Identification of the MBF1 heat‐response regulon of <i>Arabidopsis thaliana</i>

Suzuki, Nobuhiro; Sejima, Hiroe; Tam, Rachel; Schlauch, Karen; Mittler, Ron

doi:10.1111/j.1365-313x.2011.04550.x

Cited by 144 publications

(130 citation statements)

References 28 publications

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“…In contrast, MBF1c was not required for the expression of transcripts encoding HSFA2 and various HSPs. However, Suzuki et al (2011) suggested that MBF1c in Arabidopsis functions as a transcriptional regulator that binds DNA and controls the expression of 36 different transcripts during heat stress, including the important transcriptional regulator DRE-BINDING PROTEIN2A, two HSFs (HSFB2B and HSFB2A), and several zinc finger proteins. Moreover, the DNAbinding domain of MBF1c had a dominant-negative effect on heat tolerance when constitutively expressed in plants.…”

Section: Some Important Transcript Factors Could Be Involved In Heat mentioning

confidence: 99%

Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

et al. 2017

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Heat stress is one of the primary abiotic stresses that limit crop production. Grape (Vitis vinifera) is a cultivated fruit with high economic value throughout the world, with its growth and development often influenced by high temperature. Alternative splicing (AS) is a widespread phenomenon increasing transcriptome and proteome diversity. We conducted high-temperature treatments (35°C, 40°C, and 45°C) on grapevines and assessed transcriptomic (especially AS) and proteomic changes in leaves. We found that nearly 70% of the genes were alternatively spliced under high temperature. Intron retention (IR), exon skipping, and alternative donor/acceptor sites were markedly induced under different high temperatures. Among all differential AS events, IR was the most abundant up-and down-regulated event. Moreover, the occurrence frequency of IR events at 40°C and 45°C was far higher than at 35°C. These results indicated that AS, especially IR, is an important posttranscriptional regulatory event during grape leaf responses to high temperature. Proteomic analysis showed that protein levels of the RNA-binding proteins SR45, SR30, and SR34 and the nuclear ribonucleic protein U1A gradually rose as ambient temperature increased, which revealed a reason why AS events occurred more frequently under high temperature. After integrating transcriptomic and proteomic data, we found that heat shock proteins and some important transcription factors such as MULTIPROTEIN BRIDGING FACTOR1c and HEAT SHOCK TRANSCRIPTION FACTOR A2 were involved mainly in heat tolerance in grape through up-regulating transcriptional (especially modulated by AS) and translational levels. To our knowledge, these results provide the first evidence for grape leaf responses to high temperature at simultaneous transcriptional, posttranscriptional, and translational levels.Heat stress is one of the main abiotic stresses limiting crop production worldwide. Global warming is predicted to be accompanied by more frequent and powerful extreme temperature events (Lobell et al., 2008). Therefore, a better understanding of the response of plants to heat stress is essential for improving their heat tolerance. In general, heat stress is a complex function of intensity (temperature in degrees), duration, and rate of increase in temperature. At very high temperatures, a catastrophic collapse of cellular organization may occur within minutes, which results in severe cellular injury and even cell death (Wahid et al., 2007). At moderately high temperatures, direct injuries include protein denaturation and aggregation and the increased fluidity of membrane lipids. Indirect or slower heat injuries include inactivation of enzymes, inhibition of protein synthesis, protein degradation, and loss of membrane integrity. These injuries eventually lead to a decline of net photosynthetic rate, reduced ion flux, production of toxic compounds and reactive oxygen species, and inhibition of growth. Actually, plants are not passively damaged by heat stress but respond to temperature changes by r...

