Identification of the first neuropeptides from the enigmatic hexapod order Protura

Christie, Andrew E.; Chi, Megan

doi:10.1016/j.ygcen.2015.05.015

Cited by 18 publications

(5 citation statements)

References 60 publications

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“…In contrast, the evidence we found for GSEFLamide-encoding genes/transcripts in hexapods was limited to orders within the ametabolous class Entognatha, i.e. the Collembola (springtails), Diplura (bristletails) and Protura [coneheads (Christie and Chi, 2015a; this study)], and the most basal orders within the Insecta, i.e. the ametabolous Zygentoma (silverfish), and the hemimetabolous Odonata (dragonflies and damselflies) and Ephemeroptera (mayflies).…”

Section: Gseflamides Appear Broadly Conserved Within the Arthropoda contrasting

confidence: 63%

“…Prior genomic/ transcriptomic analyses suggested that the GSEFLamides are broadly conserved within the Arthropoda, with evidence for their existence being found in one or more species from each subphylum, i.e. Chelicerata (Christie, 2015a;Christie and Chi, 2015b;Veenstra et al, 2012), Myriapoda (Christie, 2015c), Crustacea (Bao et al, 2015;Christie, 2014aChristie, ,c,f, 2015bChristie and Chi, 2015c;Christie and Pascual, 2016;Christie et al, 2015Christie et al, , 2017bChristie et al, , 2018aNguyen et al, 2016;Toullec et al, 2017) and Hexapoda (Christie and Chi, 2015a;Christie et al, 2017a). However, the number of studies that have reported putative GSEFLamide-encoding genes/transcripts is limited, especially for the Myriapoda and Hexapoda; thus, precisely how conserved this peptide family is within the phylum/subphyla remains unresolved.…”

Section: Conservation Of the Gseflamide Family In The Arthropodamentioning

confidence: 99%

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AMGSEFLamide, a member of a broadly conserved peptide family, modulates multiple neural networks inHomarus americanus

Dickinson

Oleisky

Rivera

et al. 2018

Journal of Experimental Biology

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Recent genomic/transcriptomic studies have identified a novel peptide family whose members share the carboxyl terminal sequence-GSEFLamide. However, the presence/identity of the predicted isoforms of this peptide group have yet to be confirmed biochemically, and no physiological function has yet been ascribed to any member of this peptide family. To determine the extent to which GSEFLamides are conserved within the Arthropoda, we searched publicly accessible databases for genomic/transcriptomic evidence of their presence. GSEFLamides appear to be highly conserved within the Arthropoda, with the possible exception of the Insecta, in which sequence evidence was limited to the more basal orders. One crustacean in which GSEFLamides have been predicted using transcriptomics is the lobster, Homarus americanus. Expression of the previously published transcriptome-derived sequences was confirmed by reverse transcription (RT)-PCR of brain and eyestalk ganglia cDNAs; mass spectral analyses confirmed the presence of all six of the predicted GSEFLamide isoforms-IGSEFLamide, MGSEFLamide, AMGSEFLamide, VMGSEFLamide, ALGSEFLamide and AVGSEFLamidein H. americanus brain extracts. AMGSEFLamide, of which there are multiple copies in the cloned transcripts, was the most abundant isoform detected in the brain. Because the GSEFLamides are present in the lobster nervous system, we hypothesized that they might function as neuromodulators, as is common for neuropeptides. We thus asked whether AMGSEFLamide modulates the rhythmic outputs of the cardiac ganglion and the stomatogastric ganglion. Physiological recordings showed that AMGSEFLamide potently modulates the motor patterns produced by both ganglia, suggesting that the GSEFLamides may serve as important and conserved modulators of rhythmic motor activity in arthropods.

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Section: Gseflamides Appear Broadly Conserved Within the Arthropoda contrasting

confidence: 63%

Section: Conservation Of the Gseflamide Family In The Arthropodamentioning

confidence: 99%

AMGSEFLamide, a member of a broadly conserved peptide family, modulates multiple neural networks inHomarus americanus

Dickinson

Oleisky

Rivera

et al. 2018

Journal of Experimental Biology

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“…The structures of mature peptides were predicted using a well-established workflow ( e.g., Christie, 2008a, 2008, 2014a, 2014b, 2014c, 2014d, 2014e, 2014f, 2015a, 2015b, 2015c, 2015d, 2016a, 2016b; Christie and Chi, 2015a, 2015b, 2015c; Christie and Pascual, 2016; Christie et al, 2008b, 2010b, 2011b, 2011c, 2011d, 2013, 2015; Gard et al, 2009; Ma et al, 2009b, 2010). Specifically, each of the deduced precursor proteins was assessed for the presence of a signal peptide using the online program SignalP 4.1 (http://www.cbs.dtu.dk/services/SignalP/; Petersen et al, 2011); the D-cutoff values of SignalP 4.1 were set to “Sensitive” to better match the sensitivity of version 3.0 of this freeware program.…”

Section: Methodsmentioning

confidence: 99%

Prediction of a neuropeptidome for the eyestalk ganglia of the lobster Homarus americanus using a tissue-specific de novo assembled transcriptome

