Identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers Apostichopus japonicus (Selenka)

Liu, Hongzhan; Zheng, Fang; Sun, Xiaoqin; Hong, Xiao; Dong, Shuanglin; Wang, Bo; Tang, Xuexi; Wang, Yongqiang

doi:10.1016/j.jip.2010.05.016

Cited by 137 publications

(57 citation statements)

References 7 publications

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“…Unfortunately, the natural sources of A. japonicus in China have declined drastically, owing to outbreaks of various viral and bacterial diseases (27). Among these diseases, skin ulceration syndrome (SUS) causes high infectious and lethal rates and has received substantial attention (27, 28). In our previous work, we have found that miR-31 and miR-2008 expression levels are markedly increased in sea cucumbers with SUS by high-throughput analyses, thus indicating that these molecules play crucial roles in this host–pathogen interaction (29).…”

Section: Introductionmentioning

confidence: 99%

miR-31 Links Lipid Metabolism and Cell Apoptosis in Bacteria-Challenged Apostichopus japonicus via Targeting CTRP9

Shao

et al. 2017

Front. Immunol.

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The biological functions of microRNAs (miRNAs) have been studied in a number of eukaryotic species. Recent studies on vertebrate animals have demonstrated critical roles of miRNA in immune and metabolic activities. However, studies on the functions of miRNA in invertebrates are very limited. Here, we demonstrated that miR-31 from Apostichopus japonicus disrupts the balance of lipid metabolism, thus resulting in cell apoptosis by targeting complement C1q tumor necrosis factor-related protein 9 (AjCTRP9), a novel adipokine with pleiotropic functions in immunity and metabolism. Lipidomic analysis suggested that the intercellular lipid metabolites were markedly altered, and three ceramide (Cer) species synchronously increased in the AjCTRP9-silenced coelomocytes. Moreover, exogenous Cer exposure significantly induced apoptosis in the coelomocytes in vivo, in agreement with findings from miR-31 mimic- or AjCTRP9 small-interfering RNA-transfected coelomocytes. Furthermore, we found that the imbalance in sphingolipid metabolism triggered by the overproduction of Cers ultimately resulted in the activation of the apoptosis initiator caspase-8 and executioner caspase-3. Our findings provide the first direct evidence that miR-31 negatively modulates the expression of AjCTRP9 and disturbance of Cer channels, thus leading to caspase-3- and caspase-8-dependent apoptosis, during the interactions between pathogens and host.

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Section: Introductionmentioning

confidence: 99%

miR-31 Links Lipid Metabolism and Cell Apoptosis in Bacteria-Challenged Apostichopus japonicus via Targeting CTRP9

Shao

et al. 2017

Front. Immunol.

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“…It was roughly estimated that the species culture area in China exceeded one million acres, and a production value was over 150 million dollars in 2009 [1,2]. However, various diseases caused by bacteria and protozoa occurred in cultured A. japonicus populations with the development of intensive culture and environmental deterioration, resulting in enormous losses of the species aquaculture and being one of the limiting factors in the sustainable development of this industry [3].…”

Section: Introductionmentioning

confidence: 99%

Characterization of skin ulceration syndrome associated microRNAs in sea cucumber Apostichopus japonicus by deep sequencing

Feng

Qiu

et al. 2012

Fish & Shellfish Immunology

107

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“…As a dominant aquaculture species in china, its aquaculture industry has grown rapidly over recent years. The annual production of sea cucumber had reached 200,000 tons in China by 2014, with the culture area exceeding one million acres and a production value over 3,000,000,000 dollars [2,3]. Nevertheless, serious individual growth variation is often found in both nature and culture populations of sea cucumber.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the sea cucumber (Apostichopus japonicus) with variation in individual growth

Gao

Bao

et al. 2017

PLoS ONE

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The sea cucumber (Apostichopus japonicus) is an economically important aquaculture species in China. However, the serious individual growth variation often caused financial losses to farmers and the genetic mechanisms are poorly understood. In the present study, the extensively analysis at the transcriptome level for individual growth variation in sea cucumber was carried out. A total of 118946 unigenes were assembled from 255861 transcripts, with N50 of 1700. Of all unigenes, about 23% were identified with at least one significant match to known databases. In all four pair of comparison, 1840 genes were found to be expressed differently. Global hypometabolism was found to be occurred in the slow growing population, based on which the hypothesis was raised that growth retardation in individual growth variation of sea cucumber is one type of dormancy which is used to be against to adverse circumstances. Besides, the pathways such as ECM-receptor interaction and focal adhesion were enriched in the maintenance of cell and tissue structure and communication. Further, 76645 SSRs, 765242 SNPs and 146886 ins-dels were detected in the current study providing an extensive set of data for future studies of genetic mapping and selective breeding. In summary, these results will provides deep insight into the molecular basis of individual growth variation in marine invertebrates, and be valuable for understanding the physiological differences of growth process.

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Identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers Apostichopus japonicus (Selenka)

Cited by 137 publications

References 7 publications

miR-31 Links Lipid Metabolism and Cell Apoptosis in Bacteria-Challenged Apostichopus japonicus via Targeting CTRP9

miR-31 Links Lipid Metabolism and Cell Apoptosis in Bacteria-Challenged Apostichopus japonicus via Targeting CTRP9

Characterization of skin ulceration syndrome associated microRNAs in sea cucumber Apostichopus japonicus by deep sequencing

Transcriptome analysis of the sea cucumber (Apostichopus japonicus) with variation in individual growth

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