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Section: Some Important Transcript Factors Could Be Involved In Heat mentioning

confidence: 99%

Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

et al. 2017

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“…performed grafting experiments between wild-type and rbohD seedlings and followed, as a marker for HS-induced SAA, the expression of the key HS response transcriptional regulator multiprotein-bridging factor1c (MBF1c; Suzuki et al, 2008Suzuki et al, , 2011a in systemic tissues in response to HS applied to local tissues. As shown in Figure 2, local application of HS to the cotyledons (local tissues) of control grafted seedlings resulted in the enhanced expression of MBF1c in root tips (systemic tissues).…”

Section: Biological Function and Regulation Of The Rapid Autopropagatmentioning

confidence: 99%

Temporal-Spatial Interaction between Reactive Oxygen Species and Abscisic Acid Regulates Rapid Systemic Acclimation in Plants

et al. 2013

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Being sessile organisms, plants evolved sophisticated acclimation mechanisms to cope with abiotic challenges in their environment. These are activated at the initial site of exposure to stress, as well as in systemic tissues that have not been subjected to stress (termed systemic acquired acclimation [SAA]). Although SAA is thought to play a key role in plant survival during stress, little is known about the signaling mechanisms underlying it. Here, we report that SAA in plants requires at least two different signals: an autopropagating wave of reactive oxygen species (ROS) that rapidly spreads from the initial site of exposure to the entire plant and a stress-specific signal that conveys abiotic stress specificity. We further demonstrate that SAA is stress specific and that a temporal-spatial interaction between ROS and abscisic acid regulates rapid SAA to heat stress in plants. In addition, we demonstrate that the rapid ROS signal is associated with the propagation of electric signals in Arabidopsis thaliana. Our findings unravel some of the basic signaling mechanisms underlying SAA in plants and reveal that signaling events and transcriptome and metabolome reprogramming of systemic tissues in response to abiotic stress occur at a much faster rate than previously envisioned.

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“…These HsfA1-downstream TFs include both enhancers and repressors of the HSR. HsfAs, DREB2A, and MBF1c have been reported to be positive regulators of the HSR (Sakuma et al, 2006;Charng et al, 2007;Schramm et al, 2008;Yoshida et al, 2008;Larkindale and Vierling, 2008;Suzuki et al, 2011;Nishizawa-Yokoi et al, 2011). These TFs enhance and sustain the expression of HS-inducible genes.…”

Section: Introductionmentioning

confidence: 99%

The Transcriptional Cascade in the Heat Stress Response of Arabidopsis Is Strictly Regulated at the Level of Transcription Factor Expression

et al. 2015

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Group A1 heat shock transcription factors (HsfA1s) are the master regulators of the heat stress response (HSR) in plants. Upon heat shock, HsfA1s trigger a transcriptional cascade that is composed of many transcription factors. Despite the importance of HsfA1s and their downstream transcriptional cascade in the acquisition of thermotolerance in plants, the molecular basis of their activation remains poorly understood. Here, domain analysis of HsfA1d, one of several HsfA1s in Arabidopsis thaliana, demonstrated that the central region of HsfA1d is a key regulatory domain that represses HsfA1d transactivation activity through interaction with HEAT SHOCK PROTEIN70 (HSP70) and HSP90. We designated this region as the temperature-dependent repression (TDR) domain. We found that HSP70 dissociates from HsfA1d in response to heat shock and that the dissociation is likely regulated by an as yet unknown activation mechanism, such as HsfA1d phosphorylation. Overexpression of constitutively active HsfA1d that lacked the TDR domain induced expression of heat shock proteins in the absence of heat stress, thereby conferring potent thermotolerance on the overexpressors. However, transcriptome analysis of the overexpressors demonstrated that the constitutively active HsfA1d could not trigger the complete transcriptional cascade under normal conditions, thereby indicating that other factors are necessary to fully induce the HSR. These complex regulatory mechanisms related to the transcriptional cascade may enable plants to respond resiliently to various heat stress conditions.

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Identification of the MBF1 heat‐response regulon of Arabidopsis thaliana

Cited by 144 publications

References 28 publications

Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

Temporal-Spatial Interaction between Reactive Oxygen Species and Abscisic Acid Regulates Rapid Systemic Acclimation in Plants

The Transcriptional Cascade in the Heat Stress Response of Arabidopsis Is Strictly Regulated at the Level of Transcription Factor Expression

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