Identification of the key differentially expressed genes and pathways involved in neutrophilia

Abstract

Polymorphonuclear neutrophils (PMNs) are the most important determinants in the acute inflammatory response. Pathologically increased numbers of PMNs in the circulation or specific tissues (or both) lead to neutrophilia. However, the genes expressed and pathways involved in neutrophilia have yet to be elucidated. By analysis of three public microarray datasets related to neutrophilia (GSE64457, GSE54644, and GSE94923) and evaluation by gene ontology, pathway enrichment, protein–protein interaction networks, and hub genes analysis using multiple methods (DAVID, PATHER, Reactome, STRING, Reactome FI Plugin, and CytoHubba in Cytoscape), we identified the commonly up-regulated and down-regulated different expressed genes. We also discovered that multiple signaling pathways (IL-mediated, LPS-mediated, TNF-α, TLR cascades, MAPK, and PI3K-Akt) were involved in PMN regulation. Our findings suggest that the commonly expressed genes involved in regulation of multiple pathways were the underlying molecular mechanisms in the development of inflammatory, autoimmune, and hematologic diseases that share the common phenotypic characteristics of increased numbers of PMNs. Taken together, these data suggest that these genes are involved in the regulation of neutrophilia and that the corresponding gene products could serve as potential biomarkers and/or therapeutic targets for neutrophilia.

Enhancement of vitamin B6 production driven by omics analysis combined with fermentation optimization

“…RNA-seq is mainly applied to study transcriptomic differences caused by various treatments [ 22 ]. The overall analysis of transcription, translation and metabolism at the molecular level allowed the identification of key differentially expressed genes (DEGs) and provided new insights into the molecular mechanisms involved in target molecule production [ 23 ]. Zhu et al [ 24 ] used RNA-Seq to explore the effects of nitrogen sources and ICDH (isocitrate dehydrogenase) knockout on glycolate production in E. coli .…”

Transcriptome profile of spleen tissues from locally-adapted Kenyan pigs (Sus scrofa) experimentally infected with three varying doses of a highly virulent African swine fever virus genotype IX isolate: Ken12/busia.1 (ken-1033)

“…ASFV infection causes neutrophilia in pigs: a higher neutrophil count in the blood than the normal reference range typical during infections and inflammation [ 121 ]. We detected upregulated expression of colony-stimulating factor 3 receptor ( CSF3 ), also called the receptor for granulocyte colony-stimulating factor ( G-CSF ), that contributes to the proliferation and granulocyte differentiation of myeloid progenitor cells [ 122 ].…”

Integration of Airway Inflammation and Remodeling Mechanisms Specific to Eosinophilic Asthma Through Differential Co-Expression of Genes in Bronchial Brush Biopsy Samples

“…DGLUCY can be linked to asthma through (1) impact on E-cadherin expression (involved in to epithelial integrity in asthma), possibly through inhibition of ERK and P90RSK phosphorylation 134 , (2) its suppression by miRNA-199b (overexpressed during bacterial but not viral infections) 135 , (3) possibly related differential expression in neutrophilia 136 , (4) strong differential expression implied in seasonal remodeling of the immune system (reinforcing its role in immune system function and possibly regulation) 137 .…”