Identification of the RGG Box Motif in Shadoo: RNA-Binding and Signaling Roles?

Corley, Susan M.; Gready, Jill E.

doi:10.4137/bbi.s1075

Cited by 34 publications

(39 citation statements)

References 94 publications

(75 reference statements)

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“…2a) indicate that the reduced transcriptional repression of RepSAA mutants compared with their non-mutated counterparts is not even a consequence of defects in nuclear localization. The relevance of the RGG sequence in the transcriptional repression activity, particularly evident in the Rep-130 context, is congruent with the role exerted by proteins containing RGG motifs (Alex & Lee, 2005;Corley & Gready, 2008). Interestingly, a lower repression of the AL1 gene transcription in vitro was reported for an RS-R125 mutant of TGMV Rep (where R125 of TGMV Rep corresponds to R124 of TYLCSV Rep) (Orozco et al, 2000).…”

Section: Role Of An Rgg Sequence In Tylcsv Repsupporting

confidence: 50%

“…At amino acidic position 124-126 a highly conserved RGG triplet was noted, which was present in most sequences retrieved except for some New World species. Proteins with RGGbox motifs are involved in transcriptional repression and in binding and processing of RNA; these motifs are also typical of proteins localized in the nucleus/nucleolus (Burd & Dreyfuss, 1994;Godin & Varani, 2007;Corley & Gready, 2008). Since transcriptional repression, a process expected to take place in the nucleus, is fundamental for the resistance conferred by Rep-210 and Rep-130, we focused on this RGG sequence and tested its role in the subcellular localization and in the inhibition of the C1-gene transcription and TYLCSV replication by using truncated Rep mutants with the RGG sequence substituted by SAA (Rep-210SAA and Rep-130SAA).…”

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confidence: 99%

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An RGG sequence in the replication-associated protein (Rep) of Tomato yellow leaf curl Sardinia virus is involved in transcriptional repression and severely impacts resistance in Rep-expressing plants

Sardo¹,

Lucioli²,

Tavazza³

et al. 2010

Journal of General Virology

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Truncated versions of the replication-associated protein (Rep) of Tomato yellow leaf curl Sardinia virus (TYLCSV) can interfere with various viral functions and the N-terminal 130 aa are sufficient for strongly inhibiting C1-gene transcription and virus replication and confer resistance in transgenic plants. In this work, we analysed the relevance of an RGG sequence at aa 124-126, highly conserved in begomoviruses, in these inhibitory functions as well as in the subcellular localization of Rep. Although no role of this RGG sequence was detected by cell fractionation and immunogold labelling in Rep localization, this sequence appears relevant for the transcriptional control of the C1-gene and for the inhibition of viral replication and dramatically impacts resistance in transgenic plants. These results are discussed in the context of the model of Repmediated resistance against TYLCSV.Begomoviruses (family Geminiviridae) are ssDNA viruses infecting a wide range of crops and causing severe diseases worldwide. Plants resistant to begomoviruses have been obtained by genetic engineering, and ORF C1/AL1/L1 encoding the replication-associated protein (Rep) has been successfully used for this purpose (Vanderschuren et al., 2007;Prins et al., 2008). Rep is a 40-41 kDa multitask protein necessary for virus replication, specifically cleaving the viral genome within a conserved stem-loop structure (Heyraud-Nitschke et al., 1995; Laufs et al., 1995). During virus multiplication, Rep binds to upstream untranslated viral sequences, known as iterons, repressing its own expression (Sunter et al., 1993;Eagle et al., 1994; HanleyBowdoin et al., 1999). Rep forms homo-oligomers of various aggregation orders (Orozco et al., 1997(Orozco et al., , 2000 through central oligomerization domains (Orozco et al., 2000;Choudhury et al., 2006), which in the case of Tomato yellow leaf curl Sardinia virus (TYLCSV) lies between aa 120 and 180 Clérot & Bernardi, 2006). Begomoviruses multiply in the nuclei of infected cells (Hanley-Bowdoin et al., 1999) and perturb the host cell cycle to favour their replication (Ascencio-Ibáñez et al., 2008). In this context, Rep was shown to interact with cell cycle factors such as proliferating cell nuclear antigen or homologues of the retinoblastoma family (pRBR) (Xie et al., 1995;Kong et al., 2000).Nicotiana benthamiana and tomato plants expressing a truncated TYLCSV Rep (Rep-210) are resistant to TYLCSV (Noris et al., 1996;Brunetti et al., 1997) by using a mechanism involving the transcriptional inhibition of the C1-gene promoter and/or the formation of dysfunctional oligomers between Rep-210 and the viral Rep (Brunetti et al., 2001;Lucioli et al., 2003). Further work showed that Rep-130 is the minimal sequence tightly inhibiting C1-gene transcription and virus replication in protoplasts, conferring high levels of virus resistance in transgenic plants (Lucioli et al., , 2008. Since a further deletion of 10 aa (121-RSARGGQQTA-130) consistently reduced the inhibitory activity , we decided to investigate thi...

