Identification of the Bacterial Community of Maple Sap by Using Amplified Ribosomal DNA (rDNA) Restriction Analysis and rDNA Sequencing

Lagacé, Luc; Pitre, Mélanie; Jacques, Mario; Roy, Denis

doi:10.1128/aem.70.4.2052-2060.2004

Cited by 106 publications

(69 citation statements)

References 25 publications

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“…Minimum cells of putative clones were used as templates in reaction mixtures during the re-amplification procedure. PCR products of positive clones were subjected to amplified ribosomal DNA restriction analysis (ARDRA) (Lagacé et al 2004) with HaeIII and HhaI (TaKaRa). Clones with identical ARDRA profiles were classified into one operational taxonomic unit (OTU).…”

Section: Standard Curve Of Real-time Pcrmentioning

confidence: 99%

Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China

Zhang

She

Zheng

et al. 2010

Appl Microbiol Biotechnol

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Currently, molecular biologic techniques achieve a great development in studies of soil samples. The objective of this research is to improve methods for microbial prospecting of oil and gas by applying cultureindependent techniques to soil sampled from above a known oil and gas field. Firstly, the community structure of soil bacteria above the Ban 876 Gas and Oil Field was analyzed based on 16S rRNA gene clone libraries. The soil bacteria communities were consistently different along the depth; however, Chloroflexi and Gemmatimonadetes were predominant and methanotrophs were minor in both bacteria libraries (DGS1 and DGS2). Secondly, the numbers of methane-oxidizing bacteria, quantified using a culture-dependent procedure and culture-independent group-specific real-time PCR (RT-PCR), respectively, were inconsistent with a quantify variance of one or two orders of magnitude. Special emphasis was given to the counting advantages of RT-PCR based on the methanotrophic pmoA gene. Finally, the diversity and distribution of methanotrophic communities in the soil samples were analyzed by constructing clone libraries of functional gene. All 508-bp inserts in clones phylogenetically belonged to the methanotrophic pmoA gene with similarities from 83% to 100%. However, most of the similarities were below 96%. Five clone libraries of methanotrophs clearly showed that the anomalous methanotrophs (Methylosinus and Methylocystis) occupy the studied area.

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Section: Standard Curve Of Real-time Pcrmentioning

confidence: 99%

Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China

Zhang

She

Zheng

et al. 2010

Appl Microbiol Biotechnol

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show abstract

“…Then, 200 clones in each clone library were obtained to select positive clones through PCR with the sets of vector-specific primers T7/SP6. PCR products of positive clones were subjected to amplified ribosomal DNA restriction analysis (ARDRA) with HinfI and HaeIII (Fermentas, Lithuania) [20].…”

Section: Construction Of 16s Rrna Gene Librariesmentioning

confidence: 99%

Anaerobic Microbial Diversity and Abundance in Xinjiang low-Temperature Water- Flooding Petroleum Reservoir

Chai¹,

Zhang²,

She³

et al. 2017

JESTR

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The important role of anaerobic microorganisms in oil recovery has attracted attention, but anaerobic microbial diversity and abundance in low-temperature water-flooding oil reservoir has not been studied. To investigate anaerobic microorganisms with diverse physiological and metabolic abilities in a low-temperature water-flooding petroleum reservoir in Xinjiang, China, four types of anaerobic microorganisms were enriched on the selective cultures, and microbial communities in four anaerobic enrichments and production water (PW) were analyzed based on construction of a 16S rRNA gene clone library. Analysis of 16S rRNA gene sequencing and phylogenetic diversity reveals that the reservoir harbours large amounts of anaerobic microorganisms. Arcobacter, Thalassolituus, Azoarcus, and Marinobacterium are predominant groups in the PW clone library. Acidaminobacter, Spirochaete, Spaerochaeta, and Geovibrio are dominant and have the potential to grow using hydrocarbons as carbon sources. Fermentative bacteria mainly belong to Spirochaete; the nitrate-reducing bacteria are Pseudomonas, Acidaminobater, Acetobacterium, Spirochaete, and Spaerochaeta; and the sulfate-reducing bacteria are closely related to Desulfovibrio, Desulfuromonas, Sulfurospirillum, and Spirochaete. Spirochaetes are present in the five samples indicating diverse metabolic abilities and indigenous presence in oil reservoirs. Knowledge of the predominant microbial communities in the reservoir conditions was proposed as a reference in formulating a strategy to enhance oil recovery through injection of selected nutrients or inhibitors to stimulate selective microbes.

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“…Lagace et al (2004) identified the bacterial community of maple trees. A wide variety of the organisms were detected from different groups.…”

Section: Amplified Ribosomal Dna Restriction Analysis (Ardra)mentioning

confidence: 99%

Gel Electrophoresis Based Genetic Fingerprinting Techniques on Environmental Ecology

Çetecioğlu¹,

İnce²,

İnce³

2012

Gel Electrophoresis - Advanced Techniques

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Identification of the Bacterial Community of Maple Sap by Using Amplified Ribosomal DNA (rDNA) Restriction Analysis and rDNA Sequencing

Cited by 106 publications

References 25 publications

Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China

Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China

Anaerobic Microbial Diversity and Abundance in Xinjiang low-Temperature Water- Flooding Petroleum Reservoir

Gel Electrophoresis Based Genetic Fingerprinting Techniques on Environmental Ecology

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