Identification of the Edible Fig `Bianco del Cilento' by Random Amplified Polymorphic DNA Analysis

Abstract

<jats:p>Random amplified polymorphic DNA (RAPD) analysis is currently used to estimate genetic relationships in plants. We have used RAPD analysis to distinguish six different cultivars of <jats:italic>Ficus carica</jats:italic>, and several of their clones<jats:italic>,</jats:italic> that are widespread in the Campania Region of Southern Italy. Among these cultivars, `Bianco del Cilento' has unique characteristics, and is particularly useful for drying and for the manufacture of syrups. The protection of this cultivar is important to the Campania Region. We have utilized molecular markers to allow accurate identification of this cultivar, making it possible to control the quality of products and prevent fraudulent commerce. DNA was extracted from leaves and amplified by PCR using random oligonucleotide primers. The amplification patterns obtained with five decamer primers were useful for distinguishing all six cultivars analyzed. `Bianco del Cilento' was identified by two primers. The banding patterns were scored and used in similarity value calculations to estimate genetic relationships.</jats:p>

Genotyping of fig (Ficus carica L) via RAPD markers

“…The resulted level of intra-cultivar clonal similarity was very high and homogeneous, in agreement with Galderisi et al 17 In the same way, the 'Columbra Bianca' accessions showed very close similarity coefficients (NLc > 0.9). In particular, NLc = 0.96 for CBF 12 versus CBF 3D clone, NLc = 0.94 for CBF 3D versus CBF 9C and NLc = 0.93 for CBF 9C versus CBF 12.…”

“…Previous works demonstrated that a low number of amplicons per primer was sufficient to produce useful fingerprints for cultivar and clone discrimination. 17,18 In particular, Galderisi et al 17 have reported that only two primers were sufficient to distinguish F carica cultivars. Figure 2 is an example of RAPD fingerprints, obtained using U1 primer on the nine reference cultivars, which demonstrate the cultivar diversity and the intra-variety homogeneity.…”

“…17,20,21 The quality of DNA template is a crucial factor for successful PCR. This is City, CA, USA) and 10 ng of fig genomic DNA.…”

“…16 -19 In related papers the experimental conditions for the cultivar identification of common fig by RAPD-PCR were carried out on a limited number of fig samples. 17,20,21 In this work the CTAB-based DNA isolation protocol was followed by RAPD-PCR screening and applied to a number of important Calabrian fig cultivars which were selected on the basis of their geographical proximity, morphological similarity and industrial potential for drying.…”

Analysis of diversity in the fruit tree genetic resources from a Mediterranean island

“…These genetic resources showed well differentiated phenotypes, while in some species such as fig the genetic variability was lowered by the high level of homozygosis. Similar results on local fig genetic resources were obtained in other areas of southern Italy (Galderisi et al 1999), as well as in four different areas in France (Khadari et al 1995).…”