Identification of the cause of a brain abscess by direct 16S ribosomal DNA sequencing

Logan, J. M. J.; Orange, G.; Maggs, Anthony F.

doi:10.1016/s0163-4453(99)90030-0

Cited by 7 publications

(6 citation statements)

References 9 publications

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“…Se ha utilizado la misma metodología para la identificación directa de agentes infecciosos, a partir de muestras clínicas que deberían encontrarse estériles, sin necesidad de cultivo previo. Así, se han identificado bacterias patógenas presentes en el líquido cefalorraquídeo 20,21 , líquido sinovial 22 , válvulas cardíacas 23 y sangre [24][25][26] . Aunque la identificación directa a partir de muestras clínicas es una técnica sumamente eficaz, carece de algunas de las ventajas que ofrece el cultivo 11 .…”

Section: Aplicaciones De La Secuenciación Del Arnr 16s En El Diagnóstunclassified

Identificación bacteriana mediante secuenciación del ARNr 16S: fundamento, metodología y aplicaciones en microbiología clínica

Rodicio

Mendoza²

2004

Enferm Infecc Microbiol Clin

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Section: Aplicaciones De La Secuenciación Del Arnr 16s En El Diagnóstunclassified

Identificación bacteriana mediante secuenciación del ARNr 16S: fundamento, metodología y aplicaciones en microbiología clínica

Rodicio

Mendoza²

2004

Enferm Infecc Microbiol Clin

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“…15 One case report has described identification of Fusobacterium species in a brain abscess, and another, Streptococcus pneumoniae in a subdural empyema, by the broad range bacterial PCR approach alone. 16 17 We have previously used broad range bacterial rDNA PCR to analyse 536 clinical samples of various tissues from patients admitted to hospital during the years 1994 to 1997. That work also included samples obtained during neurosurgery, but the patients involved were not further described nor the results specifically discussed.…”

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Aetiological diagnosis of brain abscesses and spinal infections: application of broad range bacterial polymerase chain reaction analysis

Kupila

Rantakokko‐Jalava²,

Jalava³

et al. 2003

Journal of Neurology, Neurosurgery &Amp; Psychiatry

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Objective: To evaluate the usefulness of the broad range bacterial rDNA polymerase chain reaction (PCR) method combined with DNA sequencing in the aetiological diagnosis of intracranial or spinal infections in neurosurgical patients. Methods: In addition to conventional methods, the broad range bacterial PCR approach was applied to examine pus or tissue specimens from cerebral or spinal lesions in patients treated in a neurosurgical unit for a clinical or neuroradiological suspicion of bacterial brain abscess or spondylitis. Results: Among the 44 patients with intracranial or spinal lesions, the final diagnosis suggested bacterial disease in 25 patients, among whom the aetiological agent was identified in 17. A causative bacterial species was identified only by the rDNA PCR method in six cases, by both the PCR methodology and bacterial culture in six cases, and by bacterial culture alone in five. All samples in which a bacterial aetiology was identified only by the PCR approach were taken during antimicrobial treatment, and in three patients the method yielded the diagnosis even after > 12 days of parenteral treatment. One case also identified by the PCR approach alone involved a brain abscess caused by Mycoplasma hominis, which is not readily cultured by routine methods. Conclusions: In patients with brain abscesses and spinal infections, the broad range bacterial rDNA PCR approach may be the only method to provide an aetiological diagnosis when the patient is receiving antimicrobial treatment, or when the causative agent is fastidious.

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“…Es handelt sich dabei einmalwie bei unserem Patienten -um die Detektion von Fusobacterium spp. [7], im zweiten Fall wurde Streptococcus pneumoniae bestimmt [6], während die Kulturen in beiden Fällen kein Bakterienwachstum zeigten. Beide Patienten standen unter einer empirischen Antibiotikatherapie.…”

Section: Diskussionunclassified

“…In einem Fall erbrachte die Sequenzanalyse des PCR-Produkts jedoch kein eindeutiges Resultat. Ein solches Ergebnis ist dann zu erwarten, wenn mehr als ein Erreger vorliegen [7]. Dieses methodische Problem limitiert derzeit die Aussagekraft der Sequenzanalyse im Fall von polymikrobiell verursachten Hirnabszessen.…”

Section: Diskussionunclassified

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Erregerdetektion und -identifikation bei einem Hirnabszess durch molekulargenetische Methoden

et al. 2004

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Brain abscesses are life-threatening and detection and identification of the causative pathogens are crucial for substantiating the diagnosis and for selecting the optimal antibiotic regimen. In approximately 20% of the patients microbiological cultures of abscess material remain sterile. The polymerase chain reaction (PCR) provides a methodological alternative, but data about the use of broad spectrum PCR assays to detect the causative pathogens in brain abscesses are rare. We report on the case of a 65-years-old patient with a brain abscess caused by Fusobacterium spp., which was only diagnosed by broad spectrum PCR. To our knowledge this is the second report about a brain abscess, where Fusobacterium spp. was identified only by broad spectrum PCR and subsequent DNA sequencing.

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Identification of the cause of a brain abscess by direct 16S ribosomal DNA sequencing

Cited by 7 publications

References 9 publications

Identificación bacteriana mediante secuenciación del ARNr 16S: fundamento, metodología y aplicaciones en microbiología clínica

Identificación bacteriana mediante secuenciación del ARNr 16S: fundamento, metodología y aplicaciones en microbiología clínica

Aetiological diagnosis of brain abscesses and spinal infections: application of broad range bacterial polymerase chain reaction analysis

Erregerdetektion und -identifikation bei einem Hirnabszess durch molekulargenetische Methoden

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