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Section: Role Of An Rgg Sequence In Tylcsv Repsupporting

confidence: 50%

mentioning

confidence: 99%

An RGG sequence in the replication-associated protein (Rep) of Tomato yellow leaf curl Sardinia virus is involved in transcriptional repression and severely impacts resistance in Rep-expressing plants

Sardo¹,

Lucioli²,

Tavazza³

et al. 2010

Journal of General Virology

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“…48 In general, the RGG box domain is characterized as having two or three RGG repeats, with five to nine residues separating the repeats, and has been identified in 44 human proteins. 49, 50 FMRP has been shown to use its RGG box domain to bind mRNA targets that form G quadruplex structures (Table 1). 19, 24, 27–29, 33, 34, 51, 52 However, with the exception of the 5′-UTRs from microtubule associated protein 1B (MAP1B) mRNA, 27, 40 the protein phosphatase 2A catalytic subunit mRNA, 39 and the hASH1 mRNA 5′-UTR, 48 characterization of the FMRP RGG box binding G quadruplex structures has focused on coding region and 3′-UTR locations.…”

Section: Introductionmentioning

confidence: 99%

Fragile X mental retardation protein recognizes a G quadruplex structure within the survival motor neuron domain containing 1 mRNA 5′-UTR

et al. 2017

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G quadruplex structures have been predicted by bioinformatics to form in the 5′- and 3′-untranslated regions (UTRs) of several thousand mature mRNAs and are believed to play a role in translation regulation. Elucidation of these roles has primarily been focused on the 3′-UTR, with limited focus on characterizing the G quadruplex structures and functions in the 5′-UTR. Investigation of the affinity and specificity of RNA binding proteins for 5′-UTR G quadruplexes and the resulting regulatory effects have also been limited. Among the mRNAs predicted to form a G quadruplex structure within the 5′-UTR is the survival motor neuron domain containing 1 (SMNDC1) mRNA, encoding a protein that is critical to the spliceosome. Additionally, this mRNA has been identified as a potential target of the fragile X mental retardation protein (FMRP), whose loss of expression leads to fragile X syndrome. FMRP is an RNA binding protein involved in translation regulation that has been shown to bind mRNA targets that form G quadruplex structures. In this study we have used biophysical methods to investigate G quadruplex formation in the 5′-UTR of SMNDC1 mRNA and analyzed its interactions with FMRP. Our results show that SMNDC1 mRNA 5′-UTR forms an intramolecular, parallel G quadruplex structure comprised of three G quartet planes, which is bound specifically by FMRP both in vitro and in mouse brain lysates. These findings suggest a model by which FMRP might regulate the translation of a subset of its mRNA targets by recognizing the G quadruplex structure present in their 5′-UTR, and affecting their accessibility by the protein synthesis machinery.

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“…This suggests that the N-terminus of PrP C and Shadoo possesses a conserved physiological activity. Recently, published data have revealed that the Shadoo N-terminal domain (and especially the RGG-box region) can also bind nucleic acids, especially RNAs (Lau and Westaway Prion 2010 conference, Salzburg; [71]) suggesting that this domain is most certainly involved in HIV-1 inhibition. The role of Shadoo in the cell defense against viral injury should be investigated in the future.…”

Section: Discussionmentioning

confidence: 99%

Functional mechanisms of the cellular prion protein (PrPC) associated anti-HIV-1 properties

Alais

Soto-Rifo

Balter

et al. 2011

Cell. Mol. Life Sci.

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The cellular prion protein PrP(C)/CD230 is a GPI-anchor protein highly expressed in cells from the nervous and immune systems and well conserved among vertebrates. In the last decade, several studies suggested that PrP(C) displays antiviral properties by restricting the replication of different viruses, and in particular retroviruses such as murine leukemia virus (MuLV) and the human immunodeficiency virus type 1 (HIV-1). In this context, we previously showed that PrP(C) displays important similarities with the HIV-1 nucleocapsid protein and found that PrP(C) expression in a human cell line strongly reduced HIV-1 expression and virus production. Using different PrP(C) mutants, we report here that the anti-HIV-1 properties are mostly associated with the amino-terminal 24-KRPKP-28 basic domain. In agreement with its reported RNA chaperone activity, we found that PrP(C) binds to the viral genomic RNA of HIV-1 and negatively affects its translation. Using a combination of biochemical and cell imaging strategies, we found that PrP(C) colocalizes with the virus assembly machinery at the plasma membrane and at the virological synapse in infected T cells. Depletion of PrP(C) in infected T cells and microglial cells favors HIV-1 replication, confirming its negative impact on the HIV-1 life cycle.

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Identification of the RGG Box Motif in Shadoo: RNA-Binding and Signaling Roles?

Cited by 34 publications

References 94 publications

An RGG sequence in the replication-associated protein (Rep) of Tomato yellow leaf curl Sardinia virus is involved in transcriptional repression and severely impacts resistance in Rep-expressing plants

An RGG sequence in the replication-associated protein (Rep) of Tomato yellow leaf curl Sardinia virus is involved in transcriptional repression and severely impacts resistance in Rep-expressing plants

Fragile X mental retardation protein recognizes a G quadruplex structure within the survival motor neuron domain containing 1 mRNA 5′-UTR

Functional mechanisms of the cellular prion protein (PrPC) associated anti-HIV-1 properties

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