1991
DOI: 10.1016/0006-291x(91)91616-k
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Identification of the N-terminal residue of the heavy chain of both native and recombinant human hepatocyte growth factor

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Cited by 56 publications
(18 citation statements)
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“…Recombinant human HGF (rhHGF) was prepared as described previously. 26 rhHGF was dissolved in 10 mmol/L phosphate buffer (pH 7.4), containing 0.7 mol/L NaCl and 0.05% bovine serum albumin (Sigma Chemical Co., St. Louis, MO), with the final concentration adjusted to 1 mg/mL. In this model, 70% hepatectomy was found to be lethal when performed within 5 days after the last DMN injection in our preliminary study.…”
Section: Animalsmentioning
confidence: 99%
“…Recombinant human HGF (rhHGF) was prepared as described previously. 26 rhHGF was dissolved in 10 mmol/L phosphate buffer (pH 7.4), containing 0.7 mol/L NaCl and 0.05% bovine serum albumin (Sigma Chemical Co., St. Louis, MO), with the final concentration adjusted to 1 mg/mL. In this model, 70% hepatectomy was found to be lethal when performed within 5 days after the last DMN injection in our preliminary study.…”
Section: Animalsmentioning
confidence: 99%
“…Recombinant human HGF was prepared and purified as described previously (33). Other materials used in this study were described previously (2,22).…”
Section: Methodsmentioning
confidence: 99%
“…Recently, cytotoxic and cytostatic activities of SF/HGF have been reported (15)(16)(17). Mature SF/HGF of human fibroblasts is a 92-kDa disulfide-linked heterodimer that is translated as a single-chain precursor and then proteolyzed at a dibasic site; the heavy chain (62 kDa) consists of an N-terminal region that could form a hairpin structure followed by four kringle modules; the light chain (32/34 kDa) is homologous to serine proteases but has no demonstrable protease activity due to the absence of two of three critical residues within the catalytic site (2,4,6,7,(18)(19)(20). In human fibroblasts two major transcripts of SF/HGF [6 and 3 kilobases (kb)] exist due to differential poly(A) site usage (4).…”
mentioning
confidence: 99%