Identification of the novel lapine rotavirus genotype P[22] from an outbreak of enteritis in a Hungarian rabbitry

Bányai, Krisztiàn; Forgách, Petra; Erdélyi, Károly; Martella, Vito; Bogdan, A. T.; Hocsák, Enikö; Havasi, Viktória; Melegh, Béla; Szücs, György

doi:10.1016/j.virusres.2005.03.029

Cited by 21 publications

(32 citation statements)

References 34 publications

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“…During the investigation of local strain diversity in rabbit herds, two unusual rotavirus strains, LRV-4 and 3489/3, were identified by the analysis of their RNA profiles. Both strains displayed a super-short E-type of RNA migration by PAGE (Bányai et al, 2005;. LRV-4, a P[14],G3 rotavirus, was identified in an Italian rabbitry in Foggia, Italy, in 2003 .…”

Section: Acta Veterinaria Hungarica 57 2009mentioning

confidence: 99%

“…LRV-4, a P[14],G3 rotavirus, was identified in an Italian rabbitry in Foggia, Italy, in 2003 . Strain 3489/3 is a lapine P[22],G3 rotavirus, identified from the intestinal content of a dead rabbit in 2004 in Hungary (Bányai et al, 2005). The sequence of genome segment 11 of these two lapine strains was determined and analysed along with rearranged and normal cognate genome segments of other rotavirus strains of lapine origin.…”

Section: Acta Veterinaria Hungarica 57 2009mentioning

confidence: 99%

“…The nucleotide sequence of genome segment 11 of the two strains was determined by direct sequencing after amplification of the dsRNA by reverse transcription-PCR (Bányai et al, 2005;Martella et al, 2005). The alignment of genome segments 11 was prepared with the Multalin (Corpet, 1988) server and then manually adjusted using the GeneDoc software to obtain the best matches (Nicholas et al, 1997).…”

Section: Acta Veterinaria Hungarica 57 2009mentioning

confidence: 99%

“…Such super-short Etype has been observed rarely in bovine (VMRI), porcine (MC345, CC86, CC117, C60) simian (YK-1) and human (69M, B37, B38, AU19, Z10262) strains (Paul et al, 1988;González et al, 1989;Nuttall et al, 1989;Scott et al, 1989;Mattion et al, 1990;Ciarlet et al, 1997;Palombo et al, 1998;Nakagomi et al, 1999;Westerman et al, 2006). In addition, super-short RNA profile has been observed frequently in lapine rotaviruses (ALA, BAPwt, BAP-2, 3489/3, LRV-4; Thouless et al, 1986;Tanaka et al, 1988;Bányai et al, 2005;Martella et al, 2005) and in a lapine-like human strain, B4106, detected in Belgium (De Leener et al, 2004;Matthijnssens et al, 2006a). In addition, some tissue-culture adapted lapine rotavirus (LRV) strains has atypical long genome pattern (strain C-11) due to abnormal mobility of genome segment 10 or a short pattern (strain R-2) due to slower migration of genome segment 11 (Thouless et al, 1986).…”

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Frequent rearrangement may explain the structural heterogeneity in the 11th genome segment of lapine rotaviruses — Short communication

Bányai¹,

Matthijnssens

Szücs

et al. 2009

Acta Veterinaria Hungarica

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In rotaviruses, intragenic recombination or gene rearrangement occurs almost exclusively in the genome segments encoding for non-structural proteins. Rearranged RNA originates by mechanisms of partial sequence duplications and deletions or insertions of non-templated nucleotides. Of interest, epidemiological investigations have pointed out an unusual bias to rearrangements in genome segment 11, notably in rotavirus strains of lapine origin, as evidenced by the detection of numerous lapine strains with super-short genomic electropherotype. The sequence of the full-length genome segment 11 of two lapine strains with super-short electropherotype, LRV-4 and 3489/3, was determined and compared with rearranged and normal cognate genome segments of lapine rotaviruses. The rearranged genome segments contained head-to-tail partial duplications at the 3' end of the main ORF encoding NSP5. Unlike the strains Alabama and B4106, intermingled stretches of non-templated sequences were not present in the accessory RNA of LRV-4 and 3489/3, while multiple deletions were mapped, suggesting the lack of functional constraints. Altogether, these findings suggest that independent rearrangement events have given origin to the various lapine strains that have super-short genome pattern.

