Identification of the Pancreatic Endocrine Cells of Pseudemys scripta elegans by Immunogold Labeling

Lozano, Marı́a Teresa; Hernández, M. P. García; Ayala, Alfonsa Garcı́a; Elbal, M.T.; Agulleiro, B.

doi:10.1006/gcen.2000.7394

Cited by 7 publications

(11 citation statements)

References 28 publications

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“…The same pattern of population of endocrine cells has also been reported in the endocrine pancreas of amphibia (Lee et al 2003). However, B-and A-cells are most common and the major pancreatic endocrine cell types found in Lissemys turtles as has been reported in other turtle species, Pseudemys scripta elegans (Lozano et al 2000) and lizard, Anolis (Rhoten & Smith 1978), and in all other vertebrates studied. In addition, there are several other types of endocrine cells in turtles and other vertebrates.…”

Section: Discussionsupporting

confidence: 78%

“…In addition, there are several other types of endocrine cells in turtles and other vertebrates. The pancreatic endocrine cell types are also highly variable in different chelonian species with two types (B-and non-B-cells) in three species of freshwater turtles, Chrysemys dorbigni, Phrynops hilarii (Cardeza 1957), Geoemyda pulcherrima (Miller 1962), five types (A-, B-, D-, PP-and Vth-type) in Testudo graeca (Garcia Ayala et al 1987) and A-, B-, D-, PP-and glucagon-NPY types in Pseudemys scripta elegans (Lozano et al 2000). Earlier reports indicate that poikilotherms, especially chelonians, have maximum number of pancreatic endocrine cell types (16 types in reptiles) compared to homeothermic vertebrates (six types) (Figure 11).…”

Section: Discussionmentioning

confidence: 99%

“…Functions of B-and A-cells are well known in chelonians (Lozano et al 2000). D-and PP-cells are known to produce somatostatin and pancreatic polypeptide, respectively, in turtles Testudo graeca (Garcia Ayala et al 1987) and Pseudemys scripta elegans (Agulleiro et al 1985;Lozano et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…The gastroenteropancreatic (GEP) endocrine system, studied by immunocytochemical technique, showed six types of immunoreactive endocrine cells -namely, insulin-(B), glucagon-(A), somatostatin-(D), pancreatic polypeptide-(PP), secretin, and peptide tyrosine tyrosine (PYY) -in the pancreas of two species of turtles, Testudo graeca (Family Testudinidae) and Mauremys caspica (Family Geoemydidae) (Perez-Tomas et al 1989). Lozano et al (2000), using the immunogold antibody method, have identified six types of immunoreactive cells -namely, insulin-(B), glucagon-(A), Salmon (S) somatostatin-(SST-14, SST-25 and SST-28), pancreatic polypeptide-(PP), peptide tyrosine tyrosine (PYY) and neuropeptide tyrosine (NPY) like immunoreactivities -in the endocrine pancreas of the turtle Pseudemys scripta elegans belonging to the Family Emydidae. The latter authors have identified cell types from ultrastructural characteristics of the secretory granules.…”

Section: Introductionmentioning

confidence: 99%

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Tinctorial, immunocytochemical and ultrastructural characterizations of the endocrine cell types of the pancreas of the soft-shelled turtle,Lissemys punctata punctata(Bonnoterre) (Reptilia: Chelonia)

Sarkar

Maiti

2011

Italian Journal of Zoology

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The objective of the present study was to identify the cell types of the endocrine pancreas by tinctorial, immunocytochemical and ultrastructural characteristics in Lissemys punctata punctata turtles. (a) B-cells were identified by fine light purple cytoplasmic granules tinctorially, insulin antibody reaction (brown colour), and mitochondrial zone with crystalline-like secretory granules ultrastructurally. (b) A-cells were characterized by very fine orangeophilic granules tinctorially, glucagon antibody reaction (bluish colour), and rough endoplasmic reticular (RER) and mitochondrial zones with larger secretory granules ultrastructurally. Other cell types were identified from ultrastructural characteristics. (c) D-cells had RER zone with clustered large secretory granules. (d) PP-cells showed fusiform shape with tapering ends, RER and mitochondrial zones, and small secretory granules. (e) Vth-cell type was characterized by triangular shape with mitochondrial zone and clustered secretory granules. (f) VIth-cell type was identified by pyriform shape, mitochondrial zone, and least population of clustered secretory granules. We conclude that the occurrence of several types of pancreatic endocrine cells may be related to the adaptation of Lissemys turtles.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tinctorial, immunocytochemical and ultrastructural characterizations of the endocrine cell types of the pancreas of the soft-shelled turtle,Lissemys punctata punctata(Bonnoterre) (Reptilia: Chelonia)

