Identification of the <i>ABO<sup>*</sup>cis-AB04</i> Allele With a Unique Substitution C796A: The First Case in Korea

Yoon, Jung Hee; Youk, Hee Jeong; Chang, Jung Hee; Jang, Minsoo; Choi, Ji Ho; Nam, Mi Hyun; Choi, Ji Seon; Lim, Chae Seung

doi:10.3343/alm.2016.36.6.620

Cited by 5 publications

(3 citation statements)

References 6 publications

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“…Correct ABO typing to the subgroup level is important in that matter, and, although sometimes challenging to novice personnel, serological testing can discriminate cis-AB [13][14][15][16][17][18][19], and other ABO subgroups [20,21] using manual methods (table 3).…”

Section: Discussionmentioning

confidence: 99%

ABO Mistyping of cis-AB Blood Group by the Automated Microplate Technique

Chun

Ryu

Cha

et al. 2018

Transfus Med Hemother

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Background: The cis-AB phenotype, although rare, is the relatively most frequent of ABO subgroups in Koreans. To prevent ABO mistyping of cis-AB samples, our hospital has applied a combination of the manual tile method with automated devices. Herein, we report cases of ABO mistyping detected by the combination testing system. Methods: Cases that showed discrepant results by automated devices and the manual tile method were evaluated. These samples were also tested by the standard tube method. The automated devices used in this study were a QWALYS-3 and Galileo NEO. Exons 6 and 7 of the ABO gene were sequenced. Results: 13 cases that had the cis-AB allele showed results suggestive of the cis-AB subgroup by manual methods, but were interpreted as AB by either automated device. This happened in 87.5% of these cases by QWALYS-3 and 70.0% by Galileo NEO. Genotyping results showed that 12 cases were ABO*cis-AB01/ABO*O01 or ABO*cis-AB01/ABO*O02, and one case was ABO*cis-AB01/ ABO*A102. Conclusion: Cis-AB samples were mistyped as AB by the automated microplate technique in some cases. We suggest that the manual tile method can be a simple supplemental test for the detection of the cis-AB phenotype, especially in countries with relatively high cis-AB prevalence.

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Section: Discussionmentioning

confidence: 99%

ABO Mistyping of cis-AB Blood Group by the Automated Microplate Technique

Chun

Ryu

Cha

et al. 2018

Transfus Med Hemother

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“…The ABO gene encodes a glycosyltransferase, which catalyzes the transfer of carbohydrates to the H antigen, thereby converting the H antigen into A or B antigens, depending on the encoding allele. The common ABO phenotypes in Korea are mostly encoded [1,5,6]. However, ABO*cisAB.05 alleles remain unexplored outside the Chinese population.…”

Section: Introductionmentioning

confidence: 99%

Rare ABO Allele Encoding Glycosyltransferase with Dual Specificity Found in a Cambodian Individual with the A₂B Phenotype

Yu¹,

Chung

Kim

et al. 2020

KJBT

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Cis-AB and B(A) alleles encode an ABO enzyme with dual A and B glycosyltransferase activity. Although globally rare, the cis-AB phenotype is found relatively often in Korean, Japanese, and Chinese populations. Cases of the B(A) allele have been reported mostly in the Chinese population. Forward typing performed in a Cambodian woman with an ABO discrepancy demonstrated a strong reaction with anti-A and anti-B reagents, while there was no reaction with lectin anti-A1. The anti-A1 antibody was detected in reverse typing. Through ABO gene sequence analyses of exons 6 and 7, one of the alleles was identified as ABO*B.01. In contrast, the other allele harboring a c.803G＞C substitution was either ABO*cisAB.05 or ABO*BA.06 allele. The ABO*cisAB.05 and ABO*BA.06 alleles remain indistinguishable despite routine serological testing and ABO genotyping. To the best of the author's knowledge, this is the first case report of these variants discovered in a Cambodian individual residing in Korea.

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“…To date, six B(A) alleles have been identified and characterized, and B(A)02 is even more rare in China compared with B(A)04. Individuals with the CisAB phenotype are characterized by a non-classic mode of inheritance of A and B antigens expressed at the membrane of RBCs: A and B are not transmitted as two independent alleles but as a single allele, referred to as "CisAB" [14,15] . As rare types, B(A) and CisAB have similar performance in serological identification.…”

Section: Introductionmentioning

confidence: 99%

Serological identification and molecular characterization of B (A) 02 subtype in patients and blood donors from Eastern China

Wu¹,

Zhang²,

Guo³

et al. 2017

Blood and Genomics

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This study was designed to identify rare type ABO blood groups, B(A) 02, from Eastern China. Three samples with discordant serological results during routine blood type identification, and four samples from one sample family were selected. All of them were detected by serological method. The exon 6 and 7 of the ABO genes were amplified by PCR and sequenced. They were typed as AsubB by serology and as BO by genotype. In the AsubB samples, nt 700C>G mutation was detected in the B gene, which was previously defined as B(A)02 alleles. In these seven samples, six showed B(A)02/O01 and one showed B(A)02/O02. B(A)02 allele was found to be more common in this study than B(A)04, which is considered to be more frequent than B(A)02. The careful identification of rare blood types is important for the safety of clinical blood transfusion.

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Identification of the ABO^cis-AB04* Allele With a Unique Substitution C796A: The First Case in Korea

Cited by 5 publications

References 6 publications

ABO Mistyping of cis-AB Blood Group by the Automated Microplate Technique

ABO Mistyping of cis-AB Blood Group by the Automated Microplate Technique

Rare ABO Allele Encoding Glycosyltransferase with Dual Specificity Found in a Cambodian Individual with the A₂B Phenotype

Serological identification and molecular characterization of B (A) 02 subtype in patients and blood donors from Eastern China

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Identification of the ABO*cis-AB04 Allele With a Unique Substitution C796A: The First Case in Korea

Cited by 5 publications

References 6 publications

ABO Mistyping of cis-AB Blood Group by the Automated Microplate Technique

ABO Mistyping of cis-AB Blood Group by the Automated Microplate Technique

Rare ABO Allele Encoding Glycosyltransferase with Dual Specificity Found in a Cambodian Individual with the A2B Phenotype

Serological identification and molecular characterization of B (A) 02 subtype in patients and blood donors from Eastern China

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Identification of the ABO^cis-AB04* Allele With a Unique Substitution C796A: The First Case in Korea

Rare ABO Allele Encoding Glycosyltransferase with Dual Specificity Found in a Cambodian Individual with the A₂B Phenotype