Identification of the genetic locus associated with the crinkled leaf phenotype in a soybean (Glycine max L.) mutant by BSA-Seq technology

OCHAR, Kingsley; Su, Bohong; ZHOU, Ming-ming; LIU, Zhang-xiong; Gao, Huawei; Lamlom, Sobhi F.; Qiu, Lijuan

doi:10.1016/j.jia.2022.08.095

Cited by 15 publications

(8 citation statements)

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“…In this study, the candidate SNP was isolated using MutMap, and the sequence differences between the two allelic pools were based on the previous study [38], which demonstrated this SNP's efficiency and accuracy in other crops such as rice (Oryza sativa) [28], soybean (Glycine max) [10] and Setaria italic [39]. In our study, sequencing verification and homologous comparison results showed that a Gly to Ser amino acid substitution in a highly conserved region of Sobic.003G010300 (Figure 3) was responsible for the low biomass yield and lobed-leaf phenotype of the sblob mutant.…”

Section: Single Nucleotide Mutations Results In Gene Functional Inact...mentioning

confidence: 99%

“…In cereal crops, NARROW LEAF 1 and NARROW LEAF 7 regulate the leaf morphology of rice via the auxin-mediated acid growth mechanism [12,18]. GLYCINE MAX DWARF CRINKLED LEAF 1 (GmDCL1) is a promising gene involved in the morphogenesis of the crinkled leaf trait of the soybean [10]. These studies demonstrate that leaf morphology is a complex trait involved in many genetic pathways such as hormone signals, transcription factors and the interactions between them [15].…”

Section: Introductionmentioning

confidence: 95%

“…Leaf morphology is one of the key agronomic traits affecting the biomass yield of sorghum, presenting a broad range of phenotypic variations relevant to its use as a potential energy crop [8]. It can also be used to determine the efficiency of photosynthesis, plant architecture and nutrient distribution [9,10], as larger leaf areas have been positively correlated with biomass yield characteristics in sorghum [11]. Moreover, leaf morphology can also affect nutrient uptake, which is crucial to the plant's growth and biomass yield [12].…”

Section: Introductionmentioning

confidence: 99%

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Mapping and Screening of Candidate Gene Regulating the Biomass Yield of Sorghum (Sorghum bicolor L.)

Li,

Cai,

Liang

et al. 2024

IJMS

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Biomass yield is one of the important traits of sorghum, which is greatly affected by leaf morphology. In this study, a lobed-leaf mutant (sblob) was screened and identified, and its F2 inbred segregating line was constructed. Subsequently, MutMap and whole-genome sequencing were employed to identify the candidate gene (sblob1), the locus of which is Sobic.003G010300. Pfam and homologous analysis indicated that sblob1 encodes a Cytochrome P450 protein and plays a crucial role in the plant serotonin/melatonin biosynthesis pathway. Structural and functional changes in the sblob1 protein were elucidated. Hormone measurements revealed that sblob1 regulates both leaf morphology and sorghum biomass through regulation of the melatonin metabolic pathway. These findings provide valuable insights for further research and the enhancement of breeding programs, emphasizing the potential to optimize biomass yield in sorghum cultivation.

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Section: Single Nucleotide Mutations Results In Gene Functional Inact...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Mapping and Screening of Candidate Gene Regulating the Biomass Yield of Sorghum (Sorghum bicolor L.)

Li,

Cai,

Liang

et al. 2024

IJMS

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“…Cleaved amplified polymorphic sequences (CAPS) markers are commonly used to detect SNP sites in wheat breeding because of their specificity, simple operation and low cost (Collard et al, 2005; Dean et al, 2012; Shavrukov, 2016). At present, CAPS functional markers are widely used in wheat, maize, rice, soybean, and other crops (Zhang et al, 2010; Dissanayaka et al, 2014; Zhang et al, 2017; Ochar et al, 2022). In wheat, the development of functional markers provides theoretical basis for rapid and high-quality breeding, such as the genes Rht-B1b and Rht-D1b , PPO , and TaCwi-A1 (Ellis et al, 2002; He et al, 2007; Ma et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Functional marker CAPS-799 of theTaPHT1;9-4Bgene is useful for screening phosphorus-efficient wheat cultivars

