Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

Qi, Yuting; Gu, Caihong; Wang, Xingjun; Gao, Shiqing; Li, Changsheng; Zhao, Chuanzhi; Li, Chuanshun; Ma, Changle; Zhao, Dazun; Zhang, Quan

doi:10.21203/rs.2.18301/v3

Cited by 1 publication

(1 citation statement)

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“…In the red Chinese cabbage, transcriptome analysis showed that BrMYB90 was strongly expressed in the anthocyanin biosynthetic pathway (Rameneni et al, 2020). In Eutrema salsugineum, EsMYB90 was identified as a regulator of anthocyanin biosynthesis and could enhance anthocyanin accumulation in various organs via ectopic expression in tobacco and Arabidopsis (Qi et al, 2020). PalMYB90 is highly expressed in Populus, and was found to regulate key flavonoid pathways to elevate anthocyanin levels in transgenic plants (Bai et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of the R2R3-MYB Gene Family in Fragaria × ananassa and Its Function Identification During Anthocyanins Biosynthesis in Pink-Flowered Strawberry

Liu

Wang

et al. 2021

Front. Plant Sci.

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The strawberry (Fragaria × ananassa) is an economically important fruit throughout the world. The large R2R3-MYB gene family participates in a variety of plant functions, including anthocyanin biosynthesis. The present study is the first genome-wide analysis of the MYB gene family in the octoploid strawberry and describes the identification and characterization of the family members using the recently sequenced F. × ananassa genome. Specifically, we aimed to identify the key MYBs involved in petal coloration in the pink-flowered strawberry, which increases its ornamental value. A comprehensive, genome-wide analysis of F. × ananassa R2R3-FaMYBs was performed, investigating gene structures, phylogenic relationships, promoter regions, chromosomal locations, and collinearity. A total of 393 R2R3-FaMYB genes were identified in the F. × ananassa genome and divided into 36 subgroups based on phylogenetic analysis. Most genes with similar functions in the same subgroup exhibited similar exon-intron structures and motif compositions. These R2R3-FaMYBs were unevenly distributed over 28 chromosomes. The expansion of the R2R3-FaMYB gene family in the F. × ananassa genome was found to be caused mainly by segmental duplication. The Ka/Ks analysis indicated that duplicated R2R3-FaMYBs mostly experienced purifying selection and showed limited functional divergence after the duplication events. To elucidate which R2R3-FaMYB genes were associated with anthocyanin biosynthesis in the petals of the pink-flowered strawberry, we compared transcriptional changes in different flower developmental stages using RNA-seq. There were 131 differentially expressed R2R3-FaMYB genes identified in the petals, of which three genes, FaMYB28, FaMYB54, and FaMYB576, appeared likely, based on the phylogenetic analysis, to regulate anthocyanin biosynthesis. The qRT-PCR showed that these three genes were more highly expressed in petals than in other tissues (fruit, leaf, petiole and stolon) and their expressions were higher in red compared to pink and white petals. These results facilitate the clarification on the roles of the R2R3-FaMYB genes in petal coloration in the pink-flowered strawberry. This work provides useful information for further functional analysis on the R2R3-FaMYB gene family in F. × ananassa.

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Section: Discussionmentioning

confidence: 99%