Identification of the absorbed ingredients and metabolites in rats after an intravenous administration of Tanreqing injection using high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

Abstract

The metabolic profiles of Tanreqing injection, which is a traditional Chinese medicine recommended for complementary administration to treat a novel coronavirus, have remained unclear, which inhibit the understanding of the effective chemical compounds of Tanreqing injection. In this study, a sensitive highperformance liquid chromatography quadrupole time-of-flight mass spectrometry method was used to identify the compounds and metabolites in various biosamples, including plasma, bile, liver, lung, kidney, urine, and feces, following the intravenous administration of Tanreqing injection in rats. A total of 89 compounds were characterized in the biosamples of Tanreqing injection-treated rats including 25 precursor constituents and 64 metabolites. Nine flavonoid compounds, twelve phenolic acids, and four iridoid glycosides were identified in the rats. Their metabolites were mainly produced by

Pharmacokinetics of baicalin and oroxyloside in plasma and different tissues of rats after transnasal aerosol inhalation and intravenous injection of Tanreqing

“…The botanical drugs were extracted by water extraction (HQ, JYH, and LQ) and were purified by alcohol sedimentation, hyperfiltration (HQ), saponification (XD), and acid hydrolysis (SYJ), respectively ( Mu, 2011 ). The chemical composition of TRQ includes flavonoids, iridoids, phenolic acids, amino acids, phenethyl alcohol glycosides, lignans, and steroids ( Li C et al, 2019 ; Xie et al, 2021 ). TRQ possesses anti-inflammatory, antibacterial, antiviral, antitumor, and other pharmacological effects.…”

Characterization of the chemical constituents and in vivo metabolic profile of Scutellaria barbata D. Don by ultra high performance liquid chromatography with high‐resolution mass spectrometry

“…As shown in Supporting information Table S2 and S3, the numbers of the identified prototype compounds and metabolites in rat plasma, urine, and feces were different. These may be partially contributed to the fact that the concentrations and the MS fragments abundances of these identified compounds may be different in rat plasma, urine, and fecal sample [26]. Also the preferred metabolism organs (liver, gastrointestinal tract, gut microbiota) of these compounds existing in S. barbata may be different [27].…”

Introductory Chapter: A Tool for Aided Advanced Diagnostics and Deep View into Biological Sample

“…A number of analytical methods for mass spectrometry in clinical laboratories were developed during the recent past, and the development gains additional momentum as the CoVID-19 pandemics holds the mankind in grip and methods for fast HPLC-MS have been developed and applied [6][7][8][9][10][11][12][13][14][15].…”