Identification of the Coat Protein Gene of a Sweet Potato Sunken Vein Closterovirus Isolate from Kenya and Evidence for a Serological Relationship Among Geographically Diverse Closterovirus Isolates from Sweet Potato

Hoyer, U.; Maiß, E.; Wilhelm, Jochen; Lesemann, D.-E.; Vetten, H. J.

doi:10.1094/phyto-86-744

Cited by 58 publications

(44 citation statements)

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“…However, there are diVerent strains of SPFMV (Cali and Moyer 1981; Kreuze et al 2000) and diVerent serotypes of SPCSV (Alicai et al 1999;Hoyer et al 1996;Vetten et al 1996) in diVerent regions of the world. The diVerent strains and serotypes have biological signiWcance in as much as resistance to SPVD is concerned.…”

Section: Discussionmentioning

confidence: 99%

Identification of molecular markers associated with sweet potato resistance to sweet potato virus disease in Kenya

2007

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Sweet potato virus disease (SPVD), a result of the co-infection of whiteXy transmitted Sweet potato chlorotic stunt virus (genus Crinivirus, family Closteroviridae) and the aphid transmitted Sweet potato feathery mottle virus (genus Potyvirus, family Potyviridae), is the most destructive disease of sweet potato in East Africa. A study was conducted to establish if genotypes identiWed as resistant or susceptible to SPVD in Kenya could be distinguished using molecular markers. A total of 47 unrelated sweet potato genotypes were selected from germplasm collections and classiWed into two phenotypic groups as resistant or susceptible to SPVD. Genotype selection was based on disease severity or days to symptom development in plants following graft inoculation.AmpliWed fragment length polymorphism (AFLP) marker proWles were generated for each individual and used in association studies to identify markers suitable for classifying the two pre-deWned phenotypic groups. Analysis of molecular variance showed signiWcant (P < 0.002) variation between the two groups using 206 polymorphic AFLP markers. Discriminant analysis and logistic regression statistical methods were used to select informative markers, and to develop models that would classify the two phenotypic groups. A training set of 30 genotypes consisting of 15 resistant and 15 susceptible were used to develop classiWcation models. The remaining 17 genotypes were used as a test set. Four markers, which gave 100% correct classiWcation of the training set and 94% correct classiWcation of the test set, were selected by both statistical methods.

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Section: Discussionmentioning

confidence: 99%

Identification of molecular markers associated with sweet potato resistance to sweet potato virus disease in Kenya

2007

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“…The genomic sequence of SPCSV is unknown except for partial sequences of Hsp70h and CP gene sequences from a few isolates (4,28,61).…”

mentioning

confidence: 99%

Complete Genome Sequence and Analyses of the Subgenomic RNAs of Sweet Potato Chlorotic Stunt Virus Reveal Several New Features for the Genus Crinivirus

Kreuze

Savenkov

Valkonen

2002

J Virol

100

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The complete nucleotide sequences of genomic RNA1 (9,407 nucleotides [nt]) and RNA2 (8,223 nt) of Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) were determined, revealing that SPCSV possesses the second largest identified positive-strand single-stranded RNA genome among plant viruses after Citrus tristeza virus. RNA1 contains two overlapping open reading frames (ORFs) that encode the replication module, consisting of the putative papain-like cysteine proteinase, methyltransferase, helicase, and polymerase domains. RNA2 contains the Closteroviridae hallmark gene array represented by a heat shock protein homologue (Hsp70h), a protein of 50 to 60 kDa depending on the virus, the major coat protein, and a divergent copy of the coat protein. This grouping resembles the genome organization of Lettuce infectious yellows virus (LIYV), the only other crinivirus for which the whole genomic sequence is available. However, in striking contrast to LIYV, the two genomic RNAs of SPCSV contained nearly identical 208-nt-long 3 terminal sequences, and the ORF for a putative small hydrophobic protein present in LIYV RNA2 was found at a novel position in SPCSV RNA1. Furthermore, unlike any other plant or animal virus, SPCSV carried an ORF for a putative RNase III-like protein (ORF2 on RNA1). Several subgenomic RNAs (sgRNAs) were detected in SPCSV-infected plants, indicating that the sgRNAs formed from RNA1 accumulated earlier in infection than those of RNA2. The 5 ends of seven sgRNAs were cloned and sequenced by an approach that provided compelling evidence that the sgRNAs are capped in infected plants, a novel finding for members of the Closteroviridae.

