Identification of the post-translational modifications of the core-specific lectin. The core-specific lectin contains hydroxyproline, hydroxylysine, and glucosylgalactosylhydroxylysine residues.

Colley, Karen J.; Baenziger, Jacques U.

doi:10.1016/s0021-9258(18)61111-1

Cited by 52 publications

(7 citation statements)

References 23 publications

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“…To determine the rhMBL pI, 2D-PAGE and isoelectric focusing were performed. The result showed the presence of two isoforms of rhMBL, with pI in a range from 5.7 to 5.9, which is close to the pI reported for MBL purified from rat liver [50] (figure 2). Jensen et al (2007) observed, among those modifications, that the only detectable difference between the purified plasma-derived human MBL and the rhMBL was the content of hydroxyprolines (Hyp) (mostly) and glycosylated hydroxylysines (Hyl) (figure 3) [51].…”

Section: Resultssupporting

confidence: 84%

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

Lima

Oliveira

Silva

et al. 2022

Methods Appl. Fluoresc.

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Quantum dots (QDs) have stood out as nanotools for glycobiology due to their photostability and ability to be combined with lectins. Mannose-binding lectin (MBL) is involved in the innate immune system and plays important roles in the activation of the complement cascade, opsonization, and elimination of apoptotic and microbial cells. Herein, adsorption and covalent coupling strategies were evaluated to conjugate QDs to a recombinant human MBL (rhMBL). The most efficient nanoprobe was selected by evaluating the conjugate ability to label Candida albicans yeasts by flow cytometry. The QDs-rhMBL conjugate obtained by adsorption at pH 6.0 was the most efficient, labeling ca. 100% of cells with the highest median fluorescence intensity. The conjugation was also supported by Fourier transform infrared spectroscopy, zeta potential, and size analyses. C. albicans labeling was calcium-dependent; 12% and <1% of cells were labeled in buffers without calcium and containing EDTA, respectively. The conjugate promoted specific labeling (based on cluster effect) since, after inhibition with mannan, there was a reduction of 80% in cell labeling, which did not occur with methyl-α-D-mannopyranoside monosaccharide. Conjugates maintained colloidal stability, bright fluorescence, and biological activity for at least 8 months. Therefore, QDs-rhMBL conjugates are promising nanotools to elucidate the roles of MBL in biological processes.

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Section: Resultssupporting

confidence: 84%

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

Lima

Oliveira

Silva

et al. 2022

Methods Appl. Fluoresc.

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“…The subunit molecular weights are different from Clq, acetylcholinesterase, and the core-specific lectin. Unlike the latter protein, CP4 contains asparagine-linked carbohydrate (Colley & Baenziger, 1987a). The functional role of the collagenous domain of SP-35 is not yet known.…”

Section: Discussionmentioning

confidence: 99%

CP4: a pneumocyte-derived collagenous surfactant-associated protein. Evidence for heterogeneity of collagenous surfactant proteins

Persson¹,

Rust²,

Chang³

et al. 1988

Biochemistry

104

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Type II pneumocytes secrete pulmonary surfactant and are known to synthesize SP-35, a collagenous surfactant-associated protein. Freshly isolated type II cells also synthesize other bacterial collagenase-sensitive and hydroxyproline-containing proteins, including a glycoprotein designated CP4. CP4 was isolated from rat pneumocyte culture medium by immune precipitation with polyclonal antibodies to rat surfactant proteins or by DEAE chromatography and reverse-phase or gel permeation HPLC. CP4 did not cross-react with polyclonal antibodies to SP-35 and was completely resolved from SP-35 by SDS-PAGE (Mr 43K reduced) or isoelectric focusing. Unlike SP-35, which consists of acidic isoforms assembled as disulfide-bonded dimers and multimers, CP4 was secreted as basic isoforms assembled as disulfide-bonded trimers. Differences in primary structure were demonstrated by CNBr and V8 protease peptide mapping. The secretion of both proteins was inhibited by 2,2'-dipyridyl, an inhibitor of posttranslational prolyl and lysyl hydroxylation and collagen triple helix formation. CP4 was isolated from EDTA extracts of rat surfactant. These studies provide evidence for the heterogeneity of pneumocyte-derived collagenous surfactant-associated proteins.

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“…138,139 These and other motifs are beyond the scope of this review, but certainly represent novel linkages and modifications, including Glcb1-3Fuca1-Ser/Thr in EGF modules, 140 Siaa2-3Galb1-4GlcNAcb1-3Fuca1-Ser/Thr in notch, 141 and Glca1-2Galb1-hydroxylysine in collagen 142 and the serum mannose binding lectin. 143,144 In addition to the well known phosphorylation of mannose to give mannose-6phosphate, other glycans may also be phosphorylated and sulfated, as seen in -GlcUAb1-3Gal(AE6-O-sulfate)-b1-3Gal(AE6-O-sulfate)b1-4Xyl and -GlcUA-Gal-Gal-Xyl-(2-O-phosphate)-of the linkage region of proteoglycans. 145,146 Other unusual structures, such as Glcb1-6(Galb1-3)GalNAca1-Ser/Thr and Glcb1-6(Glcb1-3)GalNAca1-Ser/Thr, 147 have been identified in Caenorhabditis elegans where they are abundant, and raise questions whether they might also occur in higher animals.…”

Section: How Many Glycan Determinants Exist?mentioning

confidence: 99%

The repertoire of glycan determinants in the human glycome

2009

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The number of glycan determinants that comprise the human glycome is not known. This uncertainty arises from limited knowledge of the total number of distinct glycans and glycan structures in the human glycome, as well as limited information about the glycan determinants recognized by glycan-binding proteins (GBPs), which include lectins, receptors, toxins, microbial adhesins, antibodies, and enzymes. Available evidence indicates that GBP binding sites may accommodate glycan determinants made up of 2 to 6 linear monosaccharides, together with their potential side chains containing other sugars and modifications, such as sulfation, phosphorylation, and acetylation. Glycosaminoglycans, including heparin and heparan sulfate, comprise repeating disaccharide motifs, where a linear sequence of 5 to 6 monosaccharides may be required for recognition. Based on our current knowledge of the composition of the glycome and the size of GBP binding sites, glycoproteins and glycolipids may contain approximately 3000 glycan determinants with an additional approximately 4000 theoretical pentasaccharide sequences in glycosaminoglycans. These numbers provide an achievable target for new chemical and/or enzymatic syntheses, and raise new challenges for defining the total glycome and the determinants recognized by GBPs.

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Identification of the post-translational modifications of the core-specific lectin. The core-specific lectin contains hydroxyproline, hydroxylysine, and glucosylgalactosylhydroxylysine residues.

Cited by 52 publications

References 23 publications

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

CP4: a pneumocyte-derived collagenous surfactant-associated protein. Evidence for heterogeneity of collagenous surfactant proteins

The repertoire of glycan determinants in the human glycome

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