Identification of the GABAA receptor α-subunit mRNA in rat intestine

Zeiter, Donna K.; Li, Xinshe; Broussard, Delma L.

doi:10.1016/0169-328x(96)00077-0

Cited by 17 publications

(13 citation statements)

References 14 publications

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“…Muscimol opens chloride channels, resulting in neuron hyperpolarization and stability. Several other studies have shown that GABA A receptors are abundant on myenteric plexus neurons [108][109][110][111]. Majewska et al [112] have also demonstrated that the progesterone metabolite, 3·-hydroxy-5·-pregnan-20-one, has a potent barbiturate-like ligand effect on the GABA-barbiturate receptor complex.…”

Section: Progesteronementioning

confidence: 95%

Relationship of Reproductive Hormones and Neuromuscular Disease of the Gastrointestinal Tract

Mathias¹,

Clench²

1998

Dig Dis

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Functional disorders of the gastrointestinal tract comprise a common but ill-defined group of diseases; they primarily afflict women. Although predominantly involving nerve and muscle, the cellular and molecular bases of the pathogenesis of these functional disorders are unknown. Clinical studies indicate that some result from neural dysfunction within the enteric nervous system, others may be due to muscular problems, and the causes of still others remain unknown. Laboratory studies have shown that ovarian products such as progesterone, luteinizing hormone, human chorionic gonadotropin, and relaxin (but not estrogen), are neural antagonists of gastrointestinal motility. The production and secretion of these ovarian substances are controlled by gonadotropin-releasing hormone (GnRH) released from the hypothalamus; they probably act on γ-aminobutyric acid receptors and alter chloride influx into the cell. GnRH analogs are effective drugs that downmodulate the hypothalamic-pituitary-gonadal axis and inhibit the secretion of gonadal products involved in such hormone-dependent diseases as endometriosis and prostate cancer. Acting on the GnRH receptors (seven transmembrane domain receptors) on myenteric neurons, GnRH analogs are also effective neural modulators in such disorders as functional bowel disease. These analogs are a promising new group of compounds that may be used to treat difficult gastrointestinal problems.

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Section: Progesteronementioning

confidence: 95%

Relationship of Reproductive Hormones and Neuromuscular Disease of the Gastrointestinal Tract

Mathias¹,

Clench²

1998

Dig Dis

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“…Electrophysiological and biochemical data suggest that GABA receptors are located on somas of AH neurones and GABA receptors are found on the nerve terminals of both AH-and S-type neurones (Ong & Kerr, 1987;Hills et al, 1987;Gentilini et al, 1992;Krantis et al, 1994). The results of pharmacological studies indicate that thesubunit of the GABA receptor is coupled to the chloride channel (Cherubini & North, 1984;Taniyama et al, 1988;Zeiter et al, 1996).…”

Section: Numerical Simulation Of Motility Patterns Of the Small Bowelmentioning

confidence: 98%

“…GABA receptors are coupled to ligand-gated L-type Ca> and Cl\ channels on smooth muscle (Ong & Kerr 1987;Napoleone et al, 1991;Gentilini et al, 1992;Krantis et al, 1994;Zeiter et al, 1996).…”

Section: Fig 12mentioning

confidence: 98%

Numerical Simulation of Motility Patterns of the Small Bowel. II. Comparative Pharmacological Validation of a Mathematical Model

Miftakhov

Abdusheva

Christensen

1999

Journal of Theoretical Biology

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“…A subset of enteric neurons contain GABA, and both glutamic acid decarboxylase and GABA transaminase are in the enteric nervous system (Miki et al 1983;Saito and Tanaka 1986;Jessen et al 1986Jessen et al , 1987Furness et al 1989;Williamson et al 1996;Krantis et al 1998). Moreover, there is anatomical and physiological evidence for GABA A , GABA B and GABA C receptors in the gastrointestinal tract (Fargeas et al 1988;Bertrand and Galligan 1992;Gentilini et al 1992;Krantis et al 1995;Zeiter et al 1996;Poulter et al 1999;Fletcher et al 2001). It would be expected that a specific GABA uptake system is also present, and indeed GABA uptake into enteric glia and neurons has been demonstrated (Jessen et al 1979;Kerr and Krantis 1983;Saffrey et al 1983;Furness et al 1989), although GABA transporters have not been specifically identified.…”

Section: Introductionmentioning

confidence: 95%

Neuronal and glial localization of GABA transporter immunoreactivity in the myenteric plexus

Fletcher

Clark

Furness

2002

Cell and Tissue Research

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The neurotransmitter gamma-aminobutyric acid (GABA) is removed from the extracellular space by sodium and chloride dependent high affinity plasma membrane transporters. In the rat central nervous system, three GABA transporters, GAT1, GAT2 and GAT3, have been cloned and localized by immunohistochemistry. The purpose of this study was to examine the distribution of these transporters within the myenteric plexus of the rat gastrointestinal tract. We investigated their cellular locations using GAT1-3 specific antisera in lightly fixed segments of rat duodenum, ileum and colon. Immunohistochemistry revealed a large number of GAT2-immunoreactive structures that surrounded neurons within each ganglion of the myenteric plexus. GAT2 was colocalized in these structures with the glial cell marker p75(NTR), suggesting that the predominant high affinity GABA transporter within enteric glia is GAT2. GAT3 immunoreactivity was localized within many nerve cell bodies, and no labeling for GAT1 was detected, although it was present in retina, which was used as a control. Double labeling for calretinin and nitric oxide synthase (NOS) revealed colocalization of GAT3 with approximately 75% of calretinin-immunoreactive neurons and 15% of NOS-immunoreactive neurons. This suggests that a small proportion of inhibitory motor neurons and at least some putative intrinsic primary afferent neurons within the rat gastrointestinal tract express GAT3. Thus NOS neurons, which appear to utilize GABA as a transmitter, and calretinin-immunoreactive neurons, which do not appear to be GABAergic, both express immunoreactivity for GABA transporters.

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Identification of the GABAA receptor α-subunit mRNA in rat intestine

Cited by 17 publications

References 14 publications

Relationship of Reproductive Hormones and Neuromuscular Disease of the Gastrointestinal Tract

Relationship of Reproductive Hormones and Neuromuscular Disease of the Gastrointestinal Tract

Numerical Simulation of Motility Patterns of the Small Bowel. II. Comparative Pharmacological Validation of a Mathematical Model

Neuronal and glial localization of GABA transporter immunoreactivity in the myenteric plexus

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