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“…After heat denaturation, purified viral dsRNA has been used extensively as the template for reverse transcription PCR (RT-PCR) in plant virus testing (Rott and Jelkmann, 2001;Sabanadzovic and Valverde, 2011;Valverde and Sabanadzovic, 2009;Valverde et al, 2011;Tzanetakis et al 2005). Winter et al (1992) and Hoyer et al (1996) characterized Sweet potato chlorotic stunt virus using dsRNA as reagent for RT-PCR, cloning, and labeling. Zhang and Rowhani (2000) developed a rapid cDNA cloning method from dsRNA templates to partially characterized grape viruses.…”

Section: Reverse Transcription Pcr Cloning and Sequencingmentioning

confidence: 99%

“…Después de someterlo a desnaturalización por calor, el dsRNA viral purificado se ha utilizado extensamente como molde para la transcripción reversa de PCR (RT-PCR) en pruebas con fitovirus (Rott and Jelkmann, 2001;Sabanadzovic y Valverde, 2011;Valverde y Sabanadzovic, 2009;Valverde et al, 2011;Tzanetakis et al 2005). Winter et al (1992) y Hoyer et al (1996) caracterizaron el Virus del enanismo del camote utilizando un reactivo para la RT-PCR, la clonación y el etiquetado. Zhang y Rowhani (2000) desarrollaron un método rápido de clonación de cDNA a partir de moldes de dsRNA para caracterizar parcialmente virus de uva.…”

Section: Reverse Transcription Pcr Cloning and Sequencingunclassified

Extracción y purificación de dsRNAs largos de plantas infectadas con virus y hongos; aplicación en la detección e identificación de virus

Valverde

Torre-Almaráz²

2017

RMF

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Abstract. Various Resumen. Se utilizan varios métodos para realizar la extracción y purificación de ARN de doble cadena (dsRNA) largo de plantas y hongos. Los protocolos más utilizados consisten en la extracción de fenol y la unión selectiva de dsRNA a la celulosa. Los dsRNAs virales purificados de plantas y hongos se han analizado por electroforesis con gel y se han utilizado como herramienta complementaria para identificar virus. Asimismo, los dsRNAs se han utilizado como reactivos en PCR de transcriptasa reversa, clonación molecular, preparación de sondas y secuenciación de virus ARN. Se han descubierto muchos virus ARN y moléculas subvirales que infectan plantas y hongos utilizando protocolos de extracción de dsRNA. Las recientes mejoras a los métodos de extracción de dsRNA han aumentado la eficiencia y la rentabilidad. Se ha comprobado que el dsRNA viral se puede utilizar como reactivo inicial para la secuenciación de próxima generación de genomas virales.

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Identification of the Coat Protein Gene of a Sweet Potato Sunken Vein Closterovirus Isolate from Kenya and Evidence for a Serological Relationship Among Geographically Diverse Closterovirus Isolates from Sweet Potato

Cited by 58 publications

References 0 publications

Identification of molecular markers associated with sweet potato resistance to sweet potato virus disease in Kenya

Identification of molecular markers associated with sweet potato resistance to sweet potato virus disease in Kenya

Complete Genome Sequence and Analyses of the Subgenomic RNAs of Sweet Potato Chlorotic Stunt Virus Reveal Several New Features for the Genus Crinivirus

Extracción y purificación de dsRNAs largos de plantas infectadas con virus y hongos; aplicación en la detección e identificación de virus

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