Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila

Ida, Takanori; Takahashi, Tomoko; Tominaga, Hatsumi; Sato, Takahiro; Kume, Kazuhiko; Ozaki, Mamiko; Hiraguchi, Tetsutaro; Maeda, Toru; Shiotani, Hajime; Terajima, Saki; Sano, Hiroko; Mori, Koichi; Yoshida, Morikatsu; Miyazato, Minoru; Kato, Jun; Murakami, Noboru; Kangawa, Kenji; Kojima, Masayasu

doi:10.1016/j.bbrc.2011.06.081

Cited by 53 publications

(61 citation statements)

References 19 publications

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“…Initial studies suggest a role in feeding behavior and/or digestion, a hypothesis strengthened by the expression pattern of its receptor, which mainly occurs in the hindgut [104,105]. The RYamide gene in G. m. morsitans contains at least two RYamide neuropeptides, both of which we were able to detect in the brain and TG.…”

Section: Ryamidementioning

confidence: 64%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Neuropeptides and peptide hormones are essential signaling molecules that regulate nearly all physiological processes. The recent release of the tsetse fly genome allowed the construction of a detailed in silico neuropeptide database (International Glossina Genome Consortium, Science 344, 380-386 (2014)), as well as an in-depth mass spectrometric analysis of the most important neuropeptidergic tissues of this medically and economically important insect species. Mass spectrometric confirmation of predicted peptides is a vital step in the functional characterization of neuropeptides, as in vivo peptides can be modified, cleaved, or even mispredicted. Using a nanoscale reversed phase liquid chromatography coupled to a Q Exactive Orbitrap mass spectrometer, we detected 51 putative bioactive neuropeptides encoded by 19 precursors: adipokinetic hormone (AKH) I and II, allatostatin A and B, capability/ pyrokinin (capa/PK), corazonin, calcitonin-like diuretic hormone (CT/DH), FMRFamide, hugin, leucokinin, myosuppressin, natalisin, neuropeptide-like precursor (NPLP) 1, orcokinin, pigment dispersing factor (PDF), RYamide, SIFamide, short neuropeptide F (sNPF) and tachykinin. In addition, propeptides, truncated and spacer peptides derived from seven additional precursors were found, and include the precursors of allatostatin C, crustacean cardioactive peptide, corticotropin releasing factor-like diuretic hormone (CRF/DH), ecdysis triggering hormone (ETH), ion transport peptide (ITP), neuropeptide F, and proctolin, respectively. The majority of the identified neuropeptides are present in the central nervous system, with only a limited number of peptides in the corpora cardiaca-corpora allata and midgut. Owing to the large number of identified peptides, this study can be used as a reference for comparative studies in other insects.

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Section: Ryamidementioning

confidence: 64%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

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“…Similar to the effect of injection of HP-I in A. aegypti, where host-seeking was inhibited, injection of this dRYamide-1 in the black blow fly (Phormia regina Meigen, 1826) resulted in a reduced sensitivity to sugar in the medium, suggesting that dRYamides attenuated the feeding motivation of the flies (Ida et al 2011). …”

Section: Neuropeptide F and Short Neuropeptide Fmentioning

confidence: 77%

Peptidergic control of food intake and digestion in insects¹This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.

et al. 2012

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Like all heterotrophic organisms, insects require a strict control of food intake and efficient digestion of food into nutrients to maintain homeostasis and to fulfill physiological tasks. Feeding and digestion are steered by both external and internal signals that are transduced by a multitude of regulatory factors, delivered either by neurons innervating the gut or mouthparts, or by midgut endocrine cells. The present review gives an overview of peptide regulators known to control feeding and digestion in insects. We describe the discovery and functional role in these processes for insect allatoregulatory peptides, diuretic hormones, FMRFamide-related peptides, (short) neuropeptide F, proctolin, saliva production stimulating peptides, kinins, and tachykinins. These peptides control either gut myoactivity, food intake, and (or) release of digestive enzymes. Some peptides exert their action at multiple levels, possibly having a biological function that depends on their site of delivery. Many regulatory peptides have been physically extracted from different insect species. However, multiple peptidomics, proteomics, transcriptomics, and genome sequencing projects have led to increased discovery and prediction of peptide (precursor) and receptor sequences. In combination with physiological experiments, these large-scale projects have already led to important steps forward in unraveling the physiology of feeding and digestion in insects.Key words: insect, midgut, digestion, neuropeptides, stomatogastric nervous system. Résumé : Comme tous les organismes hétérotrophes, les insectes requièrent un contrôle strict de leur ingestion de nourriture et la digestion de leur nourriture en nutriments afin de maintenir leur homéostasie et de compléter leurs tâches physiologiques. L'alimentation et la digestion sont gouvernées par des signaux tant externes qu'internes qui sont transmis par un multitude de facteurs régulateurs, livrés ou bien par les neurones qui innervent le tube digestif ou les pièces buccales ou alors par les cellules endocrines du tube digestif moyen. Notre revue fournit une synthèse des régulateurs peptidiques connus pour contrôler l'alimentation et la digestion chez les insectes. Nous décrivons la découverte et le rôle fonctionnel de ces processus dans le cas des peptides allatorégulateurs, des hormones diurétiques, des peptides apparentés aux amides FMRF, du neuropeptide F (court), de la proctoline, des peptides stimulateurs de la production de salive, des kinines et des tachykinines des insectes. Ces peptides contrôlent la myoactivité du tube digestif, l'ingestion de nourriture et(ou) la libération des enzymes digestives. Certains peptides agissent à de multiples niveaux, possédant une fonction biologique qui dépend de leur site d'action. Plusieurs peptides régulateurs ont été extraits physiquement de différentes espèces d'insectes. Cependant, plusieurs projets de peptidomique, de protéomique, de transcriptomique et de génomique ont mené à de nombreuses découvertes supplémentaires et des prédict...

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“…RNAseq data suggests that both these genes are approximately equally expressed. The Drosophila and Tribolium RYamide GPCRs were recently identified (Ida et al, 2011a;Collin et al, 2011) and a Tetranychus ortholog (tetur08g06060) is present in the genome assembly which is thus probably the spider mite receptor as it is both very similar to its insect orthologs (best BLAST e-value of 2e-110) and close to them on the phylogenetic tree (Fig. 13).…”

Section: Ryamidementioning

confidence: 95%

In silico cloning of genes encoding neuropeptides, neurohormones and their putative G-protein coupled receptors in a spider mite

Veenstra

Rombauts

Grbić

2012

Insect Biochemistry and Molecular Biology

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Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila

Cited by 53 publications

References 19 publications

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

In silico cloning of genes encoding neuropeptides, neurohormones and their putative G-protein coupled receptors in a spider mite

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