Identification of the Key Molecular Drivers of Phosphorus Utilization Based on Host miRNA-mRNA and Gut Microbiome Interactions

Abstract

Phosphorus is an essential mineral for all living organisms and a limited resource worldwide. Variation and heritability of phosphorus utilization (PU) traits were observed, indicating the general possibility of improvement. Molecular mechanisms of PU, including host and microbial effects, are still poorly understood. The most promising molecules that interact between the microbiome and host are microRNAs. Japanese quail representing extremes for PU were selected from an F2 population for miRNA profiling of the ileal tissue and subsequent association with mRNA and microbial data of the same animals. Sixty-nine differentially expressed miRNAs were found, including 21 novel and 48 known miRNAs. Combining miRNAs and mRNAs based on correlated expression and target prediction revealed enrichment of transcripts in functional pathways involved in phosphate or bone metabolism such as RAN, estrogen receptor and Wnt signaling, and immune pathways. Out of 55 genera of microbiota, seven were found to be differentially abundant between PU groups. The study reveals molecular interactions occurring in the gut of quail which represent extremes for PU including miRNA-16-5p, miR-142b-5p, miR-148a-3p, CTDSP1, SMAD3, IGSF10, Bacteroides, and Alistipes as key indicators due to their trait-dependent differential expression and occurrence as hub-members of the network of molecular drivers of PU.

Mapping genes for phosphorus utilization and correlated traits using a 4k SNP linkage map in Japanese quail (Coturnix japonica)

“…2020) as well as miRNA–mRNA and gut microbiota (Ponsuksili et al . 2020) on the focal trait PU and other related traits. Preliminary QTL mapping was done on few chromosomes and markers, without reporting any clear signals (Beck et al .…”

A Multi-Omic Analysis for Low Bone Mineral Density in Postmenopausal Women Suggests a Relationship between Diet, Metabolites, and Microbiota

“…Remarkably, these amino acids correlated positively with the abundance of Bacteroides . Recently, Ponsuksili et al correlated Bacteroides abundance with several miRNAs and transcripts involved with bone metabolism and inflammatory response [ 43 ]. Bacteroides fragilis evoke an anti-inflammatory response by inducing Treg cells.…”

Clinical characteristics associated with the properties of gut microbiota in peritoneal dialysis patients

“…Alistipes participate in intestinal phosphorus absorption in animals, and so, similar mechanisms may exist in human being. 28 The effects of genera Lachnoclostridium and Anaerostipes on phosphorus metabolisms in CKD patients are not fully understood.…”