Identification of the different salivary Interleukin-6 profiles in patients with periodontitis: A cross-sectional study

Isola, Gaetano; Giudice, Antonino Lo; Alibrandi, Angela; Murabito, Paolo; Indelicato, Francesco

doi:10.1016/j.archoralbio.2020.104997

Cited by 78 publications

(84 citation statements)

References 53 publications

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“…Tobacco and alcohol seem to be the major risk factors for OSCC, but recent evidence also shows an association of other factors, such as inflammation and oral microbiome, in oral cancer. There seem to be several inflammatory mediators, salivary proteins, and oral microbiota that are common between inflammatory diseases of the oral cavity, such as periodontitis and oral cancer [ 2 , 3 , 4 ]. Despite some advances in diagnosis and treatment, the 5-year survival rate for OSCC is less than 50% across all stages, and has not greatly improved for the past few decades [ 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Cell Culture Models to Investigate Oral Carcinogenesis: A Scoping Review

Suryaprakash

Kujan

Shearston

et al. 2020

IJMS

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Three-dimensional (3-D) cell culture models, such as spheroids, organoids, and organotypic cultures, are more physiologically representative of the human tumor microenvironment (TME) than traditional two-dimensional (2-D) cell culture models. They have been used as in vitro models to investigate various aspects of oral cancer but, to date, have not be widely used in investigations of the process of oral carcinogenesis. The aim of this scoping review was to evaluate the use of 3-D cell cultures in oral squamous cell carcinoma (OSCC) research, with a particular emphasis on oral carcinogenesis studies. Databases (PubMed, Scopus, and Web of Science) were systematically searched to identify research applying 3-D cell culture techniques to cells from normal, dysplastic, and malignant oral mucosae. A total of 119 studies were included for qualitative analysis including 53 studies utilizing spheroids, 62 utilizing organotypic cultures, and 4 using organoids. We found that 3-D oral carcinogenesis studies had been limited to just two organotypic culture models and that to date, spheroids and organoids had not been utilized for this purpose. Spheroid culture was most frequently used as a tumorosphere forming assay and the organoids cultured from human OSCCs most often used in drug sensitivity testing. These results indicate that there are significant opportunities to utilize 3-D cell culture to explore the development of oral cancer, particularly as the physiological relevance of these models continues to improve.

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Section: Introductionmentioning

confidence: 99%

Three-Dimensional Cell Culture Models to Investigate Oral Carcinogenesis: A Scoping Review

Suryaprakash

Kujan

Shearston

et al. 2020

IJMS

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“…With the advantage of fast and easy to conduct, various methods were developed for early diagnosis of diseases, including oral diseases, neurodegenerative diseases, systemic infection, by evaluating the salivary biomarkers [ 24 ]. More specifically, periodontitis, the most prevalent oral disease, can be detected early by monitoring the level of soluble urokinase-type plasminogen activator receptor (suPAR), or Interleukin-6 (IL-6), or bacterial specific antibodies in saliva [ 25 , 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- Mice

Toan

Tai

Kim

et al. 2021

IJMS

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Salivary gland dysfunction induces salivary flow reduction and a dry mouth, and commonly involves oral dysfunction, tooth structure deterioration, and infection through reduced salivation. This study aimed to investigate the impact of aging on the salivary gland by a metabolomics approach in an extensive aging mouse model, SAMP1/Klotho -/- mice. We found that the salivary secretion of SAMP1/Klotho -/- mice was dramatically decreased compared with that of SAMP1/Klotho WT (+/+) mice. Metabolomics profiling analysis showed that the level of acetylcholine was significantly decreased in SAMP1/Klotho -/- mice, although the corresponding levels of acetylcholine precursors, acetyl-CoA and choline, increased. Interestingly, the mRNA and protein expression of choline acetyltransferase (ChAT), which is responsible for catalyzing acetylcholine synthesis, was significantly decreased in SAMP1/Klotho -/- mice. The overexpression of ChAT induced the expression of salivary gland functional markers (α–amylase, ZO-1, and Aqua5) in primary cultured salivary gland cells from SAMP1/Klotho +/+ and -/- mice. In an in vivo study, adeno-associated virus (AAV)-ChAT transduction significantly increased saliva secretion compared with the control in SAMP1/Klotho -/- mice. These results suggest that the dysfunction in acetylcholine biosynthesis induced by ChAT reduction may cause impaired salivary gland function

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“…An association between Ob and periodontitis was previously reported [ 8 – 10 ]. Variation in the production of local and systemic inflammatory mediators was related to BMI and periodontal disease [ 11 – 13 ]. Thus far, the mechanism explaining the relationship between Owt or Ob and periodontitis is not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Periodontal Treatment Improves Serum Levels of Leptin, Adiponectin, and C-Reactive Protein in Thai Patients with Overweight or Obesity

Wanichkittikul

Laohapand

Mansanguan

et al. 2021

International Journal of Dentistry

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Periodontitis and overweight or obesity independently change serum levels of leptin, adiponectin, and C-reactive protein (CRP). The aim of this study is to investigate the alterations of serum levels of leptin, adiponectin, and CRP after nonsurgical periodontal treatment (NSPT) in Thai patients with overweight or obesity (Owt/Ob) who did or did not exhibit severe periodontitis (SP) and normal weight (Nwt) patients with or without SP. Two hundred sixty patients were screened; 29 patients were included in this study. The study participants comprised 6 patients with Owt/Ob who exhibited SP, 11 patients with Owt/Ob who did not exhibit SP, 5 Nwt patients with SP, and 7 Nwt patients without SP. Periodontal disease status was evaluated; serum levels of leptin, adiponectin, and CRP were determined by enzyme-linked immunosorbent assay at baseline, as well as at 3 and 6 months after NSPT. At 3 months after NSPT, periodontal status was improved in all groups ( p < 0.05), except Nwt patients without SP. Serum levels of leptin and CRP were significantly reduced, while serum levels of adiponectin were elevated after NSPT, regardless of bodyweight or waist circumference ( p < 0.05). Improvement in serum levels of leptin after NSPT was also observed in the Nwt with SP group ( p = 0.015); these levels did not significantly differ in Nwt patients without SP. NSPT reduces serum levels of leptin and CRP and enhances serum levels of adiponectin in Thai patients with Owt/Ob, irrespective of periodontitis severity. These results suggest a role for periodontal treatment in the systemic inflammatory response of Thai people with Owt/Ob.

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Identification of the different salivary Interleukin-6 profiles in patients with periodontitis: A cross-sectional study

Cited by 78 publications

References 53 publications

Three-Dimensional Cell Culture Models to Investigate Oral Carcinogenesis: A Scoping Review

Three-Dimensional Cell Culture Models to Investigate Oral Carcinogenesis: A Scoping Review

Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- Mice

Periodontal Treatment Improves Serum Levels of Leptin, Adiponectin, and C-Reactive Protein in Thai Patients with Overweight or Obesity

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