Identification of the minimal replication region of the multicopy Streptomyces plasmid pSL1

Shindoh, Yutaka; Urabe, Hiroaki; Nakano, Michiko; Ogawara, Hiroshi

doi:10.1016/0147-619x(87)90020-5

Cited by 8 publications

(1 citation statement)

References 23 publications

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“…The mmr gene shows structural similarities to tetracycline resistance genes of eubacteria (105) (115), primarily for transfer between Escherichia coli and streptomycetes. For several plasmids, those functions involved in pock formation (1), plasmid transfer (76), minimal replication region (102,129), and incompatibility (143) have been localized. For plasmid pSL1 (129,143), the replication origin is an A+T-rich region flanked by two G+C-rich hairpin structures and resides near the incompatibility region, similar to the case with several E. coli plasmids.…”

Section: Plasmid-mediated Gene Cloningmentioning

confidence: 99%

Streptomyces cloning: useful recombinant DNA systems and a summation of cloned genes

Tomich

1988

Antimicrob Agents Chemother

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Section: Plasmid-mediated Gene Cloningmentioning

confidence: 99%

Streptomyces cloning: useful recombinant DNA systems and a summation of cloned genes

Tomich

1988

Antimicrob Agents Chemother

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The Streptomyces aureofaciens plasmid pIMB R8 and its use for shuttle vector construction

Godány

Lacová

Zelinka

1990

J. Basic Microbiol.

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The cryptic low copy plasmid designated as pIMB R8 was isolated from an industrial strain of the chlortetracycline producer Streptomyces aureofaciens R8/26. Using restriction endonucleases the pIMB R8 plasmid was characterized to have 15 kb. Subcloning of the Bam HI fragments of pIMB R8 into replication probe vector resulted in the identification of the replication part. The 0.7 kb Bc/I--Bg/I fragment is sufficient for normal replication, but produces about fourty times high plasmid copy numbers than the original pIMB R8. Using the replication part of pIMB R8 was constructed several shuttle cloning vectors for the E. coli-streptomycete system.

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Replication of the Streptomyces plasmid pSN22 through single-stranded intermediates

et al. 1994

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The replication of the 11 kb conjugative multicopy Streptomyces plasmid pSN22 was analyzed. Mutation and complementation analyses indicated that the minimal region essential for plasmid replication was located on a 1.9 kb fragment of pSN22, containing a transacting element encoding a replication protein and a cis-acting sequence acting as a replication origin. Southern hybridization showed that minimal replicon plasmids accumulated much more single-stranded plasmid molecules than did wild-type pSN22. Only one strand was accumulated. A 500 bp fragment from the pSN22 transfer region was identified which reduced the relative amount of single-stranded DNA, when added in the native orientation to minimal replicon plasmids. This 500 bp DNA sequence may be an origin for second-strand synthesis. It had no effect on the efficiency of co-transformation, plasmid incompatibility, or stability. The results indicate that pSN22 replicates via single-stranded intermediates by a rolling circle mechanism.

show abstract

Identification of the minimal replication region of the multicopy Streptomyces plasmid pSL1

Cited by 8 publications

References 23 publications

Streptomyces cloning: useful recombinant DNA systems and a summation of cloned genes

Streptomyces cloning: useful recombinant DNA systems and a summation of cloned genes

The Streptomyces aureofaciens plasmid pIMB R8 and its use for shuttle vector construction

Replication of the Streptomyces plasmid pSN22 through single-stranded intermediates

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