Christie

Roncalli

Cieslak

et al. 2017

General and Comparative Endocrinology

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In silico transcriptome mining is a powerful tool for crustacean peptidome prediction. Using homology-based BLAST searches and a simple bioinformatics workflow, large peptidomes have recently been predicted for a variety of crustaceans, including the lobster, Homarus americanus. Interestingly, no in silico studies have been conducted on the eyestalk ganglia (lamina ganglionaris, medulla externa, medulla interna and medulla terminalis) of the lobster, although the eyestalk is the location of a major neuroendocrine complex, i.e., the X-organ-sinus gland system. Here, an H. americanus eyestalk ganglia-specific transcriptome was produced using the de novo assembler Trinity. This transcriptome was generated from 130,973,220 Illumina reads and consists of 147,542 unique contigs. Eighty-nine neuropeptide-encoding transcripts were identified from this dataset, allowing for the deduction of 62 distinct pre/preprohormones. Two hundred sixty-two neuropeptides were predicted from this set of precursors; the peptides include members of the adipokinetic hormone-corazonin-like peptide, allatostatin A, allatostatin B, allatostatin C, bursicon α, CCHamide, corazonin, crustacean cardioactive peptide, crustacean hyperglycemic hormone (CHH), CHH precursor-related peptide, diuretic hormone 31, diuretic hormone 44, eclosion hormone, elevenin, FMRFamide-like peptide, glycoprotein hormone α2, glycoprotein hormone β5, GSEFLamide, intocin, leucokinin, molt-inhibiting hormone, myosuppressin, neuroparsin, neuropeptide F, orcokinin, orcomyotropin, pigment dispersing hormone, proctolin, pyrokinin, red pigment concentrating hormone, RYamide, short neuropeptide F, SIFamide, sulfakinin, tachykinin-related peptide and trissin families. The predicted peptides expand the H. americanus eyestalk ganglia neuropeptidome approximately 7-fold, and include 78 peptides new to the lobster. The transcriptome and predicted neuropeptidome described here provide new resources for investigating peptidergic signaling within/from the lobster eyestalk ganglia.

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“…This family is defined by a conserved 5-amino acid C-terminal sequence (FXPRL-amide, X = G, T, I, V, K, A, P, S or D), which is the minimal sequence required for physiological activity [ 9 ]. This motif is conserved throughout several arthropod taxa, such as Arachnida [ 10 – 12 ], Protura [ 13 ], Crustacea [ 3 , 14 , 15 ] and Insecta (reviewed in [ 9 ]). Peptides of the PK/PBAN/DH family are myotropic and promote pheromone biosynthesis in different insect orders [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Pyrokinin receptor silencing in females of the southern cattle tick Rhipicephalus (Boophilus) microplus is associated with a reproductive fitness cost

et al. 2022

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Background Rhipicephalusmicroplus is the vector of deadly cattle pathogens, especially Babesia spp., for which a recombinant vaccine is not available. Therefore, disease control depends on tick vector control. However, R.microplus populations worldwide have developed resistance to available acaricides, prompting the search for novel acaricide targets. G protein-coupled receptors (GPCRs) are involved in the regulation of many physiological processes and have been suggested as druggable targets for the control of arthropod vectors. Arthropod-specific signaling systems of small neuropeptides are being investigated for this purpose. The pyrokinin receptor (PKR) is a GPCR previously characterized in ticks. Myotropic activity of pyrokinins in feeding-related tissues of Rhipicephalussanguineus and Ixodesscapularis was recently reported. Methods The R.microplus pyrokinin receptor (Rhimi-PKR) was silenced through RNA interference (RNAi) in female ticks. To optimize RNAi, a dual-luciferase assay was applied to determine the silencing efficiency of two Rhimi-PKR double-stranded RNAs (dsRNA) prior to injecting dsRNA in ticks to be placed on cattle. Phenotypic variables of female ticks obtained at the endpoint of the RNAi experiment were compared to those of control female ticks (non-injected and beta-lactamase dsRNA-injected). Rhimi-PKR silencing was verified by quantitative reverse-transcriptase PCR in whole females and dissected tissues. Results The Rhimi-PKR transcript was expressed in all developmental stages. Rhimi-PKR silencing was confirmed in whole ticks 4 days after injection, and in the tick carcass, ovary and synganglion 6 days after injection. Rhimi-PKR silencing was associated with an increased mortality and decreased weight of both surviving females and egg masses (P < 0.05). Delays in repletion, pre-oviposition and incubation periods were observed (P < 0.05). Conclusions Rhimi-PKR silencing negatively affected female reproductive fitness. The PKR appears to be directly or indirectly associated with the regulation of female feeding and/or reproductive output in R.microplus. Antagonists of the pyrokinin signaling system could be explored for tick control. Graphical abstract

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Identification of the first neuropeptides from the enigmatic hexapod order Protura

Cited by 18 publications

References 60 publications

AMGSEFLamide, a member of a broadly conserved peptide family, modulates multiple neural networks inHomarus americanus

AMGSEFLamide, a member of a broadly conserved peptide family, modulates multiple neural networks inHomarus americanus

Prediction of a neuropeptidome for the eyestalk ganglia of the lobster Homarus americanus using a tissue-specific de novo assembled transcriptome

Pyrokinin receptor silencing in females of the southern cattle tick Rhipicephalus (Boophilus) microplus is associated with a reproductive fitness cost

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