Identification of the initiation codon for the atpB gene in Chlamydomonas chloroplasts excludes translation of a precursor form of the β subunit of the ATP synthase

Rimbault, Blandine; Esposito, Donna; Drapier, Dominique; Choquet, Yves; Stern, David B.; Wollman, Françis Andrè

doi:10.1007/s004380000332

Cited by 18 publications

(19 citation statements)

References 24 publications

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“…Strains and Growth Conditions-We used the following C. reinhardtii wild-type strain T222ϩ, the double mutant pfl1-1 adh1 (33), which lacks the pyruvate formate-lyase and the alcohol dehydrogenase involved in fermentation pathways; the ATP synthase mutant Fud50.ORϩ (34), the ⌬rbcL 1-7.5 mutant, which lacks the large subunit of Rubisco (35); the plastocyanin mutant ac208 (36), the so-called pewy mutant, which accumulates the b 6 f complex but is impaired in its quinol oxidation site (37); the ⌬petA mutant, which lacks b 6 f complex (38); the dum22 mutant lacks both the mitochondrial complex I and bc 1 complexes (39), and the HydEF (40) and HydG (41) mutants that lack assembly factors required for hydrogenase maturation. Cells were grown to mid-log phase (3-5 10 6 cells per ml) in Tris/acetate/phosphate medium (42) at 25°C at 50 mol photons m Ϫ2 s Ϫ1 .…”

Section: Methodsmentioning

confidence: 99%

The Involvement of Hydrogen-producing and ATP-dependent NADPH-consuming Pathways in Setting the Redox Poise in the Chloroplast of Chlamydomonas reinhardtii in Anoxia

Clowez

Godaux

Cardol

et al. 2015

Journal of Biological Chemistry

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Background: Shift to anoxia promotes the over-reduction of photosystem I electron acceptors. Results: This over-reduction can be relieved by the activation of several pathways. Conclusion: The two mains pathways are the ATP-dependent CO 2 fixation pathway and the ATP-independent hydrogenase. Significance: We disentangle the role of the various NADPH-consuming pathways in setting the redox poise in the chloroplast of unicellular photosynthetic algae.

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Section: Methodsmentioning

confidence: 99%

The Involvement of Hydrogen-producing and ATP-dependent NADPH-consuming Pathways in Setting the Redox Poise in the Chloroplast of Chlamydomonas reinhardtii in Anoxia

Clowez

Godaux

Cardol

et al. 2015

Journal of Biological Chemistry

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“…The 4214-bp fragment isolated from plasmid pAFFF , digested with AccI and ScaI, was ligated with the 5434-and 5565-bp fragments recovered from plasmids pWFB ATG12 (Rimbault et al, 2000) and pf 307 S (Choquet et al, 2003) digested with the same enzyme to yield plasmids p59dAf K and p59dAf 307 S.…”

Section: Construction Of 59atpa-driven Peta Genesmentioning

confidence: 99%

The Nucleus-Encodedtrans-Acting Factor MCA1 Plays a Critical Role in the Regulation of CytochromefSynthesis inChlamydomonasChloroplasts

et al. 2011

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Organelle gene expression is characterized by nucleus-encoded trans-acting factors that control posttranscriptional steps in a gene-specific manner. As a typical example, in Chlamydomonas reinhardtii, expression of the chloroplast petA gene encoding cytochrome f, a major subunit of the cytochrome b 6 f complex, depends on MCA1 and TCA1, required for the accumulation and translation of the petA mRNA. Here, we show that these two proteins associate in high molecular mass complexes that also contain the petA mRNA. We demonstrate that MCA1 is degraded upon interaction with unassembled cytochrome f that transiently accumulates during the biogenesis of the cytochrome b 6 f complex. Strikingly, this interaction relies on the very same residues that form the repressor motif involved in the Control by Epistasy of cytochrome f Synthesis (CES), a negative feedback mechanism that downregulates cytochrome f synthesis when its assembly within the cytochrome b 6 f complex is compromised. Based on these new findings, we present a revised picture for the CES regulation of petA mRNA translation that involves proteolysis of the translation enhancer MCA1, triggered by its interaction with unassembled cytochrome f.

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“…The N termini of proteins are generally determined, but this method requires protein isolation and is not always conclusive when nascent products are processed at the N terminus. The site-specific disruption of candidate codons by chloroplast transformation was successfully applied to study some of the Chlamydomonas chloroplast genes (20,21). However, this method cannot be applied to genes essential with viability.…”

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confidence: 99%

Termination codon-dependent translation of partially overlapping ndhC-ndhK transcripts in chloroplasts

Yukawa

Sugiura

2008

Proc. Natl. Acad. Sci. U.S.A.

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The chloroplast NAD(P)H dehydrogenase complex, a homologue of mitochondrial complex I, consists of >15 subunits, of which 11 are encoded by the chloroplast genome (ndhA-K). The ndhC and ndhK genes are partially overlapped and cotranscribed in many land plants. The downstream ndhK mRNA possesses 4 possible AUG initiation codons in many dicot plants. By using an efficient in vitro translation system from tobacco chloroplasts, we defined that the major initiation site of tobacco ndhK mRNAs is the third AUG that is located 4 nt upstream from the ndhC stop codon. Mutation of the ndhC stop codon (UAG) arrested translation of the ndhK cistron. Frameshift of the ndhC coding strand inhibited also translation of the distal cistron. The results indicated that ndhK translation depends on termination of the preceding cistron, namely translational coupling. Surprisingly, removal of the ndhC 5-UTR and its AUG still supported substantial translation of the ndhK cistron. This translation was abolished again by removing the ndhC stop codon. Although translation of the downstream cistron of an overlapping mRNA is generally very low, we found that the ndhC/K mRNA produces NdhK and NdhC in similar amounts. Based on subunit compositions of the bacterial complex I, the stoichiometry of NdhK and NdhC is suggested to be 1:1 in chloroplasts. To meet this stoichiometry, the ndhC/K mRNA is translated not only by a translational coupling event but also by a termination codondependent pathway.mRNA ͉ cistron ͉ initiation codon ͉ translational coupling

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Identification of the initiation codon for the atpB gene in Chlamydomonas chloroplasts excludes translation of a precursor form of the β subunit of the ATP synthase

Cited by 18 publications

References 24 publications

The Involvement of Hydrogen-producing and ATP-dependent NADPH-consuming Pathways in Setting the Redox Poise in the Chloroplast of Chlamydomonas reinhardtii in Anoxia

The Involvement of Hydrogen-producing and ATP-dependent NADPH-consuming Pathways in Setting the Redox Poise in the Chloroplast of Chlamydomonas reinhardtii in Anoxia

The Nucleus-Encodedtrans-Acting Factor MCA1 Plays a Critical Role in the Regulation of CytochromefSynthesis inChlamydomonasChloroplasts

Termination codon-dependent translation of partially overlapping ndhC-ndhK transcripts in chloroplasts

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