Sarkar

Maiti

2011

Italian Journal of Zoology

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“…Islet endocrine cells were also found IR for NPY in hamster (Myrsen et al, 1995a, b), in rat (Myrsen-Axcrona et al, 1997) and in man (Bennet et al, 1996). In non mammal vertebrates, NPY immunoreactivity was found in the pancreas of fish (Cheung et al, 1991) amphibians (Putti et al, 1995(Putti et al, , 1997 and reptiles (Putti et al, 1991;Delia Rossa & Putti, 1995;Lozano et al, 2000). In chicks, intracerebroventriculary administered NPY altered plasma insulin, glucagon and glucose concentrations: plasma insulin significantly increased (Kuenzel & McMurtry, 1988) demonstrating peripheral effects of this neuropeptide.…”

Section: Introductionmentioning

confidence: 99%

Effects of neuropeptide Y on food intake, glycemia and pancreatic secretion in the lizardPodarcis s. siculain early spring

Buono

Odierna

Putti³

2004

Italian Journal of Zoology

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In early spring, neuropeptide Y injections in the lizard Podarcis s. sicula caused an increase of appetite in animals compared with those injected with saline alone. Glycemia determination showed an annual cycle with a maximum of blood glucose in summer when animals feed, and a minimum in winter when they undergo a semi-hibernation period during which they do not eat. In NPYtreated lizards, blood glucose dropped as the NPY dose increased to reach significantly lower values than those of controls fed for the whole time of treatment. Both endocrine and exocrine pancreas was affected by the treatment. In particular, the insulin immunoreactive B cells appeared less intensely immunostained than those of the controls, with a progressive reduction of immunostaining, according to the NPY doses used. Optical density of immunostaining measurements of B cells, showing a progressive reduction of this parameter in the treated lizards, supported the morphological observations. Acinar cells, too, exhibited reduced immunostaining when tested for carboxypeptidase. Taken together, the findings suggest that exogenous NPY stimulated an increase of food intake, and basal insulin secretion, but lowered blood glucose by a direct and/or indirect mechanism via insulin secretion.

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Morphology of the pancreas of some species belonging to the genera Phelsuma and Gecko (family Gekkonidae): evidence of apoptotic process during the seasonal cycle

2006

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In this study we investigated comparative morphology of the endocrine pancreas of several species belonging to the family Gekkonidae and apoptotic processes of the pancreas which may be correlated to the seasonal cycle. The following species of the family Gekkonidae were studied: Phelsuma lineata, P. madagascariensis, P. dubia, P. abotti, Gekko gecko, G. vittatus, and Geckonia chazaliae. In all these species the pancreas consisted of large and medium islets as well as endocrine cells which were scattered throughout the acinar cells. Exocrine parenchyma consisted of tubuli-acini. Four mayor cell types were identified in the endocrine pancreas, using immunocytochemistry: glucagon-immunoreactive (A) cells, insulin-immunoreactive (B) cells, somatostatin-immunoreactive (D) cells, and pancreatic polypeptide immunoreactive (PP) cells. In the endocrine pancreas the amount of A cells and B cells was either equal or a prevalence of A cells was observed. In the wet season the pancreatic morphology presented normal features with very rare apoptotic cells. The animals belonging to the genus Phelsuma taken in the dry season (July) showed numerous vacuolated, Caspase 3, 9 and 11-immunoreactive acinar and some endocrine cells containing picnotic nuclei which were positive to tunel reaction. The animals belonging to the genus Gekko taken at the end of the dry season (October) exhibited strongly vacuolated, Caspase 3, 9 and 11-immunoreactive endocrine and some acinar cells containing nuclei which were positive to tunel reaction. These apoptosis events could be a reaction in response to stress mechanisms, such as a starvation period during the dry season.

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Identification of the Pancreatic Endocrine Cells of Pseudemys scripta elegans by Immunogold Labeling

Cited by 7 publications

References 28 publications

Tinctorial, immunocytochemical and ultrastructural characterizations of the endocrine cell types of the pancreas of the soft-shelled turtle,Lissemys punctata punctata(Bonnoterre) (Reptilia: Chelonia)

Tinctorial, immunocytochemical and ultrastructural characterizations of the endocrine cell types of the pancreas of the soft-shelled turtle,Lissemys punctata punctata(Bonnoterre) (Reptilia: Chelonia)

Effects of neuropeptide Y on food intake, glycemia and pancreatic secretion in the lizardPodarcis s. siculain early spring

Morphology of the pancreas of some species belonging to the genera Phelsuma and Gecko (family Gekkonidae): evidence of apoptotic process during the seasonal cycle

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