Identification of the retinoblastoma (Rb) gene and expression in response to environmental stressors in the intertidal copepod Tigriopus japonicus

Developmental retardation, reduced fecundity, and modulated expression of the defensome in the intertidal copepod Tigriopus japonicus exposed to BDE-47 and PFOS

“…Likewise, the mRNA of retinoblastoma (TJ-Rb) gene was time dependently upregulated over 12 h (P < 0.001) and 48 h (P < 0.01) in response to BDE-47 and PFOS exposure respectively. Recently, the Tj-Rb gene was identified from transcriptomic studies of the response to environmental stressors in T. japonicus (Kim et al, 2015a). Upregulation of Tj-Rb in response to BDE-47 and PFOS exposure in T. japonicus indicates that Rb can be a useful indicator of the deleterious effects of BDE-47 and PFOS exposure.…”

“…Upregulation of Tj-Rb in response to BDE-47 and PFOS exposure in T. japonicus indicates that Rb can be a useful indicator of the deleterious effects of BDE-47 and PFOS exposure. In addition, the significant induction (P < 0.05) of the Tj-Rb gene in response to UV radiation suggests that Tj-Rb is a promising molecular marker for various environmental stressors (Kim et al, 2015a), as Rb is a key factor associated with cell growth, differentiation, cell cycle regulation, and apoptosis (Fan and Steer, 1999). In T. japonicus, a cellular proliferating gene, Tj-PCNA, was also induced by exposure to BDE-47 or PFOS.…”

Gut Microbiome Alteration of Cold-adapated Antarctic copepod Tigriopus kingsejongensis with Temperature Changes and Developmental Stages

“…After 2-48 h of incubation, their stool was collected and used as samples for analyzing the stool microbial community. Seawater was used in the experiment after ltration and sterilization following the methods of Lee et al [21]. Adult copepods were transferred to three different temperatures, namely 2°C, 8°C, and 15°C, after washing with sterilized seawater.…”