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Section: Acta Veterinaria Hungarica 57 2009mentioning

confidence: 99%

Section: Acta Veterinaria Hungarica 57 2009mentioning

confidence: 99%

Section: Acta Veterinaria Hungarica 57 2009mentioning

confidence: 99%

mentioning

confidence: 99%

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Frequent rearrangement may explain the structural heterogeneity in the 11th genome segment of lapine rotaviruses — Short communication

Bányai¹,

Matthijnssens

Szücs

et al. 2009

Acta Veterinaria Hungarica

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“…Já foram relatados pelo menos 16 diferentes tipos G e 27 genotipos P de rotavírus (Parra et al 2008) sendo que aparentemente há uma predominância de certos genotipos infectando determinadas espécies hospedeiras (Bányai 2005). Sua diversidade genética está associada a diferentes mecanismos de evolução os quais envolvem mutações pontuais (drifts); reestruturações (shifts, reassortants) ou troca de segmentos de RNA entre amostras; e rearranjos (rearrangements), que consistem em duplicações e deleções de sequência nucleotídica dentro de um segmento genômico (Taniguchi & Urasawa 1995).…”

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Diversidade genotípica de rotavírus suínos no Estado de São Paulo

et al. 2009

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Rotavírus é uma das causas mais comuns de diarréia tanto em humanos quanto em diferentes espécies animais. Foi conduzido um estudo transversal a partir de 144 amostras fecais diarréicas colhidas de leitões, provenientes de 16 criações comerciais distribuídas por 10 municípios do Estado de São Paulo, Brasil, com o objetivo de se detectar a ocorrência de rotavírus e realizar sua caracterização molecular quanto seus genotipos G e P. Um total de 43 amostras (29,86%) foram positivas para rotavírus por Eletroforese em Gel de Poliacrilamida (PAGE) e ELISA, num esquema de triagem em paralelo. A caracterização mediante reações do tipo nested-multiplex RT-PCR demonstrou que, isoladamente, o genotipo P[6] foi o mais frequente, detectado em 25,58% das amostras, seguido pelo P[1] (11,63%) e P[7] (9,3%). Infecções concomitantes de genotipos P[6]+P[7] (9,3%), P[1]+P[6] (4,65%), P[1]+P[6]+P[7] (2,33%) foram também observadas. Analogamente, o genotipo G[5] foi detectado em 30,23% das amostras, seguido pelo G[10] (20,93%) e G[6] (4,65%) e G[5]+G[10] (18,6%). O genotipo G[5]P[6] foi o mais frequente (11,63%), porém outras combinações e amostras não tipificáveis também foram observadas. Considerando-se a diversidade de rotavírus suínos encontrada na população estudada, medidas profiláticas específicas devem levar em conta, para sua efetividade, o grau de proteção cruzada entre os genotipos presentes nas formulações vacinais e aqueles que realmente são circulantes numa região.

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Pathology of Laboratory Rodents and Rabbits

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Identification of the novel lapine rotavirus genotype P[22] from an outbreak of enteritis in a Hungarian rabbitry

Cited by 21 publications

References 34 publications

Frequent rearrangement may explain the structural heterogeneity in the 11th genome segment of lapine rotaviruses — Short communication

Frequent rearrangement may explain the structural heterogeneity in the 11th genome segment of lapine rotaviruses — Short communication

Diversidade genotípica de rotavírus suínos no Estado de São Paulo

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