Identification of the Genetic Requirements for Zinc Tolerance and Toxicity in<i>Saccharomyces cerevisiae</i>

Zhao, Yang; Cao, Can; Liu, Yingli; Wang, Jing; Li, Jie; Li, Shiyun; Deng, Yu

doi:10.1534/g3.119.400933

Cited by 25 publications

(26 citation statements)

References 61 publications

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“…Some metal-containing compounds are also thought to act by inducing oxidative stress, and our data analysis showed that most of the metals in our survey (cadmium, copper, chromium, and aluminum) caused accumulation of reactive oxygen species. Zinc was not tested for ROS formation in a cell death context, but genome wide screening for resistance to zinc did show that numerous mutants formed ROS in its presence [163]. Only manganese stress did not cause ROS formation, though in one paper [164] its toxicity was dependent on the mitochondria, which might implicate ROS.…”

Section: Oxidantsmentioning

confidence: 99%

A Systematic Survey of Characteristic Features of Yeast Cell Death Triggered by External Factors

Grosfeld

Bidiuk

Mitkevich

et al. 2021

JoF

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Cell death in response to distinct stimuli can manifest different morphological traits. It also depends on various cell death signaling pathways, extensively characterized in higher eukaryotes but less so in microorganisms. The study of cell death in yeast, and specifically Saccharomyces cerevisiae, can potentially be productive for understanding cell death, since numerous killing stimuli have been characterized for this organism. Here, we systematized the literature on external treatments that kill yeast, and which contains at least minimal data on cell death mechanisms. Data from 707 papers from the 7000 obtained using keyword searches were used to create a reference table for filtering types of cell death according to commonly assayed parameters. This table provides a resource for orientation within the literature; however, it also highlights that the common view of similarity between non-necrotic death in yeast and apoptosis in mammals has not provided sufficient progress to create a clear classification of cell death types. Differences in experimental setups also prevent direct comparison between different stimuli. Thus, side-by-side comparisons of various cell death-inducing stimuli under comparable conditions using existing and novel markers that can differentiate between types of cell death seem like a promising direction for future studies.

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Section: Oxidantsmentioning

confidence: 99%

A Systematic Survey of Characteristic Features of Yeast Cell Death Triggered by External Factors

Grosfeld

Bidiuk

Mitkevich

et al. 2021

JoF

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“…The first-strand cDNA synthesis was performed using the Primer Script RT reagent kit (Cwbiotech, China) according to the manufacturer's instructions. The expression mRNA levels of TRR1, TRX2, GSH1, SOD1, CTT1 and GPX2 were detected by quantitative PCR (qPCR) as described previously [57] (Additional file 1: Table S1). Each reaction was carried out in triplicate.…”

Section: Rna Extraction and Quantitative Pcr Analysismentioning

confidence: 99%

Genome-wide identification for genes involved in sodium dodecyl sulfate toxicity in Saccharomyces cerevisiae

Cao

et al. 2020

BMC Microbiol

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Background: Sodium dodecyl sulfate (SDS) is one of the most widely used anionic alkyl sulfate surfactants. Toxicological information on SDS is accumulating, however, mechanisms of SDS toxicity regulation remain poorly understood. In this study, the relationship between the SDS-sensitive mutants and their intracellular ROS levels has been investigated. Results: Through a genome-scale screen, we have identified 108 yeast single-gene deletion mutants that are sensitive to 0.03% SDS. These genes were predominantly related to the cellular processes of metabolism, cell cycle and DNA processing, cellular transport, transport facilities and transport routes, transcription and the protein with binding function or cofactor requirement (structural or catalytic). Measurement of the intracellular ROS (reactive oxygen species) levels of these SDS-sensitive mutants showed that about 79% of SDS-sensitive mutants accumulated significantly higher intracellular ROS levels than the wild-type cells under SDS stress. Moreover, SDS could generate oxidative damage and up-regulate several antioxidant defenses genes, and some of the SDSsensitive genes were involved in this process. Conclusion: This study provides insight on yeast genes involved in SDS tolerance and the elevated intracellular ROS caused by SDS stress, which is a potential way to understand the detoxification mechanisms of SDS by yeast cells.

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“…Therefore, the high concentration of iron in the medium may have increased the intracellular labile iron pool, which in turn generated ROS. Excess zinc can also cause an increase in ROS accumulation, and disrupt iron metabolism, such as Fe-S cluster synthesis, in S. cerevisiae [ 29 , 30 ]. Excess copper also generates ROS and causes oxidative stress, DNA damage, and membrane disruption in S. cerevisiae [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress Causes Vacuolar Fragmentation in the Human Fungal Pathogen Cryptococcus neoformans

Kim

Song

et al. 2021

JoF

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Vacuoles are dynamic cellular organelles, and their morphology is altered by various stimuli or stresses. Vacuoles play an important role in the physiology and virulence of many fungal pathogens. For example, a Cryptococcus neoformans mutant deficient in vacuolar functions showed significantly reduced expression of virulence factors such as capsule and melanin synthesis and was avirulent in a mouse model of cryptococcosis. In the current study, we found significantly increased vacuolar fragmentation in the C. neoformans mutants lacking SOD1 or SOD2, which respectively encode Zn, Cu-superoxide dismutase and Mn-superoxide dismutase. The sod2 mutant showed a greater level of vacuole fragmentation than the sod1 mutant. We also observed that the vacuoles were highly fragmented when wild-type cells were grown in a medium containing high concentrations of iron, copper, or zinc. Moreover, elevated temperature and treatment with the antifungal drug fluconazole caused increased vacuolar fragmentation. These conditions also commonly cause an increase in the levels of intracellular reactive oxygen species in the fungus, suggesting that vacuoles are fragmented in response to oxidative stress. Furthermore, we observed that Sod2 is not only localized in mitochondria but also in the cytoplasm within phagocytosed C. neoformans cells, possibly due to copper or iron limitation.

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Identification of the Genetic Requirements for Zinc Tolerance and Toxicity inSaccharomyces cerevisiae

Cited by 25 publications

References 61 publications

A Systematic Survey of Characteristic Features of Yeast Cell Death Triggered by External Factors

A Systematic Survey of Characteristic Features of Yeast Cell Death Triggered by External Factors

Genome-wide identification for genes involved in sodium dodecyl sulfate toxicity in Saccharomyces cerevisiae

Oxidative Stress Causes Vacuolar Fragmentation in the Human Fungal Pathogen Cryptococcus neoformans

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