Identification of the more complex triacylglycerols in bovine milk fat by gas chromatography—mass spectrometry using polar capillary columns

Regiospecific analysis of TAGs using chromatography, MS, and chromatography‐MS

“…The 34.7 and that of L/S/S-S/L/S at 1.0 mol%, including a small proportion of S/S/S. In contrast to previous reports [43][44][45], the present data indicate, owing to the detection of S/L/S, the presence of a small proportion of butyric and caproic acids in the sn-1-position. The overall distribution of the fatty acids in the short-chain TAGs of butterfat, calculated from direct mass spectrometric measurements, was sn-1 (0.1), sn-2 (0.7), and sn-3 (99.2%) positions, is consistent with the results of indirect determinations based on stereospecific analyses of total butterfat.…”

Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS

“…The 12 SD rats (220-250 g body weight) were divided into two equivalent groups, one as the control group and the other as the tography (GC) and gas-chromatography mass spectrometry (GC-MS) were utilized (13)(14)(15)(16), prior to which time consuming hydrolysis and derivatization reactions of lipids should have been performed. This problem was solved with the advent of electrospray ionization (ESI), resulting in the indispensability of MS today in lipid research.…”

Purification and Structural Determination of Hematopoietic Stem Cell-Stimulating Monoacetyldiglycerides from Cervus nippon (Deer Antler)

“…16,17) The structures of several monoacetyldiglycerides have been confirmed by gas chromatography (GC), GC-mass spectroscopy (MS), high-performance liquid chromatography (HPLC), liquid chromatography (LC)-MS, nuclear magnetic resonance (NMR), infrared (IR), and single-crystal X-ray analysis. [16][17][18][19] Monoacetyldiglycerides were previously synthesized from corresponding phosphatidylethanolamines, phosphatidylcholines, phosphatidylglycerols, and diacylglycerols by acetolysis and enzymolysis followed by acetylation or esterification.…”

“…16,17) The structures of several monoacetyldiglycerides have been confirmed by gas chromatography (GC), GC-mass spectroscopy (MS), high-performance liquid chromatography (HPLC), liquid chromatography (LC)-MS, nuclear magnetic resonance (NMR), infrared (IR), and single-crystal X-ray analysis. [16][17][18][19] Monoacetyldiglycerides were previously synthesized from corresponding phosphatidylethanolamines, phosphatidylcholines, phosphatidylglycerols, and diacylglycerols by acetolysis and enzymolysis followed by acetylation or esterification. [20][21][22] The water extract of deer antler exhibited interesting growth-promotion effects on the hematopoietic system in our preliminary in vitro experiment, and hence several fractionation and purification steps were utilized to search for the underlying active components.…”