Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing

Mu, Yanjun; Gong, Wenping; Qie, Yanmin; Liu, Xueqing; Li, Linzhi; Sun, Nina; Liu, Wei; Guo, Jun; Han, Ran; Yu, Ziyang; Xiao, Luning; Su, Fuyu; Zhang, Wenjing; Jiangchun, Wang; Han, Guohao; Ma, Pengtao

doi:10.3389/fpls.2022.1005627

Cited by 13 publications

(10 citation statements)

References 48 publications

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“…The strong LD makes it challenging to discover the causal genes of the association, which could be a single or multiple resistance genes. Indeed, many candidate Pm genes have been described in this genomic interval, including Pm51 (57), Pm63 (60), PmKN0816 (62), PmLS5082 (63), PmQ (64) and pmYN99102 (66). Accessions that likely contained the causal gene were predominantly from Iran and the USA.…”

Section: Resultsmentioning

confidence: 99%

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A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance

Leber

Heuberger

Widrig

et al. 2023

Preprint

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Wheat breeding for disease resistance relies on the availability and use of diverse genetic resources. More than 800,000 wheat accessions are globally conserved in gene banks, but they are mostly uncharacterized for the presence of resistance genes and their potential for agriculture. Based on the selective reduction of previously assembled collections for allele mining, we assembled a trait-customized panel of 755 geographically diverse bread wheat accessions with a focus on landraces, called the LandracePLUS panel. Population structure analysis of this panel based on the TaBW35K SNP array revealed an increased genetic diversity compared to high-quality sequenced wheat accessions and 632 landraces of an earlier study. The additional diversity mostly originated from Turkish, Iranian and Pakistani landraces. We characterized the LandracePLUS panel for resistance to ten diverse isolates of the fungal pathogen powdery mildew. Performing genome-wide association studies and dividing the panel further by a targeted sub-setting approach for accessions with unique resistance patterns and distinct geographical origin, we detected several known and already cloned genes, including thePm2agene. In addition, we identified 17 putatively novel powdery mildew resistance loci that represent useful sources for resistance breeding and for research on the mildew-wheat pathosystem. Our study shows the value of assembling trait-customized collections and utilizing a diverse range of pathogen races, to detect novel loci that can be integrated in breeding programs. It further highlights the importance of integrating landraces of different geographical origin into future diversity studies.

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Section: Resultsmentioning

confidence: 99%

“…This introgression is potentially present in the LandracePLUS panel. Many candidate Pm genes have already been described in this genomic interval, including Pm51 (Zhan et al 2014), Pm63 (Tan et al 2019), PmKN0816 (Wang et al 2021), PmLS5082 (Wu et al 2022), PmQ (Li et al 2020b), pmYN99102 (Mu et al 2022) and PmCG15-009 (Zhang et al 2023).…”

Section: Resultsmentioning

confidence: 99%

A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance

Leber

Heuberger

Widrig

et al. 2023

Preprint

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show abstract

“…The balance of resistance and yield is the main concern for breeders when using a resistance gene in wheat breeding programs, but often, disease resistance is at the expense of some agronomic traits and reduces plant adaptation ( Deng et al., 2017 ; Han et al., 2022 ; Mu et al., 2022 ). For example, mildew resistance locus O ( MLO ), is a durable and broad-spectrum resistance to powdery mildew in various plant species including common wheat, however, it also leads to growth penalties and yield losses, thereby limiting its widespread use in wheat breeding ( Li et al., 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew

Liu

Han²,

Gu³

et al. 2022

Front. Plant Sci.

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Developing effective and durable host plant resistance is crucial for controlling powdery mildew, a devastating disease caused by Blumeria graminis f. sp. tritici (Bgt). In the present study, we dissected the genetic basis of the adult plant resistance to powdery mildew using a recombinant inbred line (RIL) composed of 176 F9 RILs population derived from a cross between PuBing 3228 (P3228) and susceptible cultivar Gao 8901. P3228 exhibits stable adult-plant resistance to powdery mildew in the field over consecutive years. We identified two QTLs on chromosomes 7DS (QPm.cas-7D) and 1AL (QPm.cas-1A) contributed by P3228, and one QTL on 3DS (QPm.cas-3D) contributed by Gao 8901, which could explain 65.44%, 3.45%, and 2.18% of the phenotypic variances, respectively. By analyzing the annotated genes in the 1.168 Mb physical interval of the major QTL QPm.cas-7D, we locked a previously cloned adult-plant resistance gene Pm38 that was most probably the candidate gene of QPm.cas-7D. Sequence alignment analysis revealed that the candidate gene of QPm.cas-7D in P3228 was identical to the reported Pm38 sequence. Two haplotypes QPm-7D-R and QPm-7D-S were identified in the whole Pm38 genomic regions between P3228 and Gao 8901. To apply QPm.cas-7D in wheat breeding, we developed a kompetitive allele-specific PCR (KASP) marker Kasp5249 that is closely linked with these haplotypes. It is worth mentioning that the QPm-7D-R haplotype significantly decreased TKW and underwent negative selection for higher yields in China wheat breeding. In this study, we identified a major QTL QPm.cas-7D and revealed the relationship between its resistance and yield, which could be beneficial for further applications in wheat disease resistance and high-yield breeding.

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“…Pm64 was derived from wild emmer wheat and located on the interval of 695.4-710.3 Mb (Zhang et al 2019). PmKN0816 (700.4-722.1 Mb), PmLS5082, PmCG15-009 and the recessive gene pmYN99102 were all derived from wheat breeding lines (Mu et al 2022;Wang et al 2021;Wu et al 2022;Zhang et al 2023).…”

Section: Discussionmentioning

confidence: 99%

Characterization and identification of the powdery mildew resistance gene in wheat breeding line Jimai 809

Zhao,

Han,

Qie

et al. 2023

Preprint

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Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a severe disease that seriously affects the yield and quality of wheat. Using resistant cultivars in the production is the preferred strategy to control this disease. In the current study, the wheat breeding line Jimai 809 from Shandong Province of China showed high resistance to powdery mildew at the whole growth stage. To dissect the genetic basis for this resistance, Jimai 809 was crossed with the susceptible wheat cultivar Junda 159 to produce F1, F2 and F2:3 populations. Genetic analysis showed that a single dominant gene, temporarily named PmJM809, conferred the resistance to the Bgt isolate E09. Further molecular markers analysis showed that PmJM809 was located on chromosome 2BL and flanked by the markers CISSR02g-1 and CIT02g-13 with the genetic distances 0.4 and 0.8 cM, respectively, corresponding to a physical interval of 704.12-708.24 Mb referred to Chinese Spring reference genome sequence v1.1. PmJM809 differed from the Pm genes previously reported on chromosome arm 2BL in origin, resistance spectrum, physical position and genetic diversity of the interval, and hence was most likely a novel Pm gene or new allele of these reported Pm genes. To analyze and screen the candidate genes of PmJM809, six genes related to disease resistance in the candidate interval of PmJM809 were evaluated their expression pattern using an additional set of wheat samples and time-course analysis post-inoculation of Bgt isolate, and among them, three genes were locked as the key candidate genes. To efficiently transfer and track PmJM809 in different wheat genetic backgrounds, 13 of 19 closely linked/co-segregated markers were selected to be suitable for marker-assisted selection.

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Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing

Cited by 13 publications

References 48 publications

A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance

A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance

Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew

Characterization and identification of the powdery mildew resistance gene in wheat breeding line Jimai 809

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