Identification of the differentially accumulated proteins associated with low phosphorus tolerance in a Tibetan wild barley accession

Nadira, Umme Aktari; Ahmed, Imrul Mosaddek; Zeng, Jianbin; Wu, Feibo; Zhang, Guoping

doi:10.1016/j.jplph.2016.03.016

Cited by 11 publications

(10 citation statements)

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“…According to Nadira et al [ 37 ], total protein was extracted by the phenol extraction method. The leaf samples were ground to a fine powder form in liquid nitrogen using a mortar and pestle.…”

Section: Methodsmentioning

confidence: 99%

“…Extracted proteins were separated using two-dimensional gel electrophoresis (2-DE) with isoelectric focusing (IEF) as the first dimension [ 37 ]. Protein visualization, image analysis, and quantification were carried out as described previously by Nadira et al [ 37 ]. All the spots exhibiting differential accumulation with more than 1.5 fold between treatments and control were selected for statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

“…MALDI-TOF/TOF MS analysis and protein identification of the tryptic-digested peptide mass fingerprint were evaluated using a MALDI-TOF/TOF mass spectrometer (ABI4700 System, CA, USA) according to Nadira et al [ 37 ]. Target peptides with a MASCOT (Mascot is a software search engine) score more than 72 or a best ion score, based on both MS and MS/MS spectra, were considered to be significant ( p < 0.05) candidate proteins.…”

Section: Methodsmentioning

confidence: 99%

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The Barley S-Adenosylmethionine Synthetase 3 Gene HvSAMS3 Positively Regulates the Tolerance to Combined Drought and Salinity Stress in Tibetan Wild Barley

Ahmed

Nadira

Qiu

et al. 2020

Cells

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Drought and salinity are two of the most frequently co-occurring abiotic stresses. Despite recent advances in the elucidation of the effects of these stresses individually during the vegetative stage of plants, significant gaps exist in our understanding of the combined effects of these two frequently co-occurring stresses. Here, Tibetan wild barley XZ5 (drought tolerant), XZ16 (salt tolerant), and cultivated barley cv. CM72 (salt tolerant) were subjected to drought (D), salinity (S), or a combination of both treatments (D+S). Protein synthesis is one of the primary activities of the green part of the plant. Therefore, leaf tissue is an important parameter to evaluate drought and salinity stress conditions. Sixty differentially expressed proteins were identified by mass spectrometry (MALDI-TOF/TOF) and classified into 9 biological processes based on Gene Ontology annotation. Among them, 21 proteins were found to be expressed under drought or salinity alone; however, under D+S, 7 proteins, including S-adenosylmethionine synthetase 3 (SAMS3), were exclusively upregulated in drought-tolerant XZ5 but not in CM72. HvSAMS3 carries both N-terminal and central domains compared with Arabidopsis and activates the expression of several ethylene (ET)-responsive transcription factors. HvSAMS3 is mainly expressed in the roots and stems, and HvSAMS3 is a secretory protein located in the cell membrane and cytoplasm. Barley stripe mosaic virus-based virus-induced gene silencing (BSMV-VIGS) of HvSAMS3 in XZ5 severely compromised its tolerance to D+S and significantly reduced plant growth and K+ uptake. The reduced tolerance to the combined stress was associated with the inhibition of polyamines such as spermidine and spermine, polyamine oxidase, ethylene, biotin, and antioxidant enzyme activities. Furthermore, the exogenous application of ethylene and biotin improved the tolerance to D+S in BSMV-VIGS:HvSAMS3-inoculated plants. Our findings highlight the significance of HvSAMS3 in the tolerance to D+S in XZ5.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Barley S-Adenosylmethionine Synthetase 3 Gene HvSAMS3 Positively Regulates the Tolerance to Combined Drought and Salinity Stress in Tibetan Wild Barley

Ahmed

Nadira

Qiu

et al. 2020

Cells

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“…Transcriptomic, proteomic, and metabolomic platforms have been applied to characterize the Pi stress response (Sha et al, 2016;Luo et al, 2018;Ren et al, 2018). A proteomic study showed that many differentially accumulated proteins took part in carbon and energy metabolism, signal transduction, secondary metabolism, and stress defense associated with low-Pi tolerance in barley (Nadira et al, 2016). Recently, posttranscriptional modification was shown to play a key role in the plant response to P deficiency (Park et al, 2014;Yang et al, 2019Yang et al, , 2020Wang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Global Profiling of Phosphorylation Reveals the Barley Roots Response to Phosphorus Starvation and Resupply

Wang

et al. 2021

Front. Plant Sci.

