z11-16:AL) as the major pheromone component, H. assulta utilizes this substance as the second component, and cis-9-hexadecenal (Z9-16:AL) as the major one (Sugie et al, 1991;Cork et al, 1992;Park et al, 1994). The optimal ratio between the major and the second principal pheromone component is 20:1 in the Korean population of H. assulta (Cork et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Projections of male-specific receptor neurons in the antennal lobe of the oriental tobacco budworm moth,Helicoverpa assulta: A unique glomerular organization among related species

et al. 2005

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The macroglomerular complex in the primary olfactory center of male moths receives information from numerous pheromone-detecting receptor neurons housed in specific sensilla located on the antennae. We investigated the functional organization of the three glomeruli constituting this complex in Helicoverpa assulta, a unique species among heliothine moths as concerns the composition of the pheromone blend. By tip recordings from the male-specific receptor neurons combined with cobalt-lysine stainings, the axon terminals in the brain were traced and subsequently reconstructed by camera lucida drawings. Some were also reconstructed in a digital form. The results showed that the sensilla could be classified into two functional types. A major category housed two colocalized receptor neurons, one responding to the primary pheromone component cis-9-hexadecenal and the other to the behavioral antagonists cis-9-tetradecenal and cis-9-hexadecenol. Cobalt-lysine applied to this sensillum type consistently resulted in two stained axons, each terminating in one of the two large subunits of the macroglomerular complex: the cumulus or the dorsomedial glomerulus. The second, less frequently appearing sensillum type contained a receptor neuron responding to the second pheromone component, cis-11-hexadecenal. Dye applied to this type resulted in stained axon projections in the ventral glomerulus. In an evolutionary context it is particularly interesting that differences of related heliothine species are reflected in the functional organization of the MGC compartments.

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“…The two species are widely sympatric on the American continent. In the oriental species H. assulta, the major component is Z9-16:AL, whereas Z11-16:AL is the second principal pheromone component (Cork et al 1992;Sugie et al 1991). The two compounds are released in a relative amount of about 20:1 in the Korean population (Cork et al 1992).…”

Section: Introductionmentioning

confidence: 99%

The significance of major pheromone components and interspecific signals as expressed by receptor neurons in the oriental tobacco budworm moth, Helicoverpa assulta

Berg¹,

Mustaparta²

1995

J Comp Physiol A

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Receptor neuron specificities for intra-and interspecific chemical signals were determined in males of Helicoverpa assulta, by testing single neurons for twelve heliothine produced compounds and two chemical analogues. Three types of receptor neurons were identified in the male specific sensilla trichodea type 1. 1. One large group of neurons (29 out of 63) was tuned to the major pheromone component (Z)-9-hexadecenal, in contrast to results obtained previously in a related species, where the information from this compound seems to be mediated via neurons tuned to (Z)-9-tetradecenal. 2. Another group of neurons (28/63) was tuned to (Z)-9-tetradecenal which is not produced by the conspecific females. These neurons and those tuned to the major pheromone component, always appearing together, are probably located in the same sensillum. Their large number suggests that (Z)-9-tetradecenal mediates an important message in this species, probably causing interspecific interruption. 3. The third group of neurons (6/63) was tuned to the second principal pheromone component (Z)-ll-hexadecenal. These neurons showed similar specificities as the corresponding type of neurons in related species, indicating a conservation of their membrane receptors through evolution. In contrast, the (Z)-9-tetradecenal receptor neurons in H. assulta showed a different specificity than their counterparts in the related species, suggesting that their receptor proteins have evolved differently.Abbreviations Z9-16:AL (Z)-9-hexadecenal Zll-16.AL (Z)-I 1-hexadecenal Z9-14:AL (Z)-9-tetradecenal ZT-16:AL (Z)-7-hexadecenal -16:AL hexadecanal 14:AL tetradecanal 9 Z9-16:AC (Z)-9-tetradecenyl acetate 9 Zll-16:AC (Z)-I 1-hexadecenyl acetate ZT-16:A C (Z)-7-hexadecenyl acetate 16:AC hexadecanyl acetate 9 Z9-16.'OH (Z)-9-hexadecenol 9 Zll-16.OH (Z)-I 1-hexadecenol 16:OH hexadecanol 9

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Identification of the Sex Pheromone of the Oriental Tobacco Budworm, Heliothis assulta(GUENEE)(Lepidoptera:Noctuidae)

Cited by 37 publications

References 2 publications

Peripheral Coding of Sex Pheromone Blends with Reverse Ratios in Two Helicoverpa Species

Peripheral Coding of Sex Pheromone Blends with Reverse Ratios in Two Helicoverpa Species

Projections of male-specific receptor neurons in the antennal lobe of the oriental tobacco budworm moth,Helicoverpa assulta: A unique glomerular organization among related species

The significance of major pheromone components and interspecific signals as expressed by receptor neurons in the oriental tobacco budworm moth, Helicoverpa assulta

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