Identification of the Combinatorial Effect of miRNA Family Regulatory Network in Different Growth Patterns of GC

Molecular Therapy - Nucleic Acids

et al. 2021

Hepatocellular carcinoma (HCC) remains an extremely lethal disease worldwide. High-throughput methods have revealed global transcriptome dysregulation; however, a comprehensive investigation of the complexity and behavioral characteristics of the competing endogenous RNA (ceRNA) network in HCC is lacking. In this study, we extracted the transcriptome (RNA) sequencing data of 371 HCC patients from The Cancer Genome Atlas platform. With the comparison of the high Myc expression (Myc high ) tumor and low Myc expression (Myc low ) tumor groups in HCC, we identified 1,125 differentially expressed (DE) mRNAs, 589 long non-coding RNAs (lncRNAs), and 93 microRNAs (miRNAs). DE RNAs predicted the interactions necessary to construct an associated Myc ceRNA network, including 19 DE lncRNAs, 5 miRNAs, and 72 mRNAs. We identified a significant signature (long intergenic non-protein-coding [LINC] RNA 2691 [LINC02691] and LINC02499) that effectively predicted overall survival and had protective effects. The target genes of microRNA (miR)-212-3p predicted to intersect with DE mRNAs included SEC14-like protein 2 (SEC14L2) and solute carrier family 6 member 1 (SLC6A1), which were strongly correlated with survival and prognosis. With the use of the lncRNA-miRNA-mRNA axis, we constructed a ceRNA network containing four lncRNAs (LINC02691, LINC02499, LINC01354, and NAV2 antisense RNA 4), one miRNA (miR-212-3p), and two mRNAs (SEC14L2 and SLC6A1). Overall, we successfully constructed a mutually regulated ceRNA network and identified potential precision-targeted therapies and prognostic biomarkers.

Section: Discussionmentioning

confidence: 99%

Construction of a Myc-associated ceRNA network reveals a prognostic signature in hepatocellular carcinoma

Zhang

Shi

Molecular Therapy - Nucleic Acids

et al. 2021

“… 18 Moreover, miRNA-mediated post-transcriptional regulation requires multiple RNA-binding proteins, which are conducive to the function of miRNA in tumorigenesis. 19 , 20 , 21 In the cytoplasm, Salmena et al. 22 proposed the competitive endogenous RNA (ceRNA) hypothesis that lncRNA is described as mainly regulating mRNA through a ceRNA regulation mechanism.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis to identify DLEU2L/TAOK1 axis as a prognostic biomarker in hepatocellular carcinoma

Shi

Zhang

Molecular Therapy - Nucleic Acids

et al. 2021

Hepatocellular carcinoma (HCC) is one of the deadliest malignant tumors that are harmful to human health. Increasing evidence has underscored the critical role of the competitive endogenous RNA (ceRNA) regulatory networks among various human cancers. However, the complexity and behavior characteristics of the ceRNA network in HCC were still unclear. In this study, we aimed to clarify a phosphatase and tensin homolog (PTEN)-related ceRNA regulatory network and identify potential prognostic markers associated with HCC. The expression profiles of three RNAs (long non-coding RNAs [lncRNAs], microRNAs [miRNAs], and mRNAs) were extracted from The Cancer Genome Atlas (TCGA) database. The DLEU2L-hsa-miR-100-5p/ hsa-miR-99a-5p-TAOK1 ceRNA network related to the prognosis of HCC was obtained by performing bioinformatics analysis. Importantly, we identified the DLEU2L/TAOK1 axis in the ceRNA by using correlation analysis, and it appeared to become a clinical prognostic model by Cox regression analysis. Furthermore, methylation analyses suggested that the abnormal upregulation of the DLEU2L/TAOK1 axis likely resulted from hypomethylation, and immune infiltration analysis showed that the DLEU2L/TAOK1 axis may have an impact on the changes in the tumor immune microenvironment and the development of HCC. In summary, the current study constructing a ceRNA-based DLEU2L/TAOK1 axis might be a novel important prognostic factor associated with the diagnosis and prognosis of HCC.

“…microRNA (miRNA) is an endogenous non‐coding RNA with a length of about 22 nucleotides 5,6 . It can inhibit protein translation by binding with the mRNA of coding gene.…”

Section: Introductionmentioning

confidence: 99%

“…microRNA (miRNA) is an endogenous non‐coding RNA with a length of about 22 nucleotides. 5 , 6 It can inhibit protein translation by binding with the mRNA of coding gene. At present, it has been found that there are many miRNAs with abnormal expression in OA patients, which can up‐regulate or down‐regulate the gene expression related to the growth of OA chondrocytes, thus interfering with the occurrence and development of OA.…”

Section: Introductionmentioning

confidence: 99%

The role of circRNA derived from RUNX2 in the serum of osteoarthritis and its clinical value

Wang

Clinical Laboratory Analysis

et al. 2021

Background Circular RNA (circRNA) has been shown to affect the pathological process of osteoarthritis (OA) and is expected to become a potential marker for disease diagnosis. This study aimed to investigate the association between circRNA derived from the gene of runt‐related transcription factor 2 (RUNX2) and OA risk. Methods The expression profile of RUNX2‐derived circRNAs in serum of OA patients was detected. Then, the cytological localization of screened differential circRNAs was studied. Luciferase (LUC) reporter assay was used to identify the microRNA (miRNA) sponge capacity of the circRNAs. Bioinformatics analysis was used to construct the functional pathway of this circRNA‐miRNAs network. And then, the diagnostic value of RUNX2‐derived circRNAs in OA was evaluated. Results RUNX2‐derived hsa_circ_0005526 (circ_RUNX2) is significantly highly expressed in OA serum and mainly located in the cytoplasm within the cartilage cell by sponging multiple miRNAs (miR‐498, miR‐924, miR‐361‐3p, and miR‐665). Bioinformatics analysis showed ECM‐receptor interaction pathway ranked the most significant pathway of circ_RUNX2‐miRNAs regulatory network in KEGG database. The ROC curve showed that there may be good diagnostic value of serum circ_RUNX2 in OA. Conclusion RUNX2‐derived circ_RUNX2 may be involved in OA development via ECM‐receptor interaction pathways and may be used as potential clinical indicator of OA.