Identification of the ASR gene family from Brachypodium distachyon and functional characterization of BdASR1 in response to drought stress

Wang, Lianzhe; Hu, Wei; Feng, Jialu; Yang, Xiaoyue; Huang, Qi; Xiao, Jiajing; Liu, Yang; Yang, Guangxiao; He, Guoqing

doi:10.1007/s00299-016-1954-6

Cited by 34 publications

(39 citation statements)

References 62 publications

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“…e ASR gene from Ginkgo biloba (GbASR) could be induced by ABA in roots and leaves [25]. e Brachypodium distachyon BdASR genes, except BdASR4, were found upregulated in leaves after ABA treatment [35]. In peach, the * * * * * * * * expression of PpASR could be induced in leaves by ABA [36].…”

Section: Discussionmentioning

confidence: 99%

Abscisic Acid, Stress, and Ripening (TtASR1) Gene as a Functional Marker for Salt Tolerance in Durum Wheat

Hamdi

Brini

Kharrat

et al. 2020

BioMed Research International

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In semiarid Mediterranean agroecosystems, drought and salinity are the main abiotic stresses hampering wheat productivity and yield instability. Abscisic acid, stress, and ripening (ASR) are small plant proteins and play important roles in different biological processes. In the present study, the TtASR1 gene was isolated and characterized for the first time from durum wheat (Tritucum turgidum L. subsp. durum). TtASR1 is a small gene, about 684 bp long, located on chromosome 4AL, encoding a protein of 136 amino acid residues consisting of a histidine-rich N terminus and C-terminal conserved ABA-WDS domain (Pfam PF02496). Our results showed that TtASR1 protein could function as a chaperone-like protein and improve the viability of E. coli under heat and cold stress and increase the Saccharomyces cerevisiae tolerance under salt and osmotic stress. Transcript expression patterns of TtASR1 revealed that ASRs play important roles in abiotic stress responses in diverse organs. Indeed, TtASR1 was upregulated in leaves by different developmental (ABA) and environmental signals (PEG, salt). In cv. Mahmoudi (salt-tolerant Tunisian durum landraces) roots, TtASR1 was upregulated by salt stress, while it was downregulated in cv. Azizi (salt-sensitive Tunisian durum landraces), supporting the implication of this gene in the salt tolerance mechanism. Taken together and after validation in the plant system, the TtASR1 gene may provide a potential functional marker for marker-assisted selection in a durum wheat breeding program for salt tolerance.

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Section: Discussionmentioning

confidence: 99%

Abscisic Acid, Stress, and Ripening (TtASR1) Gene as a Functional Marker for Salt Tolerance in Durum Wheat

Hamdi

Brini

Kharrat

et al. 2020

BioMed Research International

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“…ASRs display different subcellular localizations. Some of these proteins localize to the nucleus (Padmanabhan et al, 1997), while some are detected in both the cytoplasm and nucleus (Kalifa et al, 2004), and some are dispersed throughout the cell (Wang L. et al, 2016), likely reflecting their different functions.…”

Section: Introductionmentioning

confidence: 99%

SiASR4, the Target Gene of SiARDP from Setaria italica, Improves Abiotic Stress Adaption in Plants

Yang

et al. 2017

Front. Plant Sci.

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Drought and other types of abiotic stresses negatively affect plant growth and crop yields. The abscisic acid-, stress-, and ripening-induced (ASR) proteins play important roles in the protection of plants against abiotic stress. However, the regulatory pathway of the gene encoding this protein remains to be elucidated. In this study, the foxtail millet (Setaria italica) ASR gene, SiASR4, was cloned and characterized. SiASR4 localized to the cell nucleus, cytoplasm and cytomembrane, and the protein contained 102 amino acids, including an ABA/WDS (abscisic acid/water-deficit stress) domain, with a molecular mass of 11.5 kDa. The abundance of SiASR4 transcripts increased after treatment with ABA, NaCl, and PEG in foxtail millet seedlings. It has been reported that the S. italica ABA-responsive DRE-binding protein (SiARDP) binds to a DNA sequence with a CCGAC core and that there are five dehydration-responsive element (DRE) motifs within the SiASR4 promoter. Our analyses demonstrated that the SiARDP protein could bind to the SiASR4 promoter in vitro and in vivo. The expression of SiASR4 increased in SiARDP-overexpressing plants. SiASR4-transgenic Arabidopsis and SiASR4-overexpressing foxtail millet exhibited enhanced tolerance to drought and salt stress. Furthermore, the transcription of stress-responsive and reactive oxygen species (ROS) scavenger-associated genes was activated in SiASR4 transgenic plants. Together, these findings show that SiASR4 functions in the adaption to drought and salt stress and is regulated by SiARDP via an ABA-dependent pathway.

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“…ASRs from other plants species have been reported to possess transactivation activity [4,9]. To assess the transactivation activity of IpASR, its complete coding region was fused in-frame with the GAL4 DNA binding domain of the pGBKT7 vector in this study.…”

Section: Resultsmentioning

confidence: 99%

“…The first member of ASR , tomato Asr1 , was identified by screening a tomato fruit cDNA library using a cDNA differential hybridization approach, with accumulated transcripts observed in the ripening fruit, water-stressed leaves, or under ABA treatment, hence its name [6]. Following this, a fairly large number of ASR orthologs were characterized from many plant species along with the advance of the Plant Genome Project [7,8,9,10]. …”

Section: Introductionmentioning

confidence: 99%

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Ipomoea pes-caprae IpASR Improves Salinity and Drought Tolerance in Transgenic Escherichia coli and Arabidopsis

Zheng

Zhang

et al. 2018

IJMS

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Ipomoea pes-caprae L. is an extremophile halophyte with strong adaptability to seawater and drought. It is widely used in the ecological restoration of coastal areas or degraded islands in tropical and subtropical regions. In this study, a new abscisic acid, stress and ripening (ASR) gene, IpASR, was reported, and is mainly associated with biological functions involved in salt and drought tolerance. Sequence analysis of IpASR showed that this protein contains an ABA/WDS (abscisic acid/water deficit stress) domain, which is a common feature of all plant ASR members. Overexpression of IpASR improved Escherichia coli growth performance compared with the control under abiotic stress treatment. The transgenic overexpressing IpASR Arabidopsis showed higher tolerance to salt and drought stress than the wild type and lower accumulation of hydrogen peroxide (H2O2) and superoxide (O2−) accompanied by increased antioxidant enzyme activity in vivo. IpASR exhibits transcription factor’s activity. Therefore, the overexpression of IpASR in Arabidopsis is supposed to influence the expression of some genes involved in anti-oxidative and abiotic stresses. The results indicate that IpASR is involved in the plant response to salt and drought and probably acts as a reactive oxygen species scavenger or transcription factor, and therefore influences physiological processes associated with various abiotic stresses in plants.

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Identification of the ASR gene family from Brachypodium distachyon and functional characterization of BdASR1 in response to drought stress

Cited by 34 publications

References 62 publications

Abscisic Acid, Stress, and Ripening (TtASR1) Gene as a Functional Marker for Salt Tolerance in Durum Wheat

Abscisic Acid, Stress, and Ripening (TtASR1) Gene as a Functional Marker for Salt Tolerance in Durum Wheat

SiASR4, the Target Gene of SiARDP from Setaria italica, Improves Abiotic Stress Adaption in Plants

Ipomoea pes-caprae IpASR Improves Salinity and Drought Tolerance in Transgenic Escherichia coli and Arabidopsis

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