Identification of the antigenic determinants of factors 8, 9, and 34 of genus Candida

Abstract

We investigated the antigenic determinants of factorsadherence of Candida cells to mammalian tissues during the 8, 9, and 34 of the genus Candida among pathogenic yeasts by initial step of infection [11]. Therefore, the cell wall compoenzyme-linked immunosorbent assay (ELISA) using mannans of nents containing mannose residues in the o~ configuration

NMR assignment of the galactomannan of Candida lipolytica

“…L showed a strong reactivity to factors 1 and 9 sera. These results support the finding that factor 9 serum contains an antibody against the a-l,6-1inked backbone mannose units which have no side chains [10,11,27,28]. These results indicate that the use of antigenic factors for the classification of the structure of the cell wall polysaccharides is not perfect, because some of the factor sera have been known to contain antibodies against several antigenic structures [22,29].…”

Structural and immunochemical characterization of β-1,2-linked mannobiosyl phosphate residue in the cell wall mannan of Candida glabrata

“…9 have been reported to not be monospecific. Although the main recognition site is the branched oligomannosyl side-chain that possesses nonreducing terminal α-1,6-and β-1,2-linked mannose residues , another site is namely a linear α-1,6-linked backbone (Ataoglu et al 1993;Kobayashi et al 1996).…”

“…The specific recognition sites of sera nos. 1, 4, 8, 13 b, and 34 correspond to various α-linked side-chains (Funayama et al 1983;Kobayashi et al 1996). On the other hand, the oligomannosyl side-chains containing a β-1,2 linkage act as antigenic determinants of sera nos.…”

Amended structure of side chains in a cell wall mannan from Candida albicans serotype A strain grown in yeast extract-Sabouraud liquid medium under acidic conditions: detection of the branched side chains corresponding to antigenic factor 4

“…The cross‐peak of non‐reducing terminal α‐1,3‐linked mannose residues in the branched side chain appeared at a similar position to these residues in the linear side chain (cross‐peak 5, H‐1, 5.135 ppm; H‐2, 4.070 ppm). However, the fact that the total peak dimensions of two H‐1 signals at 5.376 and at 5.135 ppm were in agreement with that of the H‐1 signal at 4.924 ppm corresponding to the non‐reducing terminal α‐1,6‐linked mannose residues, indicating that A2 mannan does not contain linear side chains possessing the α‐1,3‐linked mannose residues corresponding to antigenic factors 13b[12] and 34[13], Manα1–2Manα1–3Manα1‐(2Manα1‐) 2 2Man and Manα1–3Manα1‐(2Manα1‐) 2 2Man. Table 2 shows the molar ratios of side chains corresponding to the antigenic factors in A6 and A2 mannans, which were calculated based on the dimension of H‐1 signals in the 1 H‐NMR spectra.…”