Identification of the gene encoding granule-bound starch synthase I in sweet potato (Ipomoea batatas (L.) Lam.).

Abstract

The cDNA and genomic clones from sweet potato encoding granule-bound starch synthase I (GBSSI) were isolated and characterized. The sequence analysis of the cDNA shows that the sweet potato GBSSI mature protein is comprised of 531 amino acids and that the precursor has a transit peptide of 77 amino acids.

Molecular cloning and characterization of granule bound starch synthase I cDNA from a grain amaranth (Amaranthus cruentus L.)

“…The peptide was similar in size to the GBSSI transit peptide of sweet potato (Kimura et al 2000), potato (van der Leij et al 1991), and cassava (Salehuzzaman et al 1993). The transit peptide of amaranth has 43.7% identity over amino acids with that of the sweet potato but much less with other plant species (14.1~32.9%) ( Table 4).…”

“…3). This structure is similar to that of the nonwaxy type characterized in other species such as maize (Klosgen et al Table 3. 1986), rice (Okagaki 1992), foxtail millet (Fukunaga et al 2002), potato (van der Leij et al 1991, and sweet potato (Kimura et al 2000). All five-plant species contain 14 exons, with exons 2-14 (13 exons) contributing to the coding sequence.…”

“…To date, sequence information of the GBSSI structural genes from several species, such as maize (Klosgen et al 1986), rice (Okagaki 1992), barley (Rohde et al 1988), wheat (Clark et al 1991, Murai et al 1999, potato (van der Leij et al 1991), sweet potato (Kimura et al 2000) and foxtail millet (Fukunaga et al 2002) have been published. To facilitate further studies of amaranth starch, it is essential to characterize the GBSSI gene and to establish its primary structures.…”

Molecular characterization and comparative analysis of two waxy alleles in barley

“…To date, the waxy structural genes have been characterized from wheat (Murai et al, 1999), maize (Klösgeni et al, 1986), rice (Wang et al, 1990;Okagaki, 1992), barley (Rohde et al, 1988), rye (Mason-Gamer, 2001;Xu et al, 2009), sorghum (Hsieh et al, 1996), potato (van der Leij et al, 1991), pea (Dry et al, 1992), cassava (Salehuzzaman et al, 1993) potato (Kimura et al, 2000), foxtail millet (Fukunaga et al, 2002) and amaranth (Park et al, 2009a). The loss of GBSS function in cereal crops can produce the waxy phenotype which is featured by the altered texture and appearance of starchy endosperms (Denyer et al, 2001).…”

Identification and characterization of granule bound starch synthase I (GBSSI) gene of tartary buckwheat (Fagopyrum tataricum Gaertn.)

“…Since low or no amylose production leads to waxy endosperm, the GBSSI gene is also known as waxy gene. Because of the importance of GBSSI gene to influence starch quality, it has been characterized and studied in many crop plants, including cereal grain plants of the family Poaceae, such as rice (Sano, 1984), maize (Tsai, 1974), barley (Patron et al, 2002), bread and durum wheat (Fujita et al, 2001;Lafiandra et al, 2010;Miura et al, 1994;Nakamura et al, 1995) and foxtail millet (Fukunaga et al, 2002), tuber plants, such as potato (van der Leij et al, 1991), sweet potato (Kimura et al, 2000) and cassava (Salehuzzaman et al, 1993), and some important food crops and herb crops, e.g. peas (Edwards, 2002).…”