Identification of the metastasis potential and its associated genes in melanoma multinucleated giant cells using the PHA-ECM830 fusion method

Mi, Ruifang; Pan, Chunxiao; Zhou, Yiqiang; Liu, Yuanbo; Jin, Guishan; Liu, Fusheng

doi:10.3892/or.2015.4376

Cited by 15 publications

(11 citation statements)

References 27 publications

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“…2C , right panel). In addition, we found commonly regulated genes associated with metastasis; TUBB2B 28 and PMEPA1 29 , besides NGFR. Further exploration of genes differentially regulated among the CD271 high and CD271 low BM subsets revealed commonly up-regulated (n = 10) and down-regulated (n = 5) genes.…”

Section: Resultsmentioning

confidence: 75%

CD271 determines migratory properties of melanoma cells

Radke

Roßner

Redmer

2017

Sci Rep

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Melanoma cell expression of the nerve growth factor receptor CD271 is associated with stem-like properties. However, the contributing role of the receptor in melanoma cell migration is elusive. Here, we explored extracranial (skin, soft tissue, lymph node and liver, n = 13) and matched brain metastases (BM, n = 12) and observed a heterogeneous distribution of phenotypically distinct subsets of CD271+ cells. In addition, we observed that CD271 expression gradually rises along with melanoma progression and metastasis by exploration of publicly available expression data of nevi, primary melanoma (n = 31) and melanoma metastases (n = 54). Furthermore, we observed highest levels of CD271 in BM. Sub-clustering identified 99 genes differentially expressed among CD271high and CD271low (p < 0.05) BM-subgroups. Comparative analysis of subsets revealed increased ( ≥ 1.5fold, log2) expression of migration-associated genes and enrichment of CD271-responsible genes involved in DNA-repair and stemness. Live cell-imaging based scratch-wound assays of melanoma cells with stable knock-down of CD271 revealed a significantly reduced cell migration (3.9fold, p = 1.2E-04) and a reduced expression of FGF13, CSPG4, HMGA2 and AKT3 major candidate regulatory genes of melanoma cell migration. In summary, we provide new insights in melanoma cell migration and suggest that CD271 serves as a candidate regulator, sufficient to determine cellular properties of melanoma brain metastatic cells.

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Section: Resultsmentioning

confidence: 75%

CD271 determines migratory properties of melanoma cells

Radke

Roßner

Redmer

2017

Sci Rep

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“…In melanoma, βIIb-tubulin mRNA transcript levels were significantly elevated in melanoma multinucleated giant cells which are frequently observed in metastatic sites and correlate with poor prognosis, indicating that this tubulin isotype may play an important role in melanoma metastasis [ 217 ]. Increased expression of βIVb-tubulin was observed in sentinel lymph node micrometastasis of colorectal cancer [ 218 ].…”

Section: Tubulin and Metastasismentioning

confidence: 99%

An Emerging Role for Tubulin Isotypes in Modulating Cancer Biology and Chemotherapy Resistance

Parker

Teo

McCarroll

et al. 2017

IJMS

112

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Tubulin proteins, as components of the microtubule cytoskeleton perform critical cellular functions throughout all phases of the cell cycle. Altered tubulin isotype composition of microtubules is emerging as a feature of aggressive and treatment refractory cancers. Emerging evidence highlighting a role for tubulin isotypes in differentially influencing microtubule behaviour and broader functional networks within cells is illuminating a complex role for tubulin isotypes regulating cancer biology and chemotherapy resistance. This review focuses on the role of different tubulin isotypes in microtubule dynamics as well as in oncogenic changes that provide a survival or proliferative advantage to cancer cells within the tumour microenvironment and during metastatic processes. Consideration of the role of tubulin isotypes beyond their structural function will be essential to improving the current clinical use of tubulin-targeted chemotherapy agents and informing the development of more effective cancer therapies.

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“…In the present study, multinucleated giant cells appeared in neoplastic lesions of xmrk -transgenic medaka of both the Carbio and HB11A strains. A previous study demonstrated that melanoma multinucleated giant cells may play an important role in melanoma metastasis, because they increased pulmonary metastasis ability and the β-tubulin gene group was upregulated in them 26 . Other previous studies showed that distant metastases occurred in the malignant melanomas with multinucleated giant cells of human and animals 1 , 3 , 27 , 28 , 29 .…”

mentioning

confidence: 99%

Histopathologic features of melanocytic tumors in Xiphophorus melanoma receptor kinase (xmrk)-transgenic medaka (Oryzias latipes)

2019

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Melanocytic tumors in Xiphophorus melanoma receptor kinase ( xmrk )-transgenic Carbio and HB11A strains of medaka were examined histopathologically at 7 months post-hatching. Medaka of both strains developed melanocytic tumors with a penetrance of 100%. In both strains, neoplastic cells containing intracytoplasmic melanin pigment granules showed significant invasive growth patterns. In addition, epithelioid neoplastic cells were arranged in solid nests, and spindle neoplastic cells were arranged in interlacing streams and bundles. Nuclear atypia, anisokaryosis, cellular pleomorphism, and the appearance of anaplastic giant cells containing multiple nuclei or a single nucleus were observed in neoplastic lesions in both medaka strains. However, neither strain exhibited mitotic figures or invasion of blood vessels by neoplastic cells. Based on these histopathologic findings, the tumors were diagnosed as malignant melanoma. This is the first report of detailed histomorphologic characteristics of malignant melanoma in xmrk -transgenic medaka.

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Identification of the metastasis potential and its associated genes in melanoma multinucleated giant cells using the PHA-ECM830 fusion method

Cited by 15 publications

References 27 publications

CD271 determines migratory properties of melanoma cells

CD271 determines migratory properties of melanoma cells

An Emerging Role for Tubulin Isotypes in Modulating Cancer Biology and Chemotherapy Resistance

Histopathologic features of melanocytic tumors in <i>Xiphophorus</i> melanoma receptor kinase (<i>xmrk</i>)-transgenic medaka (<i>Oryzias latipes</i>)

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