Identification of the bioactive components from pH-modified citrus pectin and their inhibitory effects on galectin-3 function

Zhang, Tao; Yu, Liang; Zheng, Yi; Liu, Fengjian; Zhao, Dongyang; Mayo, Kevin H.; Zhou, Yifa; Tai, Guihua

doi:10.1016/j.foodhyd.2016.02.020

Cited by 50 publications

(25 citation statements)

References 44 publications

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“…S4, none of these galactans yielded readable resonances, even though the Gal-3-mediated hemagglutination assay showed them to be inhibitory [22]. One possible explanation is that the interaction between Gal-3 and these galactans is weaker compared to pectins like RG-I-4, p-galactan and MCP, each of which contains multiple galactan side chains [22,26,37]. In the G3H assay, galactans were found to be less potent inhibitors than these pectins [22].…”

Section: Surface Plasmon Resonance (Spr)mentioning

confidence: 95%

“…In this comparative methodology study, we used three pectins (MCP, RG-I-4, and p-galactan) and a series of galactans that represent structural components found in pgalactan, RG-I-4 and MCP [26,27,35,37]. As shown in Fig.…”

Section: Preparation Purity and Size Characterization Of Pectic Commentioning

confidence: 99%

“…and p-galactan, is a mixture of pectic polysaccharides with some of its components binding strongly to Gal-3 and others more weakly [37]. In addition, the G3H assay is a cell-based system, and Gal-3 may interact with multiple glycol-conjugates on the cell surface, in contrast to these other assays which are single molecule-based systems.…”

Section: Gal-3-mediated Hemagglutination (G3h)mentioning

confidence: 99%

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Multiple approaches to assess pectin binding to galectin-3

Zhang

Zheng

Zhao

et al. 2016

International Journal of Biological Macromolecules

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Section: Surface Plasmon Resonance (Spr)mentioning

confidence: 95%

Section: Preparation Purity and Size Characterization Of Pectic Commentioning

confidence: 99%

Section: Gal-3-mediated Hemagglutination (G3h)mentioning

confidence: 99%

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Multiple approaches to assess pectin binding to galectin-3

Zhang

Zheng

Zhao

et al. 2016

International Journal of Biological Macromolecules

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“…MCP's anti-cancer effect is mediated by specific molecular interactions with galectin-3, a β-galactoside-binding lectin with varied biological functions. Extracellular galectin-3 is reported to play a vital role in tumour progression and metastasis [93,94].…”

Section: Citrus Fruitsmentioning

confidence: 99%

Bioactives from Agri-Food Wastes: Present Insights and Future Challenges

2020

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Sustainable utilization of agri-food wastes and by-products for producing value-added products (for cosmetic, pharmaceutical or food industrial applications) provides an opportunity for earning additional income for the dependent industrial sector. Besides, effective valorisation of wastes/by-products can efficiently help in reducing environmental stress by decreasing unwarranted pollution. The major focus of this review is to provide comprehensive information on valorisation of agri-food wastes and by-products with focus laid on bioactive compounds and bioactivity. The review covers the bioactives identified from wastes and by-products of plants (fruits, exotic fruits, vegetables and seeds), animals (dairy and meat) and marine (fish, shellfish seaweeds) resources. Further, insights on the present status and future challenges of sustainably utilizing agri-food wastes/by-products for value addition will be highlighted.

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“…Among NDC from plant-food sources and Gal-3 inhibition, the modified citrus pectin (MCP) is the most studied one [187,[209][210][211]. MCP is a preparation derived from citrus pectin that is modified by high temperature, alteration of pH and/or pectinase treatment, which result in the partial hydrolysis of glycosidic linkages, thereby generating smaller and less ramified NDC structure.…”

Section: Direct Interaction With Galectin-3mentioning

confidence: 99%

Structural-dependent effects of dietary fibers in colon cancer: Focus on dietary fiber naturally changed by the papaya ripening

Prado¹

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PRADO, S. B. R. Structural-dependent effects of dietary fibers in colon cancer: Focus on dietary fiber naturally changed by the papaya ripening. 2019. 327f. Tese (Doutorado) -Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, 2019.Dietary fiber (DF) consumption is related with several healthy benefits such as the decreasing risk of colon cancer development. The DF is not digested by the digestive enzymes and reach to colon where is fermented by the colonic microbiota. The fermentation process releases metabolites as short chain fatty acids (SCFA)such as butyrate, propionate and acetate. Besides the fermentation process, the DF can directly interact with intestinal epithelial cells inducing mechanism that can also be related with the associated DF consumption benefits. The lack of information regarding DF and colon cancer are due to the complexity of both the cancer and the DF structure. The papayas DF are derived from the fruit cell wall, and they are probably naturally modified during ripening through a massive polysaccharide hydrolysis, because papayas show a very fast pulp softening. Due to the lack of information about DF and their beneficial effects to human health as well as the possibility of the natural papaya ripening to modifying the DF presented in the fruit pulp, the present thesis had as the primary objectives: 1) to evaluate how the cell-wall degrading enzymes affect the fruit cell wall solubilization and molecular weight; 2) to investigate the direct effects of the papaya pectin derived from unripe to ripe papayas in cancer cell lines, in galectin-3 interaction and in HEK cells expressing pattern recognition receptors (PRR); 3) to evaluate the human colonic in vitro fermentation using DF from unripe and ripe papayas as substrates; 4) to conduct an in vivo experiment using rats with pre-neoplastic colon lesions while receiving a diet with DF from unripe and ripe papayas. The endopolygalacturonases were the main enzymes acting on the solubilizing papaya cell wall pectin affecting both the papaya firmness and pectin structure. Overall, the papayas DF showed a ripening dependent structure-effects. In the cancer cell lines experiments, the ripe papayas pectin showed a more pronounced effects in inducing cancer cell death, inhibiting cancer cells migration and aggregation, activating PRR as toll-like receptors and inhibiting the prometastatic protein galectin-3. The DF from papayas also showed different aspects in colonic in vitro fermentation regarding the DF utilization by the bacteria and the bacteria abundance profile. Lastly, the animals receiving the diet with the DF from ripe papayas had less aberrant crypt foci in colon than the animals that received the DF from unripe papayas or cellulose (AIN-93G DF). Therefore, the study of papaya DF was carried out both during papaya ripening and its biological effects in vitro and in vivo, generating unprecedented results relating the endogenous biochemical changes of the fruits during maturation with the possible beneficial effects o...

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Identification of the bioactive components from pH-modified citrus pectin and their inhibitory effects on galectin-3 function

Cited by 50 publications

References 44 publications

Multiple approaches to assess pectin binding to galectin-3

Multiple approaches to assess pectin binding to galectin-3

Bioactives from Agri-Food Wastes: Present Insights and Future Challenges

Structural-dependent effects of dietary fibers in colon cancer: Focus on dietary fiber naturally changed by the papaya ripening

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