Identification of the flagellar chaperone FlgN in the phytopathogen Xanthomonas axonopodis pathovar citri by its interaction with hook-associated FlgK

Abstract

Genome annotation of the plant pathogen Xanthomonas axonopodis pv. citri (Xac), identified flagellar genes in a 15.7 kb gene cluster. However, FlgN, a secretion chaperone for hook-associated proteins FlgK and FlgL, was not identified. We performed extensive screening of the X. axonopodis pv. citri genome with the yeast two-hybrid system to identify a protein with the characteristics of the flagellar chaperone FlgN. We found a candidate (XAC1990) encoded by an operon for components of the flagellum apparatus that interacted with FlgK. In order to further support this finding, Xac FlgK and XAC1990 were cloned, expressed, and purified. The recombinant proteins were characterized by spectroscopic methods and their interaction in vitro confirmed by pull-down assays. We, therefore, conclude that XAC1990 and its homologs in other Xanthomonas species are, in fact, FlgN proteins. These observations extend the sequence diversity covered by this family of proteins.

Flagella and Pili of Xanthomonas axonopodis pv. glycines are associated with motility, biofilm formation and virulence on soybean

“…oryzae (). The remaining two clusters are composed of 14 ( fliRQPONMLKJIHGFE ) and 17 genes ( fliSDC , flgLKJIHGFEDCB , cheV , fIgAM ) (Khater et al, ). It has been reported that some flagella genes such as fliC in X. campestris pv.…”

The Xanthomonas axonopodis pv. citri flagellum is required for mature biofilm and canker development

“…Flagellar gene clusters encode all the structural proteins of the flagellum in Xac (da Silva et al, 2002). In addition, it has been shown that several genes encoding auxiliary protein subunits are involved in regulation of the flagellar assembly (Khater et al, 2007). As in many other bacterial species, FliC and FlgE proteins are the flagellin (Vonderviszt et al, 1998) and the hook components (Aizawa, 1996), respectively, of the flagellar structure.…”

Orange waste as a biomass for 2G-ethanol production using low cost enzymes and co-culture fermentation

“…citri (Xac) proteins, a virulent Gram-negative pathogen that causes citrus canker disease. [11][12][13] These bacteria enter into the host-plant through the stomata, and through an impressive arsenal of proteins, including pectinases and celullases, cause visible circular spot-wounds. 14 Several bacteria and fungi have been reported to produce cocktails of hydrolytic depolymerising lignocellulosic enzymes and usually fungi are considered a better option in terms of enzyme varieties and yields.…”