Identification of the human sperm protein that interacts with sperm-immobilizing antibodies in the sera of infertile women

Kamada, Masao; Yamamoto, Shozo; Takikawa, Masaya; Kunimi, Kotaro; Murakami, Masahiko; Futaki, Shiroh; Ohmoto, Yasukazu; Aono, Toshihiro; Koide, S. S.

doi:10.1016/s0015-0282(99)00314-3

Cited by 7 publications

(3 citation statements)

References 18 publications

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“…On the other hand, women can also produce anti sperm-antibodies. One possible target for such antibodies is the human sperm protein rSMP-B [102]. These antibodies can coagulate and immobilize the spermatozoa or can even be cytotoxic [103] and eventually lead to infertility as well as reduced fertilization rates in assisted reproduction [104].…”

Section: Immunological Infertility and Artmentioning

confidence: 99%

Sperm preparation for ART

Henkel

Schill

2003

Reprod Biol Endocrinol

417

175

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The onset of clinical assisted reproduction, a quarter of a century ago, required the isolation of motile spermatozoa. As the indication of assisted reproduction shifted from mere gynaecological indications to andrological indications during the years, this urged andrological research to understand the physiology of male germ cell better and develop more sophisticated techniques to separate functional spermatozoa from those that are immotile, have poor morphology or are not capable to fertilize oocytes. Initially, starting from simple washing of spermatozoa, separation techniques, based on different principles like migration, filtration or density gradient centrifugation evolved. The most simple and cheapest is the conventional swim-up procedure. A more sophisticated and most gentle migration method is migration-sedimentation. However, its yield is relatively small and the technique is therefore normally only limited to ejaculates with a high number of motile spermatozoa. Recently, however, the method was also successfully used to isolate spermatozoa for intracytoplasmic sperm injection (ICSI). Sperm separation methods that yield a higher number of motile spermatozoa are glass wool filtration or density gradient centrifugation with different media. Since Percoll® as a density medium was removed from the market in 1996 for clinical use in the human because of its risk of contamination with endotoxins, other media like IxaPrep®, Nycodenz, SilSelect®, PureSperm® or Isolate® were developed in order to replace Percoll®. Today, an array of different methods is available and the selection depends on the quality of the ejaculates, which also includes production of reactive oxygen species (ROS) by spermatozoa and leukocytes. Ejaculates with ROS production should not be separated by means of conventional swim-up, as this can severely damage the spermatozoa. In order to protect the male germ cells from the influence of ROS and to stimulate their motility to increase the yield, a number of substances can be added to the ejaculate or the separation medium. Caffeine, pentoxifylline and 2-deoxyadenosine are substances that were used to stimulate motility. Recent approaches to stimulate spermatozoa include bicarbonate, metal chelators or platelet-activating factor (PAF). While the use of PAF already resulted in pregnancies in intrauterine insemination, the suitability of the other substances for the clinical use still needs to be tested. Finally, the isolation of functional spermatozoa from highly viscous ejaculates is a special challenge and can be performed enzymatically to liquefy the ejaculate. The older method, by which the ejaculate is forcefully aspirated through a narrow-gauge needle, should be abandoned as it can severely damage spermatozoa, thus resulting in immotile sperm.

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Section: Immunological Infertility and Artmentioning

confidence: 99%

Sperm preparation for ART

Henkel

Schill

2003

Reprod Biol Endocrinol

417

175

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“…To show clinical relevance of these animal studies, ASAs present in the blood of infertile women were assessed for their capacity to interact with rSMP‐230. A noteworthy finding was that a significant number of sera (10 out of 29), obtained from infertile women containing antibodies with sperm immobilizing activity, interacted with rSMP‐230, a segment of rSMP‐B protein (101). Polyclonal antibodies to rSMP‐230 also immobilize human sperm in vitro and block the binding of human sperm to zona membranes.…”

Section: Sperm Tail Component (Rsmp‐b)mentioning

confidence: 99%

Antisperm Antibodies Associated with Infertility: Properties and Encoding Genes of Target Antigens

Koide

Wang

Kamada

2000

Proc Soc Exp Biol Med

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Abstract. Infertility among couples of reproductive age is a perplexing condition when the cause is indeterminate. These cases are classified as unexplained infertility. In a subset of subjects, antisperm antibodies with sperm agglutinating and/or immobilizing activities have been detected in the blood or fluids of the reproductive tract. These cases are designated as immunologic infertility although a cause and effect relationship of the antibodies to infertility has not been established. In this review, seven target sperm antigens to antibodies associated with infertility and their encoding genes are described. The antisperm antibodies (ASAs) examined were obtained from infertile women or were monoclonal antibodies (mAb) raised against human sperm proteins. All the ASAs studied possessed potent sperm agglutinating and/or immobilizing activities. The target antigens were isolated from human and other mammalian sperm, and the encoding genes identified. The seven antigens are YWK‐II, BE‐20, rSMP‐B, BS‐63 (nucleoporin‐related), BS‐17 (calpastatin), HED‐2 (zyxin), and 75‐ kDa. Each antigen is a distinct and separate entity and is produced by different cells of the reproductive tract, (e.g., germ cells, epididymal epithelial cells, and Sertoli cells). No single predominant target component has been found to interact with the ASAs. It is proposed that immunologic infertility is the consequence of the combined actions of multiple ASAs in immobilizing and/or agglutinating spermatozoa, blocking spermegg interaction, preventing implantation, and/or arresting embryo development.

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“…1,14 The oocyte defects may result from an abnormal ZP that is unable to induce the acrosome reaction, abnormal cortical granule exocytosis, or autoantibodies to ZP. [15][16][17][18][19][20][21] It is well established that optimal sperm-ZP binding is important for normal fertilization 22 and that defective sperm-ZP interaction is a major cause of fertilization failure in clinical IVF. 23 Several in vitro tests to determine sperm-ZP binding and penetration have been developed and the results correlate well with fertilization outcome.…”

Section: Female Infertilitymentioning

confidence: 99%

Human Sperm-Oocyte Recognition and Infertility

Prasad¹,

Dunbar²

2000

Semin Reprod Med

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The incidence of infertility related to both male and female factors continues to rise despite many advances in reproductive technologies. Some abnormalities in human gamete interaction have been shown to be due to defects in the sperm, and others have been attributed to defects in the zona pellucida (ZP). Our lack of understanding of molecular mechanisms involved in the interaction of human sperm with the ZP in fertile as compared with infertile females and males has been limited because of the unavailability of human oocytes and ethical restraints on experimental studies. It is becoming increasingly apparent that improved clinical assays are necessary for evaluating sperm-ZP interaction in order to assess the optimal procedures for successful fertilization and pregnancy. With advances in molecular biology, the genes encoding the three major human ZP proteins have been identified and complementary DNAs are available to begin to better evaluate the molecular basis of sperm-ZP interaction.

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Identification of the human sperm protein that interacts with sperm-immobilizing antibodies in the sera of infertile women

Cited by 7 publications

References 18 publications

Sperm preparation for ART

Sperm preparation for ART

Antisperm Antibodies Associated with Infertility: Properties and Encoding Genes of Target Antigens

Human Sperm-Oocyte Recognition and Infertility

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