Identification of the AQP8-miR-92a network associated with the aggressive traits of colorectal cancer

Zhang, Hui; Du, Wen Bo; Guo, Xiao; Wang, Li Kang; Cheng, Jun; Wei, Li Jing

doi:10.1016/j.bbrc.2020.04.055

Cited by 11 publications

(6 citation statements)

References 15 publications

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“…The proliferation and invasion of colon cancer cells can be inhibited by the overexpression of AQP8 a member of the aquaporin family 101 . Consequently, Zhang et al 102 reported that the expression level of AQP8 was substantially reduced in CRC tissues comparable to normal tissues, which is consistent with our results and having high levels of AQP8 was related to increased survivability in patients suffering from CRC. One of the key transporters that excretes oxalate is SLC26A6, which is mostly expressed in the small intestine comparison SLC26A3 can regulate oxalate absorption in ileum, cecum and colon 103 .…”

Section: Discussionsupporting

confidence: 92%

Identification of GUCA2A and COL3A1 as prognostic biomarkers in colorectal cancer by integrating analysis of RNA-Seq data and qRT-PCR validation

Hosseini,

Nemati

2023

Sci Rep

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By 2030, it is anticipated that there will be 2.2 million new instances of colorectal cancer worldwide, along with 1.1 million yearly deaths. Therefore, it is critical to develop novel biomarkers that could help in CRC early detection. We performed an integrated analysis of four RNA-Seq data sets and TCGA datasets in this study to find novel biomarkers for diagnostic, prediction, and as potential therapeutic for this malignancy, as well as to determine the molecular mechanisms of CRC carcinogenesis. Four RNA-Seq datasets of colorectal cancer were downloaded from the Sequence Read Archive (SRA) database. The metaSeq package was used to integrate differentially expressed genes (DEGs). The protein–protein interaction (PPI) network of the DEGs was constructed using the string platform, and hub genes were identified using the cytoscape software. The gene ontology and KEGG pathway enrichment analysis were performed using enrichR package. Gene diagnostic sensitivity and its association to clinicopathological characteristics were demonstrated by statistical approaches. By using qRT-PCR, GUCA2A and COL3A1 were examined in colon cancer and rectal cancer. We identified 5037 differentially expressed genes, including (4752 upregulated, 285 downregulated) across the studies between CRC and normal tissues. Gene ontology and KEGG pathway analyses showed that the highest proportion of up-regulated DEGs was involved in RNA binding and RNA transport. Integral component of plasma membrane and mineral absorption pathways were identified as containing down-regulated DEGs. Similar expression patterns for GUCA2A and COL3A1 were seen in qRT-PCR and integrated RNA-Seq analysis. Additionally, this study demonstrated that GUCA2A and COL3A1 may play a significant role in the development of CRC.

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Section: Discussionsupporting

confidence: 92%

Identification of GUCA2A and COL3A1 as prognostic biomarkers in colorectal cancer by integrating analysis of RNA-Seq data and qRT-PCR validation

Hosseini,

Nemati

2023

Sci Rep

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“…miR-92a promotes the growth and invasion of CRC cells by negatively regulating expression of AQP8. 31 In our analysis, AQP8 was found to have an effect on overall survival in patients with colorectal cancer. AQP8 plays an extremely important role in the occurrence and development of constipation and colorectal cancer.…”

Section: Discussionmentioning

confidence: 57%

Analysis of Key Genes for Slow Transit Constipation Based on RNA Sequencing

Yu¹,

Yang²,

Guan³

et al. 2022

IJGM

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This study aims to identify key genes in slow transit constipation (STC). We also sought to explore the potential link between STC and colorectal cancer. Patients and Methods: mRNA expression profiles were obtained by RNA sequencing, and differentially expressed genes were identified. Functional enrichment analysis and a protein-protein interaction (PPI) network was explored, and differentially expressed genes common to STC and colorectal cancer were examined. Analysis of the effect of constipation and colorectal cancer common genes on the overall survival of colorectal cancer patients based on GEPIA database. Results: Functional enrichment showed that significantly different genes are related to lymphocyte chemotaxis, positive regulation of inflammatory response, cellular response to tumor necrosis factor, extracellular region, extracellular space and chemokine activity. The hub gene for STC was found in the PPI network. In addition, AQP8 and CFD were common differential genes for STC and colorectal cancer. AQP8 affects overall survival in patients with colorectal cancer. Conclusion: Our findings will contribute to understanding the pathology of STC at the molecular level, with the first discovery that AQP8 may be a hub gene in the transition from STC to colorectal cancer.

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“…However, it was reported that AQP8 expression is lower in colon cancer tissues than in normal colon tissues [ 32 ]. AQP8 overexpression in the colorectal cancer cell lines SW480 and HT-29 inhibited the growth and invasion of colorectal cancer cells by inactivating the PI3K/AKT signaling pathway and inhibiting PCDH7 expression [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

AQP8 promotes glioma proliferation and growth, possibly through the ROS/PTEN/AKT signaling pathway

Hao,

Huajun,

Zhen

et al. 2023

BMC Cancer

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Background The aquaporin (AQP) family of proteins has been implicated in the proliferation and growth of gliomas. Expression of AQP8 is higher in human glioma tissues than in normal brain tissues and is positively correlated with the pathological grade of glioma, suggesting that this protein is also involved in the proliferation and growth of glioma. However, the mechanism by which AQP8 promotes the proliferation and growth of glioma remains unclear. This study aimed to investigate the mechanism and role of abnormal AQP8 expression in glioma development. Methods The dCas9-SAM and CRISPR/Cas9 techniques were used to construct viruses with overexpressed and knocked down AQP8, respectively, and infect A172 and U251 cell lines. The effects of AQP8 on the proliferation and growth of glioma and its mechanism via the intracellular reactive oxygen species (ROS) level were observed using cell clone, transwell, flow cytometry, Hoechst, western blotting, immunofluorescence, and real-time quantitative polymerase chain reaction assays. A nude mouse tumor model was also established. Results Overexpression of AQP8 resulted in an increased number of cell clones and cell proliferation, enhanced cell invasion and migration, decreased apoptosis and phosphatase and tensin homolog (PTEN) expression, and increased phosphorylated serine/threonine protein kinase (p-AKT) expression and ROS level, whereas the AQP8 knockdown groups showed opposite results. In the animal experiments, the AQP8 overexpression group had higher tumor volume and weight, whereas the AQP8 knockdown group had lower tumor volume and weight compared with those parameters measured in the control group. Conclusions Our results preliminary suggest that AQP8 overexpression alters the ROS/PTEN/AKT signaling pathway, promoting the proliferation, migration, and invasion of gliomas. Therefore, AQP8 may be a potential therapeutic target in gliomas.

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Identification of the AQP8-miR-92a network associated with the aggressive traits of colorectal cancer

Cited by 11 publications

References 15 publications

Identification of GUCA2A and COL3A1 as prognostic biomarkers in colorectal cancer by integrating analysis of RNA-Seq data and qRT-PCR validation

Identification of GUCA2A and COL3A1 as prognostic biomarkers in colorectal cancer by integrating analysis of RNA-Seq data and qRT-PCR validation

Analysis of Key Genes for Slow Transit Constipation Based on RNA Sequencing

AQP8 promotes glioma proliferation and growth, possibly through the ROS/PTEN/AKT signaling pathway

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