Identification of the conserved structural elements in the ribosomal protein L10 which are essential for the autogenous regulation of gene expression in the rplJL operon of Enterobacteria

Abstract

Проведен поиск элементов структурной организации белка ЫО, которые необходимы для взаимодействия этого белка с мРНКовыми мишенями, лежащего в основе выпол нения белком ЫО функции аутогенной регуляции экспрессии генов rplJL энтеробактерий. Во взаимодействии с мРНКовыми мишенями у энтеробактерий могут участвовать следующие структурные элементы ЫО: мотив-kNTll-, мотив-Eif-и структура альфа-бета-альфа (98-111).

The potential mRNA target sites for the ribosomal protein L10 found in Streptomyces griseus and S. coelicolor give evidence for the autogenous regulation of expression of the rplJL genes

“…The identical elements of the mRNA and rRNA targets (ssA region and the C-G pair of dsA) suggest a complementary structural conservation in the regulatory protein. We reason that the; -RNTLL-motif, which is the only one conserved among the 17 analyzed L10 protein sequences, is complementary to the conserved structural ele ments in dsA and ssA regions of the RNA target sites [9].…”

“…This makes it reasonable to as sume that the L10-RNA interaction, which mediates the autogenous re gulation of the rplJL genes expression is species-and organism-specific. Existence of the «specifying» complementary structural elements, is to be found in both, the regulatory proteins [9] and their mRNA binding sites [6][7][8]. The latter might be of importance to provide the efficient recognition and tight binding the LIO protein to the mRNA, which in the case of the rRNA target would be achieved via the cooperative binding of the neighbouring r-proteins.…”