Wang,

Chen,

Shi

et al. 2024

Preprint

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Context: In our previous study, TaPHT1;9-4B, one key high-affinity Pi transporter, was found to greatly contribute to Pi acquisition and transportation, and its functional marker CAPS-799 was subsequently developed to identify its Pi-efficient elite haplotype. Objective: The study aimed to screen a varieties of wheat cultivars by using the above CAPS-799, identify its Pi-efficient elite haplotype cultivars, and reveal its physiological mechanism. Methods: Successive two-year field experiments without Pi fertilizer supply, and hydroponic experiment with low Pi (10 μM) were performed. P concentrations, biomasses, grain yields, yield components, root growth parameters, and TaPHT1;9-4B transcript levels were measured. Total P accumulation and transport efficiency, and the relative growth rates were calculated. Results: Eight Pi-efficient wheat cultivars (Hap3 haplotype) were screened out by using the CAPS-799 from 80 modern major cultivars, and in successive two-year field experiments, their grain yields, spike numbers, P absorption and transport efficiencies were significantly higher than those of Non-Hap3 haplotypes (Hap1, 2 and 4) under no Pi fertilizer supply condition, and therefore, these eight cultivars belonged to Pi-efficient elite haplotype. TaPHT1;9-4B transcript levels in roots at the early stage of grain filling period in field experiment, and the relative growth rates of total root surface areas, volumes and mean root diameters of Hap3 cultivars in hydroponic experiment, were markedly higher than other haplotypes. Conclusions: CAPS-799 was a useful functional marker for screening Pi-efficient wheat cultivars, and its Pi-efficient wheat cultivars were characterized with higher TaPHT1;9-4B transcript levels and more roots. Implications: CAPS-799 will be used to screen or develop Pi-efficient wheat cultivars.

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“…Therefore, the lack of suitable genetic diversity in breeding germplasm is a major constraint slowing cotton breeders' progress in developing new cultivars [ 15 – 19 ]. Using molecular markers as an alternative tool to identify and select superior parents early in breeding programs and incorporate them into marker-assisted selection (MAS) could significantly enhance genetics and reduce time and costs required to develop novel cotton cultivars [ 12 , 20 – 22 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring agro-morphological and fiber traits diversity in cotton (G. barbadense L.)

Salama,

Farid,

El-Mahalawy

et al. 2024

BMC Plant Biol

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Cotton (Gossypium barbadense L.) is a leading fiber and oilseed crop globally, but genetic diversity among breeding materials is often limited. This study analyzed genetic variability in 14 cotton genotypes from Egypt and other countries, including both cultivated varieties and wild types, using agro-morphological traits and genomic SSR markers. Field experiments were conducted over two seasons to evaluate 12 key traits related to plant growth, yield components, and fiber quality. Molecular diversity analysis utilized 10 SSR primers to generate DNA profiles. The Molecular diversity analysis utilized 10 SSR primers to generate DNA profiles. Data showed wide variation for the morphological traits, with Egyptian genotypes generally exhibiting higher means for vegetative growth and yield parameters. The top-performing genotypes for yield were Giza 96, Giza 94, and Big Black Boll genotypes, while Giza 96, Giza 92, and Giza 70 ranked highest for fiber length, strength, and fineness. In contrast, molecular profiles were highly polymorphic across all genotypes, including 82.5% polymorphic bands out of 212. Polymorphism information content was high for the SSR markers, ranging from 0.76 to 0.86. Genetic similarity coefficients based on the SSR data varied extensively from 0.58 to 0.91, and cluster analysis separated genotypes into two major groups according to geographical origin. The cotton genotypes displayed high diversity in morphology and genetics, indicating sufficient variability in the germplasm. The combined use of physical traits and molecular markers gave a thorough understanding of the genetic diversity and relationships between Egyptian and global cotton varieties. The SSR markers effectively profiled the genotypes and can help select ideal parents for enhancing cotton through hybridization and marker-assisted breeding.

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Identification of the genetic locus associated with the crinkled leaf phenotype in a soybean (Glycine max L.) mutant by BSA-Seq technology

Cited by 15 publications

References 77 publications

Mapping and Screening of Candidate Gene Regulating the Biomass Yield of Sorghum (Sorghum bicolor L.)

Mapping and Screening of Candidate Gene Regulating the Biomass Yield of Sorghum (Sorghum bicolor L.)

Functional marker CAPS-799 of theTaPHT1;9-4Bgene is useful for screening phosphorus-efficient wheat cultivars

Exploring agro-morphological and fiber traits diversity in cotton (G. barbadense L.)

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