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Phosphorus (P) deficiency is a major threat to the crop production, and for understanding the response mechanism of plant roots, P stress may facilitate the development of crops with increased tolerance. Phosphorylation plays a critical role in the regulation of proteins for plant responses to biotic and abiotic stress; however, its functions in P starvation/resupply are largely unknown for barley (Hordeum vulgare) growth. Here, we performed a global review of phosphorylation in barley roots treated by P starvation/resupply. We identified 7,710 phosphorylation sites on 3,373 proteins, of which 76 types of conserved motifs were extracted from 10,428 phosphorylated peptides. Most phosphorylated proteins were located in the nucleus (36%) and chloroplast (32%). Compared with the control, 186 and 131 phosphorylated proteins under P starvation condition and 156 and 111 phosphorylated proteins under P resupply condition showed significant differences at 6 and 48 h, respectively. These proteins mainly participated in carbohydrate metabolism, phytohormones, signal transduction, cell wall stress, and oxidases stress. Moreover, the pathways of the ribosome, RNA binding, protein transport, and metal binding were significantly enriched under P starvation, and only two pathways of ribosome and RNA binding were greatly enriched under Pi resupply according to the protein–protein interaction analysis. The results suggested that the phosphorylation proteins might play important roles in the metabolic processes of barley roots in response to Pi deficiency/resupply. The data not only provide unique access to phosphorylation reprogramming of plant roots under deficiency/resupply but also demonstrate the close cooperation between these phosphorylation proteins and key metabolic functions.

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“…The wild barley accessions have been reported with outstanding abilities of tolerance to various abiotic stresses, including drought, salinity, aluminum toxicity and nitrogen deficiency [34–37]. Low-P tolerant accessions were identified in the previous study [38], however, the molecular mechanisms and the relevant genes are not clarified.…”

Section: Introductionmentioning

confidence: 99%

Root plasticity and Pi recycling within plants contribute to low-P tolerance in Tibetan wild barley

Long

et al. 2019

BMC Plant Biol

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Background Barley is a low phosphorus (P) demand cereal crop. Tibetan wild barley, as a progenitor of cultivated barley, has revealed outstanding ability of tolerance to low-P stress. However, the underlying mechanisms of low-P adaption and the relevant genetic controlling are still unclear. Results We identified low-P tolerant barley lines in a doubled-haploid (DH) population derived from an elite Tibetan wild barley accession and a high-yield cultivar. The tolerant lines revealed greater root plasticity in the terms of lateral root length, compared to low-P sensitive lines, in response to low-P stress. By integrating the QTLs associated with root length and root transcriptomic profiling, candidate genes encoding isoflavone reductase, nitrate reductase, nitrate transporter and transcriptional factor MYB were identified. The differentially expressed genes (DEGs) involved the growth of lateral root, Pi transport within cells as well as from roots to shoots contributed to the differences between low-P tolerant line L138 and low-P sensitive lines L73 in their ability of P acquisition and utilization. Conclusions The plasticity of root system is an important trait for barley to tolerate low-P stress. The low-P tolerance in the elite DH line derived from a cross of Tibetan wild barley and cultivated barley is characterized by enhanced growth of lateral root and Pi recycling within plants under low-P stress. Electronic supplementary material The online version of this article (10.1186/s12870-019-1949-x) contains supplementary material, which is available to authorized users.

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Identification of the differentially accumulated proteins associated with low phosphorus tolerance in a Tibetan wild barley accession

Cited by 11 publications

References 42 publications

The Barley S-Adenosylmethionine Synthetase 3 Gene HvSAMS3 Positively Regulates the Tolerance to Combined Drought and Salinity Stress in Tibetan Wild Barley

The Barley S-Adenosylmethionine Synthetase 3 Gene HvSAMS3 Positively Regulates the Tolerance to Combined Drought and Salinity Stress in Tibetan Wild Barley

Global Profiling of Phosphorylation Reveals the Barley Roots Response to Phosphorus Starvation and Resupply

Root plasticity and Pi recycling within plants contribute to low-P tolerance in Tibetan wild barley

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