Identification of the cytochrome P450 involved in the degradation of neonicotinoid insecticide acetamiprid in Phanerochaete chrysosporium

Wang, Jianqiao; Ohno, Haruka; Ide, Yuuri; Ichinose, Hirofumi; Mori, Toshio; Kawagishi, Hirokazu; Hirai, Hirofumi

doi:10.1016/j.jhazmat.2019.03.042

Cited by 55 publications

(18 citation statements)

References 46 publications

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“…Therefore, some studies attempted to express and functionally analyse the entire CYPome of P. chrysosporium and model brown-rot fungus Postia placenta (Hirosue et al 2011;Ide et al 2012;Ichinose 2013). A few individual P450s with interesting activities were also looked at more closely (Kasai et al 2009;Syed et al 2010;Kasai et al 2010a;Kasai et al 2010b;Chigu et al 2010;Syed et al 2011;Ichinose and Wariishi 2012;Syed et al 2013b;Syed et al 2013c;Hatakeyama et al 2016;Sakai et al 2018;Yang et al 2018;Wang et al 2019). However, the research about fungal P450s is still in its early stages and often shares ideas and limited information rather than comprehensive details on the P450 function.…”

Section: Introductionmentioning

confidence: 99%

Evolution and enrichment of CYP5035 in Polyporales: functionality of an understudied P450 family

Fessner

Nelson

Glieder

2021

Appl Microbiol Biotechnol

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Bioprospecting for innovative basidiomycete cytochrome P450 enzymes (P450s) is highly desirable due to the fungi’s enormous enzymatic repertoire and outstanding ability to degrade lignin and detoxify various xenobiotics. While fungal metagenomics is progressing rapidly, the biocatalytic potential of the majority of these annotated P450 sequences usually remains concealed, although functional profiling identified several P450 families with versatile substrate scopes towards various natural products. Functional knowledge about the CYP5035 family, for example, is largely insufficient. In this study, the families of the putative P450 sequences of the four white-rot fungi Polyporus arcularius, Polyporus brumalis, Polyporus squamosus and Lentinus tigrinus were assigned, and the CYPomes revealed an unusual enrichment of CYP5035, CYP5136 and CYP5150. By computational analysis of the phylogeny of the former two P450 families, the evolution of their enrichment could be traced back to the Ganoderma macrofungus, indicating their evolutionary benefit. In order to address the knowledge gap on CYP5035 functionality, a representative subgroup of this P450 family of P. arcularius was expressed and screened against a test set of substrates. Thereby, the multifunctional enzyme CYP5035S7 converting several plant natural product classes was discovered. Aligning CYP5035S7 to 102,000 putative P450 sequences of 36 fungal species from Joint Genome Institute-provided genomes located hundreds of further CYP5035 family members, which subfamilies were classified if possible. Exemplified by these specific enzyme analyses, this study gives valuable hints for future bioprospecting of such xenobiotic-detoxifying P450s and for the identification of their biocatalytic potential. Graphical abstract Key points • The P450 families CYP5035 and CYP5136 are unusually enriched in P. arcularius. • Functional screening shows CYP5035 assisting in the fungal detoxification mechanism. • Some Polyporales encompass an unusually large repertoire of detoxification P450s.

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Section: Introductionmentioning

confidence: 99%

Evolution and enrichment of CYP5035 in Polyporales: functionality of an understudied P450 family

Fessner

Nelson

Glieder

2021

Appl Microbiol Biotechnol

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“…A series paper has to describe that Phanerochaete sp. P450 plays a critical role in the degradation of a neonicotinoid insecticide [49][50][51][52]. Acetamiprid (ACET) has been widely used for the last two decades, persistence in environments, potential risks to non targets, and one of the first-generation neonicotinoid insecticides.…”

Section: Fungal P450 As a Biodegrader Of Pesticidesmentioning

confidence: 99%

Bio function of Cytochrome P450 on fungus: a review

Pratiwi

Yahya

Chi

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Cytochrome P450 is the superfamily of proteins involved in the metabolism of organisms, including fungi. Fungal have more diverse P450 families than plants, animals, or bacteria. Research on fungal P450 has blossomed and become an important area in biology and ecology. Cytochrome P450 could be detoxifying natural and environmental contaminants to survive in several ecological niches. Furthermore, the presence of the fungal Cytochrome P450 as an antifungal drug target is a promising approach for the controlling of pest and plant pathogenic fungi. To date, numerous studies have revealed the annotation of diverse P450 followed by an elucidation of P450 functions. This mini-review starts with some basic information of P450s on fungi, then discusses the incredible bio function of characterized fungal P450.

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“…Cytochrome P450s played a vital role in the metabolism of organic pollutants using rot fungi ( Mori et al, 2017 ). Wang et al (2019a) demonstrated the enzyme P450 (enriched by Phanerochaete chrysosporium ) to degrade neonicotinoid insecticide acetamiprid effectively. After the incubation of 20 days, P. chrysosporium eliminate 21% and 51% of acetamiprid in two different media such as ligninolytic and non-ligninolytic, respectively.…”

Section: Microbial Technologies For the Enhanced Neonicotinoid Biodegradationmentioning

confidence: 99%

Microbial Technologies Employed for Biodegradation of Neonicotinoids in the Agroecosystem

et al. 2021

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Neonicotinoids are synthetic pesticides widely used for the control of various pests in agriculture throughout the world. They mainly attack the nicotinic acetylcholine receptors, generate nervous stimulation, receptor clot, paralysis and finally cause death. They are low volatile, highly soluble and have a long half-life in soil and water. Due to their extensive use, the environmental residues have immensely increased in the last two decades and caused many hazardous effects on non-target organisms, including humans. Hence, for the protection of the environment and diversity of living organism’s the degradation of neonicotinoids has received widespread attention. Compared to the other methods, biological methods are considered cost-effective, eco-friendly and most efficient. In particular, the use of microbial species makes the degradation of xenobiotics more accessible fast and active due to their smaller size. Since this degradation also converts xenobiotics into less toxic substances, the various metabolic pathways for the microbial degradation of neonicotinoids have been systematically discussed. Additionally, different enzymes, genes, plasmids and proteins are also investigated here. At last, this review highlights the implementation of innovative tools, databases, multi-omics strategies and immobilization techniques of microbial cells to detect and degrade neonicotinoids in the environment.

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Identification of the cytochrome P450 involved in the degradation of neonicotinoid insecticide acetamiprid in Phanerochaete chrysosporium

Cited by 55 publications

References 46 publications

Evolution and enrichment of CYP5035 in Polyporales: functionality of an understudied P450 family

Evolution and enrichment of CYP5035 in Polyporales: functionality of an understudied P450 family

Bio function of Cytochrome P450 on fungus: a review

Microbial Technologies Employed for Biodegradation of Neonicotinoids in the Agroecosystem

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